54 results about "Plant quarantine" patented technology

The invention provides a fusarium specific gene segment and application of the fusarium specific gene segment in rapid detection of fusarium species. In particular, the present invention provides a fusarium specific segment. Furthermore, the invention also provides a primer pair for detecting fusarium species, and the primer pair is used for amplifying the fusarium specific segment disclosed by the invention. Furthermore, the invention also provides an application of the fusarium specific segment or the primer pair provided by the invention in detecting fusarium species. Through the fusarium specific section, different species in fusarium can be accurately distinguished, and the fusarium specific section can be applied to the field of plant quarantine to realize rapid and accurate detection of fusarium species.

The utility model discloses a plant quarantine tissue slicing device convenient to clean, which comprises a base frame, a driving motor is fixedly arranged on the base frame, a synchronous slicing device is arranged on the base frame, and the synchronous slicing device comprises a first guide rail, a first sliding block, a threaded rod, a platform sliding block, a workbench and a driving mechanism, the first guide rail is fixedly installed on the base frame, the first sliding block is connected to the first guide rail in a sliding mode, the threaded rod is rotationally connected to the base frame, the platform sliding block is connected to the threaded rod in a threaded mode, and the lower side of the platform sliding block is fixedly installed on the upper side of the first sliding block. And one end of the threaded rod is fixedly connected to an output shaft of the driving motor. According to the slicing machine, slicing and feeding operations can be synchronously performed, so that the thickness of each slice is uniform, automatic cleaning work can be performed while the working table is reset, and the slicing efficiency is improved.

The invention discloses a CRISPR (clustered regularly interspaced short palindromic repeats) / Cas-based kit for isothermal rapid visual detection of tomato mosaic virus and a detection method of the CRISPR / Cas-based kit. The invention belongs to the technical field of plant quarantine, and particularly relates to a CRISPR / Cas-based kit for isothermal rapid visual detection of tomato mosaic virus and a detection method thereof. The composition for identifying the tomato mosaic virus comprises crRNA specific to nucleic acid to be detected, an RT-RPA primer pair, Cas12 protein and a fluorescent reporter probe, the RT-RPA primer pair is composed of a primer ToMV-F and a primer ToMV-R, and the fluorescent reporter probe is a single-chain RNA with one end connected with a fluorophore and the other end connected with a quenching group. According to the present invention, the rapid, accurate and visual detection of the to-be-detected sample can be achieved, the advantages of good specificity, high sensitivity, convenient operation, high detection efficiency and the like are provided, and the basic requirements of field and port detection can be met.

The invention belongs to the field of agriculture and plant quarantine, and particularly relates to a method for detecting phomopsis helianthi based on RPA / CRISPR-Cas12a, which comprises the following steps: S1, designing a detection composition: designing a composition for detecting phomopsis helianthi, the composition comprising a specific primer RPA-F / R nucleic acid sequence, a specific crRNA nucleic acid sequence and probes FL-ssDNA and LF-ssDNA; s2, extracting DNA: extracting the genome DNA of the diseased tissue of the sunflower to be detected; s3, DNA isothermal amplification: taking the genome DNA obtained in the step S1 as a template, adding a specific primer RPA-F1 / R1 into the template, and carrying out RPA amplification to obtain an amplification product; s4, CRISPR / Cas12a reaction: the amplification product obtained in the S2 and the crRNA are respectively mixed with FL-ssDNA and LF-ssDNA probes for a cleavage reaction, and a reaction product FL and a reaction product LF are obtained; and S5, visual detection: respectively placing the reaction product FL and the reaction product LF in a fluorescent PCR instrument and on a Cas12 special detection test strip for detection. The method is based on an RPA / CRISPR-Cas12a technology, by designing a specific primer and a crRNA nucleic acid sequence, the detection duration of the phomopsis helianthi is greatly shortened, the detection sensitivity is improved, and the method has very high use value in detection scenes such as customs and the like.

Herbicide phytosanitary compositions formulated in the form of microemulsions, with low content of surfactants, high compatibility in ultra-low volume spray solutions, and methods for formulating said compositions and tank mixes.

