38 results about "Plant quarantine" patented technology

The invention provides a fusarium specific gene segment and application of the fusarium specific gene segment in rapid detection of fusarium species. In particular, the present invention provides a fusarium specific segment. Furthermore, the invention also provides a primer pair for detecting fusarium species, and the primer pair is used for amplifying the fusarium specific segment disclosed by the invention. Furthermore, the invention also provides an application of the fusarium specific segment or the primer pair provided by the invention in detecting fusarium species. Through the fusarium specific section, different species in fusarium can be accurately distinguished, and the fusarium specific section can be applied to the field of plant quarantine to realize rapid and accurate detection of fusarium species.

The invention belongs to the field of agriculture and plant quarantine, and particularly relates to a method for detecting phomopsis helianthi based on RPA / CRISPR-Cas12a, which comprises the following steps: S1, designing a detection composition: designing a composition for detecting phomopsis helianthi, the composition comprising a specific primer RPA-F / R nucleic acid sequence, a specific crRNA nucleic acid sequence and probes FL-ssDNA and LF-ssDNA; s2, extracting DNA: extracting the genome DNA of the diseased tissue of the sunflower to be detected; s3, DNA isothermal amplification: taking the genome DNA obtained in the step S1 as a template, adding a specific primer RPA-F1 / R1 into the template, and carrying out RPA amplification to obtain an amplification product; s4, CRISPR / Cas12a reaction: the amplification product obtained in the S2 and the crRNA are respectively mixed with FL-ssDNA and LF-ssDNA probes for a cleavage reaction, and a reaction product FL and a reaction product LF are obtained; and S5, visual detection: respectively placing the reaction product FL and the reaction product LF in a fluorescent PCR instrument and on a Cas12 special detection test strip for detection. The method is based on an RPA / CRISPR-Cas12a technology, by designing a specific primer and a crRNA nucleic acid sequence, the detection duration of the phomopsis helianthi is greatly shortened, the detection sensitivity is improved, and the method has very high use value in detection scenes such as customs and the like.

Herbicide phytosanitary compositions formulated in the form of microemulsions, with low content of surfactants, high compatibility in ultra-low volume spray solutions, and methods for formulating said compositions and tank mixes.

The invention discloses a pine wood nematode disease rapid detection method, and relates to the field of forestry plant quarantine and pest and disease damage detection, a portable integrated detection device is used for executing the following steps: S1, sample collection: a miniature drilling unit of the device automatically drills into a to-be-detected pine tree trunk to take a wood chip sample; s2, sample pretreatment: conveying and weighing a wood chip sample, transferring the wood chip sample to a crushing and extracting chamber after the wood chip sample reaches a target weight, adding a lysis solution, heating, crushing and extracting to obtain an extracting solution; s3, sample transfer: injecting the extraction liquid into a micro-fluidic chip mechanism by using a diaphragm pump; s4, nucleic acid purification and isothermal amplification: carrying out nucleic acid purification on the extract in a micro-fluidic chip mechanism, and carrying out a nucleic acid isothermal amplification reaction at a constant temperature; and S5, optical detection and result analysis: carrying out real-time fluorescence monitoring on a nucleic acid isothermal amplification process, obtaining and processing a fluorescence signal, calculating the estimated concentration of the pine wood nematodes based on an algorithm model, and evaluating an infection risk level in combination with forest stand background information. The method realizes on-site rapid and accurate detection, and can meet field rapid detection requirements.

The invention discloses an LAMP (loop-mediated isothermal amplification) primer group, a kit and a method for detecting four quarantine viruses and application of the LAMP primer group. Belongs to the technical field of molecular biology and plant quarantine. Aiming at conserved gene regions of bean pod mottle virus, tobacco ringspot virus, tomato ringspot virus and southern bean mosaic virus, a specific target sequence is screened through bioinformatics analysis, an LAMP primer group is designed, and the nucleotide sequence of the LAMP primer group is shown as SEQ ID NO.1-22. Furthermore, the screened primer group is combined with a micro-fluidic chip technology to construct a detection kit capable of synchronously detecting the four viruses, so that the specific identification of the four target viruses is realized. The kit has the advantages of rapidness, sensitivity, specificity, simplicity and convenience in operation and the like, the sensitivity can reach 10 <-10 > Copies / mu L, and the kit is suitable for high-throughput rapid screening of quarantine viruses in imported soybeans on port sites and has important significance in guaranteeing national biosafety.

