11 results about "Plant quarantine" patented technology

The invention discloses an LAMP (loop-mediated isothermal amplification) primer group, a kit and a method for detecting four quarantine viruses and application of the LAMP primer group. Belongs to the technical field of molecular biology and plant quarantine. Aiming at conserved gene regions of bean pod mottle virus, tobacco ringspot virus, tomato ringspot virus and southern bean mosaic virus, a specific target sequence is screened through bioinformatics analysis, an LAMP primer group is designed, and the nucleotide sequence of the LAMP primer group is shown as SEQ ID NO.1-22. Furthermore, the screened primer group is combined with a micro-fluidic chip technology to construct a detection kit capable of synchronously detecting the four viruses, so that the specific identification of the four target viruses is realized. The kit has the advantages of rapidness, sensitivity, specificity, simplicity and convenience in operation and the like, the sensitivity can reach 10 <-10 > Copies / mu L, and the kit is suitable for high-throughput rapid screening of quarantine viruses in imported soybeans on port sites and has important significance in guaranteeing national biosafety.

This invention belongs to the field of plant quarantine technology and discloses an IoT-driven real-time monitoring and early warning device for plant quarantine pests. The on-site monitoring terminal collects multi-dimensional on-site data related to pests; the edge computing module performs real-time preprocessing, feature extraction, and preliminary anomaly detection on the on-site data; the IoT transmission module enables bidirectional data transmission between the edge computing module and the cloud-based early warning platform; and the cloud-based early warning platform performs in-depth analysis of the received data, pest identification and density assessment, and executes tiered early warning and data management. This invention provides comprehensive monitoring dimensions, enabling monitoring of all types of pests at all times: integrating multi-sensor detection units, image acquisition units, and trapping units, and combining pheromone, vibration, spore capture, and image acquisition methods, it achieves monitoring of various plant quarantine pests such as insects, fungi, and bacteria.

The utility model relates to the field of forestry plant quarantine samples, and discloses a forestry plant quarantine sample collecting device which comprises a handle, a fixed shell is arranged on one side of the handle, a movable groove is formed in one side of the fixed shell, a sliding block is connected in the movable groove in a sliding mode, and a detachable sampling cutter cylinder is fixedly connected to one side of the sliding block. The lower end of the sampling cutter cylinder is an edged end, the lower end of the fixed shell is fixedly connected with a supporting seat, and the upper end of the supporting seat is fixedly connected with a supporting boss matched with the sampling cutter cylinder; an air pipe is slidably inserted into one end, far away from the edged edge, of the sampling cutter cylinder, an air bag is fixedly connected to the upper end of the air pipe, a one-way air valve is arranged on the air bag, an adsorption block slidably connected into the sampling cutter cylinder is fixedly connected to the lower end of the air pipe, an air cavity is formed in the adsorption block, and an adsorption hole is formed in the bottom surface of the adsorption block. Compared with the prior art, the plant leaf slicing device has the advantages that plant leaves can be quickly sliced, and time and labor are saved.

The present application belongs to the technical field of plant quarantine, and particularly relates to a plant quarantine pest intelligent trapping device integrated with image recognition functions, which comprises a trapping main body module, an image acquisition and recognition module, an intelligent control module, a data transmission module and a power supply module, and each module is electrically connected with the intelligent control module through a line. The present application realizes integrated intelligent operation: the functions of trapping, image acquisition, real-time recognition, statistics, early warning and data uploading are integrated, the whole-process monitoring of quarantine pests can be completed without manual intervention, the cost of manual inspection and recognition is greatly reduced, and the work efficiency of quarantine is improved.

The invention provides an RPA (recombinase polymerase amplification) primer group, a kit and a method for detecting the alternaria brassicae of broccoli, which are used for detecting the alternaria brassicae of broccoli. The primer group comprises specific primers F1 / R1, F1 / R1 and F2 / R1, and the sizes of theoretical amplification products of the primer group are 204bp, 187bp and 189bp. Specific target genes are screened out through comparative genomics, RPA primers are designed, and a constant-temperature (39 DEG C) and rapid (20 minutes) detection method is established. The primer provided by the invention has strong specificity, is only effective to the brassica oleracea var. Brassicae, and has no cross reaction with sibling species; the sensitivity is high, the detection lower limit is 0.08 ng / mu L, and the sensitivity is 100 times higher than that of conventional PCR. The actual sample verifies that the detection accuracy reaches 100%. The method is easy and convenient to operate, rapid in detection and suitable for field early diagnosis, plant quarantine and disease monitoring of the pathogenic bacteria.

The invention discloses a pine wood nematode wood sample batch separation device, and relates to the technical field of plant quarantine, the pine wood nematode wood sample batch separation device comprises a first base and a partition plate; the second base is fixedly installed on the rear side face of the first base, the height of the first base is 0.25 m, the height of the second base is 0.6 m, the first base and the second base are combined in a stepped mode, the partition plates are evenly fixed in the first base and the second base, round holes are evenly formed in the top face of the first base and the top face of the second base, and the partition plates are arranged in the round holes. According to the device for separating the wood samples of the pine wood nematodes in batches, the plurality of glass funnels can be placed in the plurality of round holes, so that the samples of different batches can be treated at the same time, the operation is simple, the operation is convenient, and the efficiency is high. By rotating the screw rod, the pine wood nematode wood sample can be tightly pressed in the glass funnel by the pressing plate, so that the pine wood nematode wood sample is prevented from floating upwards to influence the experiment effect.