The invention discloses a pine wood nematode disease rapid detection method, and relates to the field of forestry plant quarantine and pest and disease damage detection, a portable integrated detection device is used for executing the following steps: S1, sample collection: a miniature drilling unit of the device automatically drills into a to-be-detected pine tree trunk to take a wood chip sample; s2, sample pretreatment: conveying and weighing a wood chip sample, transferring the wood chip sample to a crushing and extracting chamber after the wood chip sample reaches a target weight, adding a lysis solution, heating, crushing and extracting to obtain an extracting solution; s3, sample transfer: injecting the extraction liquid into a micro-fluidic chip mechanism by using a diaphragm pump; s4, nucleic acid purification and isothermal amplification: carrying out nucleic acid purification on the extract in a micro-fluidic chip mechanism, and carrying out a nucleic acid isothermal amplification reaction at a constant temperature; and S5, optical detection and result analysis: carrying out real-time fluorescence monitoring on a nucleic acid isothermal amplification process, obtaining and processing a fluorescence signal, calculating the estimated concentration of the pine wood nematodes based on an algorithm model, and evaluating an infection risk level in combination with forest stand background information. The method realizes on-site rapid and accurate detection, and can meet field rapid detection requirements.

The invention relates to a plant protection composition comprising at least: a paraffinic hydrocarbon fraction comprising isoparaffins, normal paraffins, and naphthenes; an alkoxylated oleic acid; a sorbitan ester; and an alkoxylated isotridecanol. The invention also relates to the uses of this composition for treating diseases, particularly those caused by fungi or insects, or for fertilizing plants, and to methods of applying plant protection products to crops, comprising at least one application step, preferably by sprinkling, spraying, or dredging using a drone, of the plant protection composition according to the invention.

The invention discloses an LAMP (loop-mediated isothermal amplification) primer group, a kit and a method for detecting four quarantine viruses and application of the LAMP primer group. Belongs to the technical field of molecular biology and plant quarantine. Aiming at conserved gene regions of bean pod mottle virus, tobacco ringspot virus, tomato ringspot virus and southern bean mosaic virus, a specific target sequence is screened through bioinformatics analysis, an LAMP primer group is designed, and the nucleotide sequence of the LAMP primer group is shown as SEQ ID NO.1-22. Furthermore, the screened primer group is combined with a micro-fluidic chip technology to construct a detection kit capable of synchronously detecting the four viruses, so that the specific identification of the four target viruses is realized. The kit has the advantages of rapidness, sensitivity, specificity, simplicity and convenience in operation and the like, the sensitivity can reach 10 <-10 > Copies / mu L, and the kit is suitable for high-throughput rapid screening of quarantine viruses in imported soybeans on port sites and has important significance in guaranteeing national biosafety.

The utility model relates to the technical field of plant quarantine, in particular to a nematode suction device. Comprising a vacuum exhaust pipe, a connecting pipe and a glass suction pipe which are sequentially connected from top to bottom, the upper portion of the connecting pipe is sleeved with a glass sleeve, the glass sleeve is sleeved with a rubber sleeve, the upper end of the rubber sleeve is tightly sleeved with the lower end of the vacuum exhaust pipe, so that the vacuum exhaust pipe and the connecting pipe are fixedly connected into a whole, and the lower end of the connecting pipe is sleeved with a grid. The lower end of the grid is connected with the nematode adhesive film, and the glass suction pipe is connected to the lower portion of the connecting pipe in a sleeved mode and located between the rubber sleeve and the connecting pipe. The nematode suction device is reasonable in structure, ingenious in design, simple, convenient and easy to operate, an inexperienced person can suck nematodes easily, rapidly and efficiently, and the success rate of follow-up experiments and the overall working efficiency are greatly improved.

The utility model discloses a plant quarantine sample extraction device, which relates to the technical field of plant quarantine and comprises a bottom plate, and a rotating disc is rotatably arranged on the upper end surface of the bottom plate; limiting grooves are annularly and evenly formed in the upper end face of the rotating disc. Test tubes are placed in the limiting grooves; supporting frames are fixedly installed on the front and rear sides of the upper end face of the bottom plate. A mixing tank is fixedly mounted at the top ends of the two supporting frames; the upper end face of the mixing tank is communicated with a rectangular feeding pipe and a liquid inlet pipe, and a mixing assembly is arranged on the mixing tank; the lower end surface of the mixing tank is fixedly connected with a discharging pipe; a valve is mounted on the discharging pipe, and a rotating disc, a shifting plate, a mixing tank, the discharging pipe and the valve are arranged; the rotating disc is rotated to drive the plurality of test tubes to rotate, so that the solution in the mixing tank sequentially flows into the plurality of test tubes; the operation steps are simplified, and the working efficiency is improved. The driving assembly drives the two stirring pieces to rotate in opposite directions in the mixing tank; further, the mixing efficiency of the plant needing sample extraction and the solution is improved.