The utility model relates to the technical field of plant quarantine, in particular to a nematode suction device. Comprising a vacuum exhaust pipe, a connecting pipe and a glass suction pipe which are sequentially connected from top to bottom, the upper portion of the connecting pipe is sleeved with a glass sleeve, the glass sleeve is sleeved with a rubber sleeve, the upper end of the rubber sleeve is tightly sleeved with the lower end of the vacuum exhaust pipe, so that the vacuum exhaust pipe and the connecting pipe are fixedly connected into a whole, and the lower end of the connecting pipe is sleeved with a grid. The lower end of the grid is connected with the nematode adhesive film, and the glass suction pipe is connected to the lower portion of the connecting pipe in a sleeved mode and located between the rubber sleeve and the connecting pipe. The nematode suction device is reasonable in structure, ingenious in design, simple, convenient and easy to operate, an inexperienced person can suck nematodes easily, rapidly and efficiently, and the success rate of follow-up experiments and the overall working efficiency are greatly improved.

The utility model discloses a plant quarantine sample extraction device, which relates to the technical field of plant quarantine and comprises a bottom plate, and a rotating disc is rotatably arranged on the upper end surface of the bottom plate; limiting grooves are annularly and evenly formed in the upper end face of the rotating disc. Test tubes are placed in the limiting grooves; supporting frames are fixedly installed on the front and rear sides of the upper end face of the bottom plate. A mixing tank is fixedly mounted at the top ends of the two supporting frames; the upper end face of the mixing tank is communicated with a rectangular feeding pipe and a liquid inlet pipe, and a mixing assembly is arranged on the mixing tank; the lower end surface of the mixing tank is fixedly connected with a discharging pipe; a valve is mounted on the discharging pipe, and a rotating disc, a shifting plate, a mixing tank, the discharging pipe and the valve are arranged; the rotating disc is rotated to drive the plurality of test tubes to rotate, so that the solution in the mixing tank sequentially flows into the plurality of test tubes; the operation steps are simplified, and the working efficiency is improved. The driving assembly drives the two stirring pieces to rotate in opposite directions in the mixing tank; further, the mixing efficiency of the plant needing sample extraction and the solution is improved.

This invention belongs to the field of plant quarantine technology and discloses an IoT-driven real-time monitoring and early warning device for plant quarantine pests. The on-site monitoring terminal collects multi-dimensional on-site data related to pests; the edge computing module performs real-time preprocessing, feature extraction, and preliminary anomaly detection on the on-site data; the IoT transmission module enables bidirectional data transmission between the edge computing module and the cloud-based early warning platform; and the cloud-based early warning platform performs in-depth analysis of the received data, pest identification and density assessment, and executes tiered early warning and data management. This invention provides comprehensive monitoring dimensions, enabling monitoring of all types of pests at all times: integrating multi-sensor detection units, image acquisition units, and trapping units, and combining pheromone, vibration, spore capture, and image acquisition methods, it achieves monitoring of various plant quarantine pests such as insects, fungi, and bacteria.

The present application provides a Fusarium-specific gene segment and its use in rapid detection of Fusarium species. Specifically, the present application provides a Fusarium-specific segment. Further, the present application also provides a primer pair for detecting Fusarium species, which amplifies the Fusarium-specific segment of the present application. Still further, the present application also provides the use of the Fusarium-specific segment or primer pair of the present application in detecting Fusarium species. Through the Fusarium-specific segment of the present application, different species within the Fusarium genus can be accurately distinguished, which can be applied in the field of plant quarantine to achieve rapid and accurate detection of Fusarium species.