This invention relates to the field of tissue sectioning and sampling technology, and discloses a tissue sectioning device for plant quarantine. The device includes a hand-cranked drive assembly, a piston flow control assembly, an adjustment assembly, a feedback deceleration assembly, and a transfer assembly. The lifting block is linked to the piston block through the piston rod, and during the cutting stroke, it pulsarily presses liquid into the liquid flow chamber next to the slicing blade, generating a pulsed liquid flow synchronized with the slicing rhythm, realizing non-contact guiding and transferring of the wax strip. When cutting hard plant tissue, causing a sudden increase in cutting load, the load increases the pressure in the piston cylinder and pushes the adjustment assembly to switch the flow path. The feedback deceleration assembly converts the pressurized liquid into a normal clamping force of the push block on the turntable, thereby increasing the torque required for the rocker wheel to continue rotating and reducing the risk of impact damage during the cutting of hard tissue. This invention organically combines pulsed liquid flow transfer with hydraulic resistance increase and deceleration, providing a slicing solution with strong sample protection for plant quarantine.

The application relates to the technical field of plant quarantine, in particular to an imported grain weed rapid quarantine method based on DNA barcodes of compositae plants. The method aims to solve the problems of high missing detection rate and insufficient identification capacity when identifying highly degraded DNA samples. The scheme comprises the following steps: performing low-depth shotgun sequencing on total DNA of grain samples and filtering background sequences; extracting sequence and evolution features from short fragments by using a pre-trained double-channel deep feature extraction network; decoupling the fragment features into conservation, position coding and degradation features by using a decoupling-collaborative reconstruction network, and reconstructing a synthetic feature sequence representing a complete barcode; and finally comparing the sequence with a standard feature database, calculating an identification confidence, and outputting an identification conclusion. The application is especially suitable for rapid and accurate identification of compositae weed seeds with severely degraded DNA in imported grains.

This utility model belongs to the field of plant quarantine and discloses a plant quarantine device, including an outer box assembly for sealing and fumigating quarantine plants, and an inner box assembly disposed in the middle of the outer box assembly for holding the quarantine plants for rotary fumigation. A spraying assembly for reciprocating spraying of fumigant gas is installed on the top of the outer box assembly. The inner box assembly includes two symmetrically arranged rotating frames, with an inner box body between the two rotating frames. The inner box body adopts a horizontally arranged flip-top mesh cylindrical structure. A rotating shaft is disposed on the outer side of the rotating frames, and a servo motor is disposed at one end of the rotating shaft. The spraying assembly includes a translation component for reciprocating movement, with an atomizing nozzle installed below the translation component, and a gas delivery hose disposed behind the atomizing nozzle. This plant quarantine device combines the rotational design of the inner box assembly with the linear reciprocating motion of the spraying component to achieve multi-directional coverage of the fumigant in three-dimensional space, avoiding the dead zones problem of traditional static fumigation.

The invention relates to the technical field of plant quarantine and seed treatment, in particular to a crop seed treatment device and method for inspection and quarantine, the crop seed treatment device for inspection and quarantine comprises a totally-closed treatment cabin, and a cleaning unit, a sterilization unit and a drying unit which are arranged in sequence, and a rotary screen drum, an ultraviolet lamp array, an ozone generator and a hot air circulation system work cooperatively; the PLC control terminal is used for integrated regulation and control; according to the method, seed surface pollutant removal, pathogen efficient inactivation and activity maintenance can be synchronously completed in a closed environment, automatic recording of processing parameters and RFID batch traceability are supported, secondary pollution is effectively avoided, and the requirements of cross-border quarantine for operation closure, process controllability and data traceability are met.

The invention belongs to the technical field of biological detection and animal and plant quarantine, and particularly relates to a differential primer and probe-based RPA / RAA-LFS and TaqMan qPCR double-path rapid identification system, a primer and a kit. According to the invention, a mitochondrial ND2 gene segment is taken as a detection target, and a specific primer pair and a specific site are adopted to introduce a locked nucleic acid modified structure probe, so that a quantitative path of RPA field detection is realized; meanwhile, a laboratory quantitative detection path based on real-time fluorescent quantitative PCR (qPCR) is established. The primer and the probe obtained through screening can effectively distinguish sibling or non-target species such as the gossypodium beetle and the red material gossypodium beetle, the polypodium spp. And the pectoralis fuscopoda. The system realizes unification of RPA-LFS field signal quantification and laboratory double-path detection, provides an efficient and accurate molecular detection technology for port quarantine, forestry pest monitoring and invasive species emergency prevention and control, and has significant popularization and application values.