Method for establishing human amniotic fluid stem cell line
A stem cell line and establishment method technology, applied in the field of cell line establishment, can solve the problems of reduced number, limited cell proliferation ability, low purity, etc., and achieve the effect of stable performance and good genetic traits
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[0037] The invention provides a method for establishing a human amniotic fluid stem cell line, the method comprising the following steps:
[0038]1. Amniotic fluid stem cell separation steps: collect amniotic fluid in the second and third trimester from the obstetrics and gynecology department of the hospital, record in detail the pregnancy time, amniotic fluid appearance, amniotic fluid volume, and the size of cell clumps after centrifugation, and store them at 4°C and bring them back to the laboratory. Filter with a 200-mesh filter to remove larger tissue fragments, then centrifuge at 1000r / min for 6min, discard the supernatant, and collect the cells. Then add PBS(-) solution containing 1% double antibody, mix well, treat at 37°C for 10min, then centrifuge, collect cells, repeat treatment with 1% double antibody PBS(-) solution 3 times.
[0039] 2. Amniotic fluid stem cell culture steps: 1) Add the amniotic fluid cells isolated in the previous step to the primary amniotic fl...
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