The utility model relates to the technical field of sample extraction equipment, and discloses sample extraction equipment for plant quarantine, which comprises a workbench, a grinding table is arranged on the upper surface of the workbench, a sampling table is fixedly connected to the upper surface of the workbench, a dismounting assembly is arranged at the top of the workbench, and a pressing assembly is arranged at the top of the workbench. The pressing assembly is used for pressing a plant sample, the dismounting assembly comprises lantern rings, the lantern rings are fixedly connected to the top of the workbench, a plurality of insertion columns are fixedly connected to the side wall of the grinding table, the insertion columns are slidably connected to the interiors of the lantern rings, first sliding blocks are slidably connected to the interiors of the insertion columns, and springs are arranged in the insertion columns. And one end of the spring is fixedly connected to the side wall of the grinding table. According to the grinding table disclosed by the utility model, the grinding table can be quickly disassembled through a convenient disassembly mode between the insertion column and the lantern ring, so that the grinding table can be comprehensively and carefully cleaned, and no sample residue and cross contamination are ensured.

This invention belongs to the field of plant quarantine technology and discloses an IoT-driven real-time monitoring and early warning device for plant quarantine pests. The on-site monitoring terminal collects multi-dimensional on-site data related to pests; the edge computing module performs real-time preprocessing, feature extraction, and preliminary anomaly detection on the on-site data; the IoT transmission module enables bidirectional data transmission between the edge computing module and the cloud-based early warning platform; and the cloud-based early warning platform performs in-depth analysis of the received data, pest identification and density assessment, and executes tiered early warning and data management. This invention provides comprehensive monitoring dimensions, enabling monitoring of all types of pests at all times: integrating multi-sensor detection units, image acquisition units, and trapping units, and combining pheromone, vibration, spore capture, and image acquisition methods, it achieves monitoring of various plant quarantine pests such as insects, fungi, and bacteria.

The invention relates to a mixture of essential oils comprising, relative to the total volume of the essential oil mixture, 2 to 35% by volume of citronellal; and 2 to 35% by volume of menthol. The invention also relates to the use of this mixture of essential oils in plant protection compositions for the pesticide treatment of plants, particularly for the control of aphids. No figure.

The present application provides a Fusarium-specific gene segment and its use in rapid detection of Fusarium species. Specifically, the present application provides a Fusarium-specific segment. Further, the present application also provides a primer pair for detecting Fusarium species, which amplifies the Fusarium-specific segment of the present application. Still further, the present application also provides the use of the Fusarium-specific segment or primer pair of the present application in detecting Fusarium species. Through the Fusarium-specific segment of the present application, different species within the Fusarium genus can be accurately distinguished, which can be applied in the field of plant quarantine to achieve rapid and accurate detection of Fusarium species.

The utility model relates to the field of forestry plant quarantine samples, and discloses a forestry plant quarantine sample collecting device which comprises a handle, a fixed shell is arranged on one side of the handle, a movable groove is formed in one side of the fixed shell, a sliding block is connected in the movable groove in a sliding mode, and a detachable sampling cutter cylinder is fixedly connected to one side of the sliding block. The lower end of the sampling cutter cylinder is an edged end, the lower end of the fixed shell is fixedly connected with a supporting seat, and the upper end of the supporting seat is fixedly connected with a supporting boss matched with the sampling cutter cylinder; an air pipe is slidably inserted into one end, far away from the edged edge, of the sampling cutter cylinder, an air bag is fixedly connected to the upper end of the air pipe, a one-way air valve is arranged on the air bag, an adsorption block slidably connected into the sampling cutter cylinder is fixedly connected to the lower end of the air pipe, an air cavity is formed in the adsorption block, and an adsorption hole is formed in the bottom surface of the adsorption block. Compared with the prior art, the plant leaf slicing device has the advantages that plant leaves can be quickly sliced, and time and labor are saved.

