Culture medium and tissue culture method for tissue culture of Craterostigma pumilum Hochst
A tissue culture and rooting medium technology, applied in horticultural methods, botanical equipment and methods, applications, etc., can solve the problems of low multiplication coefficient and long reproduction cycle, and achieve high plant survival rate, fast reproduction speed, maintenance Effects of genetic traits
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Embodiment 1
[0043] The formula of the medium for the tissue culture of the dwarf stylindrical grass of the present embodiment is as follows:
[0044] Seedling breeding medium: 6g / L agar+30g / L sucrose+MS medium. Cluster bud induction medium: 6g / L agar + 30g / L sucrose + 0.1mg / L kinetin + 0.1mg / L naphthaleneacetic acid + MS medium. Proliferation medium: 6g / L agar+30g / L sucrose+0.1mg / L 6-benzylaminoadenine+0.05mg / L naphthaleneacetic acid+MS medium. The rooting medium is 1 / 2MS medium.
Embodiment 2
[0046] The formula of the medium for the tissue culture of the dwarf stylindrical grass of the present embodiment is as follows:
[0047] Seedling breeding medium: 6g / L agar+30g / L sucrose+MS medium. Cluster bud induction medium: 6g / L agar + 30g / L sucrose + 0.1mg / L kinetin + 0mg / L naphthaleneacetic acid + MS medium. Proliferation medium: 6g / L agar+30g / L sucrose+0.1mg / L 6-benzylaminoadenine+0.1mg / L naphthaleneacetic acid+MS medium. The rooting medium is 1 / 2MS medium.
Embodiment 3
[0049] The formula of the medium for the tissue culture of the dwarf stylindrical grass of the present embodiment is as follows:
[0050] Seedling breeding medium: 6g / L agar+30g / L sucrose+MS medium. Cluster bud induction medium: 6g / L agar + 30g / L sucrose + 0.5mg / L kinetin + 0.05mg / L naphthaleneacetic acid + MS medium. Proliferation medium: 6g / L agar+30g / L sucrose+0.5mg / L 6-benzylaminoadenine+0.2mg / L naphthaleneacetic acid+MS medium. The rooting medium is 1 / 2MS medium.
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