The utility model relates to the field of forestry plant quarantine samples, and discloses a forestry plant quarantine sample collecting device which comprises a handle, a fixed shell is arranged on one side of the handle, a movable groove is formed in one side of the fixed shell, a sliding block is connected in the movable groove in a sliding mode, and a detachable sampling cutter cylinder is fixedly connected to one side of the sliding block. The lower end of the sampling cutter cylinder is an edged end, the lower end of the fixed shell is fixedly connected with a supporting seat, and the upper end of the supporting seat is fixedly connected with a supporting boss matched with the sampling cutter cylinder; an air pipe is slidably inserted into one end, far away from the edged edge, of the sampling cutter cylinder, an air bag is fixedly connected to the upper end of the air pipe, a one-way air valve is arranged on the air bag, an adsorption block slidably connected into the sampling cutter cylinder is fixedly connected to the lower end of the air pipe, an air cavity is formed in the adsorption block, and an adsorption hole is formed in the bottom surface of the adsorption block. Compared with the prior art, the plant leaf slicing device has the advantages that plant leaves can be quickly sliced, and time and labor are saved.

The invention discloses a DNA (Deoxyribonucleic Acid) molecule, an RT-PCR (Reverse Transcription-Polymerase Chain Reaction) primer group and application of the RT-PCR primer group in tomato ringspot virus detection. Belongs to the technical field of plant quarantine. A gene segment SEQ ID NO.1 with a high conservative characteristic is selected from RNA1 of ToRSV, the gene segment is used as a target gene, a corresponding amplification primer is designed to detect the tomato ringspot virus, an optimal reaction condition is researched through a series of condition exploration and optimization, the detection sensitivity and specificity are greatly improved, and the detection result is more accurate. The detection period is shortened, the detection process is accelerated, technical support is provided for shortening the detection period of an inspection and quarantine department, and a foundation is laid for accurately and rapidly identifying pathogens.

The present application belongs to the technical field of plant quarantine, and particularly relates to a plant quarantine pest intelligent trapping device integrated with image recognition functions, which comprises a trapping main body module, an image acquisition and recognition module, an intelligent control module, a data transmission module and a power supply module, and each module is electrically connected with the intelligent control module through a line. The present application realizes integrated intelligent operation: the functions of trapping, image acquisition, real-time recognition, statistics, early warning and data uploading are integrated, the whole-process monitoring of quarantine pests can be completed without manual intervention, the cost of manual inspection and recognition is greatly reduced, and the work efficiency of quarantine is improved.

The invention provides an RPA (recombinase polymerase amplification) primer group, a kit and a method for detecting the alternaria brassicae of broccoli, which are used for detecting the alternaria brassicae of broccoli. The primer group comprises specific primers F1 / R1, F1 / R1 and F2 / R1, and the sizes of theoretical amplification products of the primer group are 204bp, 187bp and 189bp. Specific target genes are screened out through comparative genomics, RPA primers are designed, and a constant-temperature (39 DEG C) and rapid (20 minutes) detection method is established. The primer provided by the invention has strong specificity, is only effective to the brassica oleracea var. Brassicae, and has no cross reaction with sibling species; the sensitivity is high, the detection lower limit is 0.08 ng / mu L, and the sensitivity is 100 times higher than that of conventional PCR. The actual sample verifies that the detection accuracy reaches 100%. The method is easy and convenient to operate, rapid in detection and suitable for field early diagnosis, plant quarantine and disease monitoring of the pathogenic bacteria.

The present invention discloses a multiplex RT-PCR primer set, kit and detection method for simultaneously detecting WSMV, MDMV, MCMV and WHPV, belonging to the technical field of plant quarantine. The primer set, kit and detection method of the present invention can simultaneously detect four viruses, namely WSMV, MDMV, MCMV and WHPV at one time. Through a series of condition exploration and optimization, an optimal reaction condition is developed, which greatly improves the accuracy and sensitivity of detection, shortens the detection cycle from the original 4 days to 1-2 days, speeds up the detection process, provides technical support for shortening the detection cycle of inspection and quarantine departments, and lays a foundation for accurately and rapidly identifying pathogens in the wild.

The application discloses a target gene for detecting pea foot rot, a primer set and a detection kit, and belongs to the technical field of plant quarantine. The application innovatively develops a rapid detection technology system based on specific molecular markers in view of the core pain points of long detection period (traditional method >= 20 days) and difficult identification of close species (such as D. pinodes and A. pisi) in the quarantine identification of Didymella pinodella. By screening a single-copy gene sequence unique to D. pinodella and combining PCR technology, a whole-process system of "direct sample detection-target enrichment-signal amplification" is established, and the pathogen detection can be completed within 8 hours, the efficiency is improved by 96% compared with the traditional method, the efficiency is greatly improved, and the close species can be effectively distinguished, the customs clearance efficiency is greatly improved, and technical support is provided for the detection of pea foot rot and the breeding of pea disease resistance.

