243 results about "Bioinformatics analysis" patented technology

The invention discloses a crested ibis habitat ecological quality trend analysis method and system, and relates to ecological quality monitoring. The method comprises the following steps: S1, constructing an intelligent sensing network, synchronously obtaining multi-source data of a habitat, identifying activity events of crested ibis, and generating a multi-dimensional habitat parameter table; s2, collecting environmental samples, and generating a microbial functional gene abundance matrix through metagenome sequencing and bioinformatics analysis; s3, taking the activity events of the crested ibis as behavior tags, and generating habitat function health indexes by coupling the parameter table and the matrix training machine learning prediction model; s4, performing spatial interpolation and trend analysis based on the habitat function health index to generate an ecological quality space-time evolution graph; and S5, based on the ecological quality space-time evolution graph, performing quantitative analysis by using a spatial differentiation statistical model, and generating a trend analysis report. By fusing multi-source data, real-time dynamic evaluation of habitat ecological quality and quantitative analysis of driving factors are realized, and a direct decision basis is provided for accurate protection.

The invention relates to the technical field of nucleic acid detection, in particular to a product and a method for detecting bifidobacterium longum subsp. Longum 6-1. Bioinformatics analysis is carried out based on a whole genome sequence of the bifidobacterium longum subspecies longum 6-1, the primer pair and the primer probe combination are obtained through optimization, and the primer pair and the primer probe combination have good specificity and sensitivity in the aspect of detecting the bifidobacterium longum subspecies longum 6-1; the bifidobacterium longum subsp. Longum 6-1 and other strains (including other same subsp. Longum strains, other same strains and other strains) can be quickly and accurately distinguished, and the strain level quantitative detection of the strain in a complex biological sample (such as excrement) can be realized.

The invention belongs to the technical field of tumor molecular diagnosis and biological information analysis, and relates to a high-throughput sequencing method and system for monitoring acute lymphocytic leukemia (MRD). Through targeted sequencing with a unique molecular identifier and / or a double-chain tag, error modeling based on a background noise spectrum and statistics / machine learning pseudo variation filtering, ultra-deep accurate detection of IG / TCR cloning and related gene low-frequency variation is realized. And an artificial intelligence recurrence risk prediction model is established by combining a time sequence MRD index, cloning diversity and clinical information, and a structured clinical report is output and docked with LIS / HIS. According to the method, the sensitivity and the specificity of ALL minimal residual disease detection can be remarkably improved, dynamic evaluation on leukemia cloning evolution and recurrence risks is realized, and a reliable basis is provided for individualized treatment decision and long-term follow-up visit.

The invention relates to the technical field of oral medicine and biological information analysis, in particular to an evaluation system for oral microbiological analysis and decayed tooth risk prediction. The system comprises a sample collection module, a microorganism detection module, a data preprocessing module, a microorganism population dynamic analysis module, a multi-dimensional feature extraction module, a decayed tooth risk assessment module and a personalized suggestion generation module. By constructing microbial population evolution analysis based on a Lotka-Volterra competitive model, diversity dynamic change analysis based on Shannon entropy and evolution trend prediction based on a Lyapunov stability theory, the system deeply reveals a dynamic evolution law of an oral micro-ecosystem, and a multi-dimensional feature adaptive weighting mechanism based on mutual information is adopted, so that the dynamic evolution law of the oral micro-ecosystem is improved. Personalized accurate risk assessment is realized, a causal reasoning theory is introduced to generate personalized intervention suggestions with causal support, and the accuracy and the prevention effect of dental caries risk prediction are remarkably improved.

The invention relates to the technical field of plant genetic engineering, and particularly provides cloning of a GbGELP25D gene of gossypium barbadense and application of the GbGELP25D gene of gossypium barbadense. According to the invention, the GbGELP25D gene with a full length of 1092 bp is cloned from the sea island cotton variety Xinhai No. 7 for the first time, and the gene encodes a secretory lipase protein with a signal peptide and is positioned in an extracellular gap. Through bioinformatics analysis, phylogenetic classification, protein structure modeling and signal peptide function verification, it is clear that the gene belongs to a plant GELP family. Furthermore, a virus-induced gene silencing technology is utilized to prove that the silent GbGELP25D can obviously enhance the resistance of cotton to verticillium wilt, and the mechanism of the silent GbGELP25D is closely related to activation of ethylene synthesis and signal channels and induction of expression of disease-resistant related genes. The resistance gene provided by the invention enriches gene resources of cotton verticillium wilt resistance breeding, and has important theoretical significance and application value.

