Rapid separation device and method for cysts of plant cyst nematodes

Abstract

The invention discloses a rapid separation device for cysts of plant cyst nematodes. The device is composed of an outer cylinder and an inner cylinder, wherein the inner cylinder is fixed into the outer cylinder, and the outer cylinder and the inner cylinder are each in a cylindrical shape with an opening in the upper end and a sealed bottom; and one or more filtering windows are formed in the upper portion of the inner cylinder, the inner cylinder communicates with the outer cylinder through the filtering windows, the filtering windows are covered with 18-25-mesh screens, and a circle of adsorption filter paper which is tightly attached to and surrounds the inner wall of the outer cylinder is arranged on the inner wall of the outer cylinder. The invention further discloses a method for rapidly separating the cysts of the plant cyst nematodes by using the device. The device is low in cost and high in separation speed, one sample is separated within 10min, the collection efficiency is high, and the obtained sample to be observed is cleaner.

Description

technical field

[0001] The invention belongs to the technical field of separation and identification of plant nematodes, and in particular relates to a device for quickly separating cysts of nematodes of the plant cyst genus or Coccidioides genus and a method for rapidly separating cysts by using the device. Background technique

[0002] Plant nematodes belong to the nematode phylum of invertebrates and can parasitize plant roots, stems, leaves, fruits, seeds and other parts. There are many types and serious harm. According to incomplete statistics, the economic losses of agricultural and forestry crops caused by plant nematodes in the world are more than hundreds of billions of yuan every year. In the catalog of foreign plant quarantine pests published by my country in 2007, 20 species (genera) of nematodes are quarantine nematodes, and the actual number of species is as many as 200 or more.

[0003] Among the plant parasitic nematodes, cyst nematodes (including cyst nemat...

Examples

Embodiment 1

[0042] like figure 1 As shown, the quick separation device for plant cyst nematode cysts consists of an outer cylinder 1 and an inner cylinder 2, the inner cylinder 2 is fixed inside the outer cylinder 1, and both the outer cylinder 1 and the inner cylinder 2 are round with an open upper end and a sealed bottom. Cylindrical; the upper part of the inner cylinder 2 is provided with more than one filter window 3, the filter window 3 communicates with the inner cylinder 2 and the outer cylinder 1, the filter window 3 is covered with a 18-25 mesh screen 4, the outer The inner wall of the barrel is provided with a circle of adsorption filter paper 5 which is closely attached to and surrounds the inner wall.

[0043] Further, the screen 4 can be fixed on the filter window 3 or detachably connected with the filter window 3 . The size of the screen cloth 4 should be greater than the size of the filter window 3, so that the screen cloth 4 will completely cover the filter window 3.

[...

Embodiment 2

[0057] Utilize the device of embodiment 2 to carry out the rapid separation of plant cyst nematodes, the steps are:

[0058] 1. Weigh the soil sample to be tested and put it into the inner cylinder; put the filter paper roll into the outer cylinder, and wet the filter paper with water first, so that the filter paper cylinder is wrapped around the inner wall of the outer cylinder to form an adsorption filter paper;

[0059] 2. Add water to the inner cylinder to 2 / 3~3 / 4 of the height below the bottom of the filter window of the inner cylinder, and gently stir the mixture in the inner cylinder with a glass rod for 5-10 times;

[0060] 3. Continue to slowly add water to the inner cylinder, so that the water in the inner cylinder slightly exceeds the bottom of the filter window, so that the floating objects on the surface overflow from the screen of the filter window to the outer cylinder;

[0061] 4. Continue to gently stir the inner cylinder with a glass rod, and slowly add water...

technical field

[0001] The invention belongs to the technical field of separation and identification of plant nematodes, and in particular relates to a device for quickly separating cysts of nematodes of the plant cyst genus or Coccidioides genus and a method for rapidly separating cysts by using the device. Background technique

[0002] Plant nematodes belong to the nematode phylum of invertebrates and can parasitize plant roots, stems, leaves, fruits, seeds and other parts. There are many types and serious harm. According to incomplete statistics, the economic losses of agricultural and forestry crops caused by plant nematodes in the world are more than hundreds of billions of yuan every year. In the catalog of foreign plant quarantine pests published by my country in 2007, 20 species (genera) of nematodes are quarantine nematodes, and the actual number of species is as many as 200 or more.

[0003] Among the plant parasitic nematodes, cyst nematodes (including cyst nemat...

Examples

Embodiment 1

[0042] like figure 1 As shown, the quick separation device for plant cyst nematode cysts consists of an outer cylinder 1 and an inner cylinder 2, the inner cylinder 2 is fixed inside the outer cylinder 1, and both the outer cylinder 1 and the inner cylinder 2 are round with an open upper end and a sealed bottom. Cylindrical; the upper part of the inner cylinder 2 is provided with more than one filter window 3, the filter window 3 communicates with the inner cylinder 2 and the outer cylinder 1, the filter window 3 is covered with a 18-25 mesh screen 4, the outer The inner wall of the barrel is provided with a circle of adsorption filter paper 5 which is closely attached to and surrounds the inner wall.

[0043] Further, the screen 4 can be fixed on the filter window 3 or detachably connected with the filter window 3 . The size of the screen cloth 4 should be greater than the size of the filter window 3, so that the screen cloth 4 will completely cover the filter window 3.

[...

Embodiment 2

[0057] Utilize the device of embodiment 2 to carry out the rapid separation of plant cyst nematodes, the steps are:

[0058] 1. Weigh the soil sample to be tested and put it into the inner cylinder; put the filter paper roll into the outer cylinder, and wet the filter paper with water first, so that the filter paper cylinder is wrapped around the inner wall of the outer cylinder to form an adsorption filter paper;

[0059] 2. Add water to the inner cylinder to 2 / 3~3 / 4 of the height below the bottom of the filter window of the inner cylinder, and gently stir the mixture in the inner cylinder with a glass rod for 5-10 times;

[0060] 3. Continue to slowly add water to the inner cylinder, so that the water in the inner cylinder slightly exceeds the bottom of the filter window, so that the floating objects on the surface overflow from the screen of the filter window to the outer cylinder;

[0061] 4. Continue to gently stir the inner cylinder with a glass rod, and slowly add water...

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