The utility model discloses an animal and plant quarantine organism transfer device which comprises a transfer box, handles are installed at the two ends of the transfer box, lifting rods are rotatably installed at the two ends of the transfer box, a supporting mechanism is arranged in the transfer box, and the supporting mechanism can support and transfer animals and plants for quarantine. A supporting mechanism is arranged in a transfer box for animal and plant quarantine, small animals are stably supported through a transparent box and a supporting net plate, a bottom box is arranged below the supporting net plate, generated excrement and urine penetrate through meshes of the supporting net plate to fall into the bottom box, and organisms and excrement are separated; excrement and urine generated by organisms are intensively placed, so that pollution is reduced; and meanwhile, the first partition plate and the second partition plate are arranged in the transparent box to separate the internal space of the transparent box, so that the small animals can be independently isolated while being transferred, and the infection risk is reduced.

The application discloses a method for identifying Ectropis oblique and Ectropis grisescens, and belongs to the technical field of molecular biology. The application takes Ectropis oblique and Ectropis grisescens as research objects, systematically screens diagnostic different sites in the whole genome based on population genome resequencing data, and develops a nucleic gene molecular marker which can be used for rapid and accurate identification of close species. COI Compared with the mitochondrial markers in the prior art, the method has wider applicability and higher genetic variation sensitivity at the species and population levels. The molecular identification method established in the application provides reliable technical support for accurate identification of close pests and application of the close pests in plant quarantine and monitoring.

The invention provides a universal primer for rapidly detecting fusarium species and application of the universal primer. Specifically, the invention provides a primer for detecting fusarium species, and the primer is used for amplifying a fusarium specific section. Furthermore, the invention also provides an application of the universal primer of the fusarium species in detecting the fusarium species. By means of the universal primer of fusarium, different species in fusarium can be accurately distinguished, the universal primer can be applied to the field of plant quarantine, and rapid and accurate detection of fusarium species is achieved.

The invention discloses a pine wood nematode wood sample batch separation device, and relates to the technical field of plant quarantine, the pine wood nematode wood sample batch separation device comprises a first base and a partition plate; the second base is fixedly installed on the rear side face of the first base, the height of the first base is 0.25 m, the height of the second base is 0.6 m, the first base and the second base are combined in a stepped mode, the partition plates are evenly fixed in the first base and the second base, round holes are evenly formed in the top face of the first base and the top face of the second base, and the partition plates are arranged in the round holes. According to the device for separating the wood samples of the pine wood nematodes in batches, the plurality of glass funnels can be placed in the plurality of round holes, so that the samples of different batches can be treated at the same time, the operation is simple, the operation is convenient, and the efficiency is high. By rotating the screw rod, the pine wood nematode wood sample can be tightly pressed in the glass funnel by the pressing plate, so that the pine wood nematode wood sample is prevented from floating upwards to influence the experiment effect.

This invention relates to the field of tissue sectioning and sampling technology, and discloses a tissue sectioning device for plant quarantine. The device includes a hand-cranked drive assembly, a piston flow control assembly, an adjustment assembly, a feedback deceleration assembly, and a transfer assembly. The lifting block is linked to the piston block through the piston rod, and during the cutting stroke, it pulsarily presses liquid into the liquid flow chamber next to the slicing blade, generating a pulsed liquid flow synchronized with the slicing rhythm, realizing non-contact guiding and transferring of the wax strip. When cutting hard plant tissue, causing a sudden increase in cutting load, the load increases the pressure in the piston cylinder and pushes the adjustment assembly to switch the flow path. The feedback deceleration assembly converts the pressurized liquid into a normal clamping force of the push block on the turntable, thereby increasing the torque required for the rocker wheel to continue rotating and reducing the risk of impact damage during the cutting of hard tissue. This invention organically combines pulsed liquid flow transfer with hydraulic resistance increase and deceleration, providing a slicing solution with strong sample protection for plant quarantine.

