Culture medium and tissue culture method for tissue culture of Craterostigma pumilum Hochst

A tissue culture and rooting medium technology, applied in horticultural methods, botanical equipment and methods, applications, etc., can solve the problems of low multiplication coefficient and long reproduction cycle, and achieve high plant survival rate, fast reproduction speed, maintenance Effects of genetic traits

Active Publication Date: 2021-08-24
SHENZHEN XIANHU BOTANICAL GARDEN ADMINISTRATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Aiming at the problem that the propagation mode of dwarf stylindrix in the prior art has a long propagation cycle, low multiplication coefficient, and is difficult to meet the current popularization and application requirements, the present invention provides a medium for tissue culture of dwarf stylindrical, tissue culture method

Method used

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  • Culture medium and tissue culture method for tissue culture of Craterostigma pumilum Hochst
  • Culture medium and tissue culture method for tissue culture of Craterostigma pumilum Hochst
  • Culture medium and tissue culture method for tissue culture of Craterostigma pumilum Hochst

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Embodiment 1

[0043] The formula of the medium for the tissue culture of the dwarf stylindrical grass of the present embodiment is as follows:

[0044] Seedling breeding medium: 6g / L agar+30g / L sucrose+MS medium. Cluster bud induction medium: 6g / L agar + 30g / L sucrose + 0.1mg / L kinetin + 0.1mg / L naphthaleneacetic acid + MS medium. Proliferation medium: 6g / L agar+30g / L sucrose+0.1mg / L 6-benzylaminoadenine+0.05mg / L naphthaleneacetic acid+MS medium. The rooting medium is 1 / 2MS medium.

Embodiment 2

[0046] The formula of the medium for the tissue culture of the dwarf stylindrical grass of the present embodiment is as follows:

[0047] Seedling breeding medium: 6g / L agar+30g / L sucrose+MS medium. Cluster bud induction medium: 6g / L agar + 30g / L sucrose + 0.1mg / L kinetin + 0mg / L naphthaleneacetic acid + MS medium. Proliferation medium: 6g / L agar+30g / L sucrose+0.1mg / L 6-benzylaminoadenine+0.1mg / L naphthaleneacetic acid+MS medium. The rooting medium is 1 / 2MS medium.

Embodiment 3

[0049] The formula of the medium for the tissue culture of the dwarf stylindrical grass of the present embodiment is as follows:

[0050] Seedling breeding medium: 6g / L agar+30g / L sucrose+MS medium. Cluster bud induction medium: 6g / L agar + 30g / L sucrose + 0.5mg / L kinetin + 0.05mg / L naphthaleneacetic acid + MS medium. Proliferation medium: 6g / L agar+30g / L sucrose+0.5mg / L 6-benzylaminoadenine+0.2mg / L naphthaleneacetic acid+MS medium. The rooting medium is 1 / 2MS medium.

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Abstract

The invention relates to the technical field of agricultural production, and provides a culture medium and a tissue culture method for tissue culture of Craterostigma pumilum Hochst. The culture medium for tissue culture of the Craterostigma pumilum Hochst comprises a seedling breeding culture medium, a cluster bud induction culture medium, a proliferation culture medium and a rooting culture medium, wherein all the media are based on cheap and easily available MS culture media and are prepared by adding appropriate exogenous hormones or raw materials. The formula of each medium is scientific and reasonable, the media can be correspondingly used for each stage of tissue culture of the Craterostigma pumilum Hochst, the culture process is high in propagation speed, short in period and high in plant survival rate which can reach about 90%, the excellent hereditary character of the Craterostigma pumilum Hochst variety can be well kept, and market popularization and garden application of the variety are favorably promoted.

Description

technical field [0001] The invention relates to the technical field of agricultural production, in particular to a culture medium and a tissue culture method for dwarf stylindrical tissue culture. Background technique [0002] In recent years, African ornamental plants have been widely used in modern landscaping. Dwarf stylus is a small perennial herb of the motherwort family and syllium, mainly distributed in southern Africa, southern Arabian Peninsula and India. The delicate and lovely dwarf stylindrical has high ornamental value, and is suitable for many purposes such as potting, garden flower bed landscaping, rock slope protection, forest undercover and plant micro-landscape production, and has a good garden application prospect. [0003] Under natural conditions, the dwarf stylinia introduced from Africa to South China can bear fruit in large quantities, but the seedlings grow slowly after sowing and emergence; the root system is vulnerable to damage when propagated by...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 程纹万涛廖一颖王美萍蒙林平刘志明
Owner SHENZHEN XIANHU BOTANICAL GARDEN ADMINISTRATION
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