The invention discloses PTC graphene heating printing ink and a preparation method thereof. The PTC graphene heating printing ink is prepared from the following components in percentages by mass: 50% to 70% of a binder, 9% to 21% of a solvent, 1% to 3% of an additive, 10% to 20% of a conductive filler and 5% to 20% of a PTC functional material. The preparation method comprises the following steps: (1) firstly stirring and dispersing the binder, the solvent and the additive for 20 to 30 min, then adding the PTC functional material into stirring equipment for stirring for 8 to 12 min, and finally adding the conductive filler for uniform stirring to obtain a coarse paste; and (2) carrying out four to six times of three-roller grinding on the coarse paste so as to obtain the PTC graphene heating printing ink. The invention also discloses a heating film. The heating film comprises an organic polymer base material film, the PTC graphene heating printing ink, a conductive silver paste, conductive copper foil and an organic polymer adhesive film. The PTC function of the graphene heating film is increased, the potential safety hazard problem of an existing graphene heating film is eliminated, and the practicability is improved.