The invention discloses a target gene, a primer group and a detection kit for detecting pea foot rot, and belongs to the technical field of plant quarantine. The invention creatively develops a rapid detection technology system based on a specific molecular marker aiming at the core pain points of long detection period (the detection period is longer than or equal to 20 days in a traditional method), difficulty in identification of sibling species (such as D.pinodes and A.pisi) and the like in quarantine identification of Didymellinosporium petiolatum (Didymellinosporium petiolatum (Didymellinosporium petiolatum (Didymellinosporium petiolatum (Didymellinosporium petiolatum (Didymellinosporium)). By screening a unique single copy gene sequence of D.pinodella and combining a PCR technology, a sample direct detection-target enrichment-signal amplification whole process system is established, pathogen detection can be completed within 8 hours, compared with a traditional method, the efficiency is improved by 96%, the efficiency is greatly improved, near-source species can be effectively distinguished, the customs clearance efficiency is greatly improved, and the method is suitable for large-scale popularization and application. The technical support is provided for the detection of the pea foot rot and the pea disease-resistant breeding.

The application relates to the technical field of plant quarantine, in particular to an imported grain weed rapid quarantine method based on DNA barcodes of compositae plants. The method aims to solve the problems of high missing detection rate and insufficient identification capacity when identifying highly degraded DNA samples. The scheme comprises the following steps: performing low-depth shotgun sequencing on total DNA of grain samples and filtering background sequences; extracting sequence and evolution features from short fragments by using a pre-trained double-channel deep feature extraction network; decoupling the fragment features into conservation, position coding and degradation features by using a decoupling-collaborative reconstruction network, and reconstructing a synthetic feature sequence representing a complete barcode; and finally comparing the sequence with a standard feature database, calculating an identification confidence, and outputting an identification conclusion. The application is especially suitable for rapid and accurate identification of compositae weed seeds with severely degraded DNA in imported grains.

The application discloses a kind of device and method of intelligent disinfection and sterilization applied to container, belong to container disinfection field.It includes device main body, disinfectant tank is arranged on the device main body, ultrasonic atomization generator, container insertion pipe, aerosol jet head hose, integrated gas detection air pipe, the aerosol jet head hose, integrated gas detection air pipe are all set in container insertion pipe, aerosol jet head hose is connected with disinfectant tank by ultrasonic atomization generator.The advantage of the application is that, compared with prior art: it can realize the disinfection and sterilization of animal and plant quarantine and health quarantine unmanned operation, reduce the error existing in personnel operation, realize operation process standardization, improve disinfection efficiency, and can avoid fumigating gas into air, ensure the safety of staff.

The invention discloses a PMA-qPCR-based activity detection method for Phaseolus vulgaris bacterial wilt germs, and belongs to the technical field of pathogen detection. According to the PMA-qPCR-based activity detection method for the bacterial wilt of phaseolus vulgaris disclosed by the invention, a group of high-specificity primers (CffFOR2 / CffREV4 / CF-P) are designed and are matched with an optimized PMA-qPCR detection system, so that live / dead bacterium mixtures in different proportions can be accurately distinguished, and the false positive rate is remarkably reduced in actual sample detection. The method not only provides a reliable technical means for bean seed health detection and plant quarantine, but also can be extensively applied to activity detection of other phytopathogens according to the technical principle and implementation scheme, and has important scientific value and wide application prospect.

The application discloses a port plant quarantine harmful organism image intelligent identification method and system, relates to the technical field of computer vision and image recognition, and comprises the following steps: acquiring an original image of a plant sample to be quarantined; performing multi-scale decomposition on the original image, calculating the consistency degree between the features of the images at each scale to obtain scale reliability, and performing weighted fusion according to the scale reliability to obtain multi-scale fusion features; matching the multi-scale fusion features with harmful organism reference features to obtain candidate species categories; positioning a discrimination region according to a discriminative feature description and extracting features of the discrimination region, sorting the candidate species categories according to the difference between the features of the discrimination region and the harmful organism reference features; determining feature response stability according to the response fluctuation degree of the multi-scale fusion features on the images at each scale, determining a harmful organism species, and outputting the harmful organism species. The application improves the accuracy and reliability of harmful organism identification.