The invention specifically relates to the technical field of computers and the technical field of biological information, and discloses a method and a system for constructing a multi-dimensional database for image recognition of foreign and local diseases and insect pests, and the method comprises the steps: obtaining basic information, hazard feature information and other information of foreign and local diseases and insect pests through self-sampling and public resource collection and processing; analyzing the collected original data set and the scanning image set, and carrying out image preprocessing, data set division, model evaluation and improved construction on the three-dimensional internal and external models and the image recognition model; a foreign and local pest and disease damage multi-dimensional information access rule is constructed, a foreign and local pest and disease damage image recognition multi-dimensional database is developed, data support can be provided for research and development of technologies such as rapid and accurate detection and one-time detection, and the system can become a port entry plant quarantine information support system. The method can certainly play an important role in improving port quarantine and extraneous potential pest control ability, effectively reducing extraneous quarantine pest introduction and protecting agricultural production and ecological safety.

The invention relates to the technical field of moisture meters, and specifically to a grain moisture meter for plant quarantine, comprising a body and a measuring cup arranged on the body, and also comprising a sleeve ring, wherein the sleeve ring is arranged on the outside of the measuring cup; an outer tube, wherein the outer tube is arranged on the measuring cup and used in conjunction with the sleeve ring; an inner tube, wherein the inner tube is arranged inside the outer tube and used in conjunction with the outer tube; and a plurality of function boards. The grain moisture meter for plant quarantine enables a worker to control the rotation of the function board by using a material dropping unit when measuring the moisture content of the grain, thereby achieving blocking of the bottom of the inner tube and dropping all the grains in the inner tube into the measuring cup, avoiding the dumping of the grains, simplifying the steps, and facilitating reducing the workload of the worker. A rotary squeezing unit is used to drive the inner tube and the function board to move, so as to flatten the grains dropped into the measuring cup and press the grains, thereby making the measurement more accurate.

The invention discloses a DNA (Deoxyribonucleic Acid) molecule, an RT-PCR (Reverse Transcription-Polymerase Chain Reaction) primer group and application of the RT-PCR primer group in tomato ringspot virus detection. Belongs to the technical field of plant quarantine. A gene segment SEQ ID NO.1 with a high conservative characteristic is selected from RNA1 of ToRSV, the gene segment is used as a target gene, a corresponding amplification primer is designed to detect the tomato ringspot virus, an optimal reaction condition is researched through a series of condition exploration and optimization, the detection sensitivity and specificity are greatly improved, and the detection result is more accurate. The detection period is shortened, the detection process is accelerated, technical support is provided for shortening the detection period of an inspection and quarantine department, and a foundation is laid for accurately and rapidly identifying pathogens.

The present application belongs to the technical field of plant quarantine, and particularly relates to a plant quarantine pest intelligent trapping device integrated with image recognition functions, which comprises a trapping main body module, an image acquisition and recognition module, an intelligent control module, a data transmission module and a power supply module, and each module is electrically connected with the intelligent control module through a line. The present application realizes integrated intelligent operation: the functions of trapping, image acquisition, real-time recognition, statistics, early warning and data uploading are integrated, the whole-process monitoring of quarantine pests can be completed without manual intervention, the cost of manual inspection and recognition is greatly reduced, and the work efficiency of quarantine is improved.

The invention provides an RPA (recombinase polymerase amplification) primer group, a kit and a method for detecting the alternaria brassicae of broccoli, which are used for detecting the alternaria brassicae of broccoli. The primer group comprises specific primers F1 / R1, F1 / R1 and F2 / R1, and the sizes of theoretical amplification products of the primer group are 204bp, 187bp and 189bp. Specific target genes are screened out through comparative genomics, RPA primers are designed, and a constant-temperature (39 DEG C) and rapid (20 minutes) detection method is established. The primer provided by the invention has strong specificity, is only effective to the brassica oleracea var. Brassicae, and has no cross reaction with sibling species; the sensitivity is high, the detection lower limit is 0.08 ng / mu L, and the sensitivity is 100 times higher than that of conventional PCR. The actual sample verifies that the detection accuracy reaches 100%. The method is easy and convenient to operate, rapid in detection and suitable for field early diagnosis, plant quarantine and disease monitoring of the pathogenic bacteria.

