Hybrid method for improving cold resistance of Ilex rotunda

Abstract

The invention discloses a hybrid method for improving the cold resistance of Ilex rotunda. The method includes the following steps of (1) pollen storage: taking a good Ilex cornuta varfortunei plant as a female parent from April to May, using a sterilized conical flask to collect male pollen, and filling the conical flask with nitrogen for storage after low temperature baking; (2) hybridization: activating the stored pollen firstly from March to April in the second year, when pollination needs to be conducted, using a sodium hypochlorite solution and a plant extract solution to spray Ilex flowers respectively, and then conducting pollination; (3) seed treatment: placing first-generation seeds into an ammoniation pool to conduct ammoniation treatment, and then conducting temperature changing treatment; (4) repeated hybridization: according to the way of step 2, conducting hybridization cultivation on the processed first-generation seeds and Ilex cornuta varfortunei to obtain second-generation seeds, conducting hybridization cultivation on the second-generation seeds and the Ilex cornuta varfortunei to obtain third-generation seeds, and then conducting intraspecific breeding cultivation on the third-generation seeds to obtain hybridized Ilex rotunda seeds; (5) cultivation: conducting greenhouse cultivation on the hybridized Ilex rotunda seeds after the treatment of step 3.

Description

technical field [0001] The invention belongs to the technical field of iron holly planting, and in particular relates to a hybridization method for improving the cold resistance of iron holly. Background technique [0002] The thornless wolfberry is an evergreen shrub of the holly genus. The tree species is luxuriant and has a strange leaf shape. The fruit turns from green to red after ripening, and the branches are covered with red fruits. Later, it can have high ornamental value and is often used as a Christmas tree; thornless Chinese wolfberry is fond of light, moist and fertile soil, it has high cold resistance, and can grow normally at a temperature of -8--10°C, and has good adaptability. strong. Iron holly is an evergreen shrub of the Ilex family, also known as Jiubing and Xiongdan wood. Its fruit is red when ripe. Wild tree species are mostly born in evergreen broad-leaved forests and forest edge areas on hillsides at an altitude of 400-1100 meters. Currently, it is ...

AI Technical Summary

Problems solved by technology

[0003] The present invention aims at the existing problems: iron holly is shade-tolerant, likes to be born in warm and humid climate and loose and fertile soil, is suitable for growing in subtropical warm and humid regions, has relatively poor cold resistance, and is not suitable for planting and breeding in relatively cold regio...