The utility model relates to the technical field of plant quarantine, in particular to a nematode specimen manufacturing device. Comprising a primary dewatering box and a deep dewatering box which share a shell, the primary dewatering box comprises a primary dewatering bin with an upper cover and a first timing temperature controller connected with the primary dewatering bin, a dewatering liquid pool is placed on the lower portion in the primary dewatering bin, and a nematode box containing area is arranged on the upper portion in the primary dewatering bin; the deep dewatering box comprises a deep dewatering bin with an upper cover and a second timing temperature controller connected with the deep dewatering bin, a nematode box containing area is arranged in the deep dewatering bin, and the nematode box comprises a plurality of nematode vessels and vent holes formed in the peripheries of the nematode vessels. The nematode specimen processing device is reasonable in structure, ingenious in design and high in integration level, overcomes the defects that nematode bodies are difficult to dehydrate and time and labor are wasted in the nematode specimen manufacturing process, achieves one-stop processing operation in the nematode specimen manufacturing process, and improves the quarantine work efficiency and quality.

The present invention relates to the field of plant cultivation technology, and more specifically, to a light incubator for plant quarantine, comprising a base, a protective cover, a placement mechanism, and a conveying mechanism. The protective cover is disposed on the base; the placement mechanism is disposed on the base and within the protective cover, the placement mechanism comprising a rotating placement frame and a telescopic placement table movably connected to the rotating placement frame, the telescopic placement table having a cultivation container for carrying plants; and the conveying mechanism is disposed on one side of the rotating placement frame, the conveying mechanism comprising a conveying track and a movable arm for grasping items on the telescopic placement table. The present invention, through the conveying track and the movable arm, can individually remove a designated cultivation container, avoiding the impact on the growth of other plants caused by the large-scale opening of the protective cover, thereby solving the problem in the prior art that removing a single cultivation container may affect the cultivation of other plants.

This utility model belongs to the field of plant quarantine and discloses a plant quarantine device, including an outer box assembly for sealing and fumigating quarantine plants, and an inner box assembly disposed in the middle of the outer box assembly for holding the quarantine plants for rotary fumigation. A spraying assembly for reciprocating spraying of fumigant gas is installed on the top of the outer box assembly. The inner box assembly includes two symmetrically arranged rotating frames, with an inner box body between the two rotating frames. The inner box body adopts a horizontally arranged flip-top mesh cylindrical structure. A rotating shaft is disposed on the outer side of the rotating frames, and a servo motor is disposed at one end of the rotating shaft. The spraying assembly includes a translation component for reciprocating movement, with an atomizing nozzle installed below the translation component, and a gas delivery hose disposed behind the atomizing nozzle. This plant quarantine device combines the rotational design of the inner box assembly with the linear reciprocating motion of the spraying component to achieve multi-directional coverage of the fumigant in three-dimensional space, avoiding the dead zones problem of traditional static fumigation.

The invention relates to the technical field of plant quarantine and seed treatment, in particular to a crop seed treatment device and method for inspection and quarantine, the crop seed treatment device for inspection and quarantine comprises a totally-closed treatment cabin, and a cleaning unit, a sterilization unit and a drying unit which are arranged in sequence, and a rotary screen drum, an ultraviolet lamp array, an ozone generator and a hot air circulation system work cooperatively; the PLC control terminal is used for integrated regulation and control; according to the method, seed surface pollutant removal, pathogen efficient inactivation and activity maintenance can be synchronously completed in a closed environment, automatic recording of processing parameters and RFID batch traceability are supported, secondary pollution is effectively avoided, and the requirements of cross-border quarantine for operation closure, process controllability and data traceability are met.

The invention discloses a quarantine treatment method for phoenix heterothoracic beetles, belongs to the technical field of plant quarantine, and provides a quarantine treatment method for phoenix heterothoracic beetles harmful to palm plants (coconuts, phoenix and the like). The treatment method comprises the following steps: placing the phoenix dactylifera plants to be quarantine in an environment with the temperature of 0-5 DEG C, treating for 12-24 hours, spraying an acetamiprid medicament with the concentration of 10-20 mg / L, and standing for at least 24 hours. After the treatment is completed, the phoenix dactylicapnostoma on the phoenix seedlings is detected, and the result shows that the death rate of pests reaches 98% or above, and phoenix has good growth condition and no obvious damage.