The present invention discloses a multiplex RT-PCR primer set, kit and detection method for simultaneously detecting WSMV, MDMV, MCMV and WHPV, belonging to the technical field of plant quarantine. The primer set, kit and detection method of the present invention can simultaneously detect four viruses, namely WSMV, MDMV, MCMV and WHPV at one time. Through a series of condition exploration and optimization, an optimal reaction condition is developed, which greatly improves the accuracy and sensitivity of detection, shortens the detection cycle from the original 4 days to 1-2 days, speeds up the detection process, provides technical support for shortening the detection cycle of inspection and quarantine departments, and lays a foundation for accurately and rapidly identifying pathogens in the wild.

The application discloses a target gene for detecting pea foot rot, a primer set and a detection kit, and belongs to the technical field of plant quarantine. The application innovatively develops a rapid detection technology system based on specific molecular markers in view of the core pain points of long detection period (traditional method >= 20 days) and difficult identification of close species (such as D. pinodes and A. pisi) in the quarantine identification of Didymella pinodella. By screening a single-copy gene sequence unique to D. pinodella and combining PCR technology, a whole-process system of "direct sample detection-target enrichment-signal amplification" is established, and the pathogen detection can be completed within 8 hours, the efficiency is improved by 96% compared with the traditional method, the efficiency is greatly improved, and the close species can be effectively distinguished, the customs clearance efficiency is greatly improved, and technical support is provided for the detection of pea foot rot and the breeding of pea disease resistance.

The utility model discloses an animal and plant quarantine organism transfer device which comprises a transfer box, handles are installed at the two ends of the transfer box, lifting rods are rotatably installed at the two ends of the transfer box, a supporting mechanism is arranged in the transfer box, and the supporting mechanism can support and transfer animals and plants for quarantine. A supporting mechanism is arranged in a transfer box for animal and plant quarantine, small animals are stably supported through a transparent box and a supporting net plate, a bottom box is arranged below the supporting net plate, generated excrement and urine penetrate through meshes of the supporting net plate to fall into the bottom box, and organisms and excrement are separated; excrement and urine generated by organisms are intensively placed, so that pollution is reduced; and meanwhile, the first partition plate and the second partition plate are arranged in the transparent box to separate the internal space of the transparent box, so that the small animals can be independently isolated while being transferred, and the infection risk is reduced.

The application discloses a method for identifying Ectropis oblique and Ectropis grisescens, and belongs to the technical field of molecular biology. The application takes Ectropis oblique and Ectropis grisescens as research objects, systematically screens diagnostic different sites in the whole genome based on population genome resequencing data, and develops a nucleic gene molecular marker which can be used for rapid and accurate identification of close species. COI Compared with the mitochondrial markers in the prior art, the method has wider applicability and higher genetic variation sensitivity at the species and population levels. The molecular identification method established in the application provides reliable technical support for accurate identification of close pests and application of the close pests in plant quarantine and monitoring.

The invention provides a universal primer for rapidly detecting fusarium species and application of the universal primer. Specifically, the invention provides a primer for detecting fusarium species, and the primer is used for amplifying a fusarium specific section. Furthermore, the invention also provides an application of the universal primer of the fusarium species in detecting the fusarium species. By means of the universal primer of fusarium, different species in fusarium can be accurately distinguished, the universal primer can be applied to the field of plant quarantine, and rapid and accurate detection of fusarium species is achieved.

The invention discloses a pine wood nematode wood sample batch separation device, and relates to the technical field of plant quarantine, the pine wood nematode wood sample batch separation device comprises a first base and a partition plate; the second base is fixedly installed on the rear side face of the first base, the height of the first base is 0.25 m, the height of the second base is 0.6 m, the first base and the second base are combined in a stepped mode, the partition plates are evenly fixed in the first base and the second base, round holes are evenly formed in the top face of the first base and the top face of the second base, and the partition plates are arranged in the round holes. According to the device for separating the wood samples of the pine wood nematodes in batches, the plurality of glass funnels can be placed in the plurality of round holes, so that the samples of different batches can be treated at the same time, the operation is simple, the operation is convenient, and the efficiency is high. By rotating the screw rod, the pine wood nematode wood sample can be tightly pressed in the glass funnel by the pressing plate, so that the pine wood nematode wood sample is prevented from floating upwards to influence the experiment effect.

