Ultra-low-temperature Pleurotus eryngii spawn preservation method for factory production

A technology of ultra-low temperature preservation and Pleurotus eryngii, which is applied in botany equipment and methods, applications, gardening, etc., and can solve the problems of unsuitable strains for long-term preservation, deterioration, and poor preservation effect

Inactive Publication Date: 2014-04-16
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] There are many preservation methods for existing strains, commonly used are low-temperature preservation, liquid paraffin preservation, wheat grain preservation, salt water preservation, etc. For mushroom strains (2n) with rapid changes in physiological characteristics, using the above preservation method

Method used

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Examples

Experimental program
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Embodiment 2

[0028] This embodiment includes the following steps:

[0029] (1) Preservation of strain preparation

[0030] Routinely inoculate the strains on ordinary culture medium, grow typical colonies after 3 days of culture at 25±1°C, and cut out the inoculated bacteria blocks on concentric circles;

[0031] (2) Protective agent preparation

[0032] Use dimethyl sulfoxide as a protective agent, dilute dimethyl sulfoxide with distilled water to a dimethyl sulfoxide aqueous solution with a volume concentration of 12% before use, put it into a triangular flask, plug the bottle with a cotton plug, and sterilize under high pressure Use after cooling;

[0033] (3) Save operation steps

[0034] 1) Use an inoculation needle for aseptic operation to cut the agar with hyphae from the mother seed into soybean-sized slices, put them into sterile test tubes, and put 5 agar slices in each sterile test tube, and then add the agar in step (2). The above protective agent is used until the agar sli...

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Abstract

An ultra-low-temperature Pleurotus eryngii spawn preservation method for factory production includes 1, preserved spawn preparation; 2, protective agent preparation; and 3, preserving operation. The step of preserving operation includes (1), slicing agar of mother spawn source with mycelia into the bean sizes through an inoculating needle by sterile operation, placing the 4 to 6 agar slices into one sterile test tube, then adding the protective agent till covering the agar slices, sealing the sterile test tube, and marking through mark pens; (2), punching holes at the bottom, cover and side wall of plastic bottles, placing the sterile test tubes to the plastic bottles, then placing the plastic bottles the a ultra-low-temperature freezer, and decreasing the temperature to -80 to -150 DEG C at the speed of decreasing 1+-0.1 DEG C in every minute. By the aid of the ultra-low-temperature Pleurotus eryngii spawn preservation method for factory production, the Pleurotus eryngii spawn can be maintained in extreme slow metabolism state for 3 to 5 years generally, and excellent genetic characteristics can be maintained constantly.

Description

technical field [0001] The invention relates to a method for preserving pleurotus eryngii strains, in particular to a method for ultra-low temperature preservation of pleurotus eryngii strains for industrial production. Background technique [0002] Pleurotus eryngii strain is an important factor in the industrial production of Pleurotus eryngii. The internal quality of the strain and the pros and cons of genetic traits determine the production capacity of edible fungi, which are internal factors. Under the same cultivation conditions, improved varieties can increase production by more than 30% compared with ordinary varieties, or even doubled. However, if the selected good strains are not well managed, they will cause decline, or be polluted by miscellaneous bacteria, resulting in reduced production. Therefore, the preservation of strains and the selection of strains are of equal importance. In production, due to the large number of original species and cultivars, it is d...

Claims

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Application Information

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IPC IPC(8): A01G1/04
Inventor 夏志兰阳国秀易恢满
Owner HUNAN AGRICULTURAL UNIV
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