This invention relates to the field of tissue sectioning and sampling technology, and discloses a tissue sectioning device for plant quarantine. The device includes a hand-cranked drive assembly, a piston flow control assembly, an adjustment assembly, a feedback deceleration assembly, and a transfer assembly. The lifting block is linked to the piston block through the piston rod, and during the cutting stroke, it pulsarily presses liquid into the liquid flow chamber next to the slicing blade, generating a pulsed liquid flow synchronized with the slicing rhythm, realizing non-contact guiding and transferring of the wax strip. When cutting hard plant tissue, causing a sudden increase in cutting load, the load increases the pressure in the piston cylinder and pushes the adjustment assembly to switch the flow path. The feedback deceleration assembly converts the pressurized liquid into a normal clamping force of the push block on the turntable, thereby increasing the torque required for the rocker wheel to continue rotating and reducing the risk of impact damage during the cutting of hard tissue. This invention organically combines pulsed liquid flow transfer with hydraulic resistance increase and deceleration, providing a slicing solution with strong sample protection for plant quarantine.

The invention discloses a target gene, a primer group and a detection kit for detecting pea foot rot, and belongs to the technical field of plant quarantine. The invention creatively develops a rapid detection technology system based on a specific molecular marker aiming at the core pain points of long detection period (the detection period is longer than or equal to 20 days in a traditional method), difficulty in identification of sibling species (such as D.pinodes and A.pisi) and the like in quarantine identification of Didymellinosporium petiolatum (Didymellinosporium petiolatum (Didymellinosporium petiolatum (Didymellinosporium petiolatum (Didymellinosporium petiolatum (Didymellinosporium)). By screening a unique single copy gene sequence of D.pinodella and combining a PCR technology, a sample direct detection-target enrichment-signal amplification whole process system is established, pathogen detection can be completed within 8 hours, compared with a traditional method, the efficiency is improved by 96%, the efficiency is greatly improved, near-source species can be effectively distinguished, the customs clearance efficiency is greatly improved, and the method is suitable for large-scale popularization and application. The technical support is provided for the detection of the pea foot rot and the pea disease-resistant breeding.

The utility model relates to the technical field of plant quarantine, and discloses a sample collection device for plant quarantine, which comprises a quarantine sample collection box, a partition plate is arranged in the quarantine sample collection box, and the partition plate in the quarantine sample collection box divides the quarantine sample collection box into a sample storage area and a quarantine tool storage area. A semi-I-shaped sliding block on one side of a disinfection spraying head slides on I-shaped sliding steel to stably support movement of the disinfection spraying head, one end of a limiting rod slides in a square sliding groove to stably support movement of a forward and reverse rotation motor, the disinfection spraying head can be driven to move in a reciprocating mode, and under the action of a lengthened water hose, the disinfection spraying head can be driven to move forwards and backwards. Disinfectant fluid in the disinfectant fluid tank is sprayed to disinfect the quarantine tools at the top end of the placement rack, the quarantine tools are dried under the action of the drying equipment, and the quarantine tools are sterilized under the action of the ultraviolet sterilization lamp, so that quarantine samples can be conveniently collected and used next time.

The application relates to the technical field of plant quarantine, in particular to an imported grain weed rapid quarantine method based on DNA barcodes of compositae plants. The method aims to solve the problems of high missing detection rate and insufficient identification capacity when identifying highly degraded DNA samples. The scheme comprises the following steps: performing low-depth shotgun sequencing on total DNA of grain samples and filtering background sequences; extracting sequence and evolution features from short fragments by using a pre-trained double-channel deep feature extraction network; decoupling the fragment features into conservation, position coding and degradation features by using a decoupling-collaborative reconstruction network, and reconstructing a synthetic feature sequence representing a complete barcode; and finally comparing the sequence with a standard feature database, calculating an identification confidence, and outputting an identification conclusion. The application is especially suitable for rapid and accurate identification of compositae weed seeds with severely degraded DNA in imported grains.