The invention discloses a detection system and method for identifying mycobacteria and drug resistance genes of the mycobacteria based on nanopore sequencing, and relates to the field of biological medicine, the detection system comprises a specific targeted enrichment module, a nanopore sequencing module and a biological information analysis module; the specific targeted enrichment module comprises a probe combination, and the probe combination covers a mycobacterium tuberculosis complex conservative identification gene, species-specific genes of common nontuberculous mycobacteria and mycobacterium leprosy, and full-length or partial sequences of drug resistance related genes in a targeted manner; by utilizing the characteristics of nanopore length reading length and real-time sequencing and a tuberculosis specific targeted enrichment strategy, accurate identification of a mycobacterium tuberculosis complex group, synchronous typing of 42 mycobacteria, analysis of 24 drug-resistant genes, and efficient detection of structural variation and low-abundance heterogeneity drug-resistant mutation are realized, the detection period is shortened, the detection cost is reduced, and the detection efficiency is improved. And a comprehensive and reliable technical basis is provided for accurate diagnosis and treatment of mycobacterium infection.

The present application relates to the field of biological medicine, and particularly relates to biomarkers for migraine diagnosis, kits and application thereof. The biomarkers related to migraine disease according to the present application include COL4A2, and also include any one or a combination of multiple of MMP-14, LCAT, ADAMTS13 and CPXM2. The present application is based on proteomic data of clinical healthy controls and migraine patients, and uses bioinformatics analysis and machine learning methods to deeply mine protein combinations with the most early warning value for migraine disease diagnosis, and to verify in a clinical cohort with expanded samples, to provide a good prospect for clinical transformation of newly discovered biomarkers, and also to lay a foundation for subsequent mechanism research.

The application belongs to the technical field of molecular plant pathology, and particularly relates to a peanut disease-resistant gene AhNHL24 and application thereof. Through whole genome identification and bioinformatics analysis, 45 NHL family genes (AhNHL) are identified in peanuts, and gene expression analysis shows that most of the AhNHL genes can respond to the induction of biological stress and abiotic stress. Gene disease resistance identification finds that transient overexpression of AhNHL14, AhNHL24 and AhNHL31 can significantly improve the resistance of tobacco and peanuts to Pseudomonas solanacearum, and the disease resistance of AhNHL24 is the strongest. Through transgenic tobacco heterologous overexpression of AhNHL24 and inoculation experiments, it is shown that the peanut and tobacco plants overexpressing AhNHL24 not only have significantly enhanced resistance to Pseudomonas solanacearum, but also have significantly enhanced resistance to Sclerotium rofsii. The application provides an important basis for the cultivation of plant (peanut and tobacco, etc.) broad-spectrum disease-resistant varieties.

The invention discloses a method and system for synchronously detecting host chromatin openness and in-vivo microbiome based on transposase, and belongs to the technical field of biological sequencing data analysis. According to the method, transposase is used for selectively fragmenting an open chromatin region of a host, and a microbial genome is almost randomly cut, so that synchronous enrichment of host and microbial DNA is realized; after high-throughput sequencing library construction and double-end sequencing, sequencing data is split into host source and non-host source reads through bioinformatics analysis, host chromatin state and microorganism composition are analyzed respectively, and a microorganism-host epigenetic regulation network is constructed; the invention further provides a matched DNA sequencing library and an analysis system, multi-scene research of infectious diseases, intestinal microecology, tumor microenvironment and the like is supported, a public database can be reanalyzed, and potential microbial interaction signals are mined. According to the method, the host-microorganism interaction research efficiency is remarkably improved, and a high-sensitivity and integrated technical scheme is provided for epigenetic regulation mechanism analysis.

The invention relates to a method for evaluating and positioning nucleic acid pollution of a nucleic acid extraction kit, which comprises the following steps: 1) taking nucleic acid-free water as a negative sample, and performing nucleic acid extraction on the nucleic acid-free water by using the nucleic acid extraction kit according to nucleic acid extraction steps in the kit, the obtained nucleic acid is presumed as the polluted nucleic acid in the nucleic acid extraction kit; 2) amplifying polluted nucleic acid, purifying a PCR product, enriching double-chain cDNA, and determining the concentration of the double-chain cDNA so as to perform quantification, sample mixing and quality control treatment before library construction; (3) carrying out high-throughput deep sequencing by adopting a platform of the Illumina company, and then carrying out bioinformatics analysis to obtain classification information and gene sequences of polluted nucleic acid; and 4) designing a specific primer based on the gene sequence of the polluted nucleic acid, and carrying out pollution evaluation and positioning verification on each component of the nucleic acid extraction kit through qPCR (Quantitative Polymerase Chain Reaction).

The invention relates to the technical field of biological detection, and discloses a metabonomics-based caenorhabditis elegans compound pollutant exposure evaluation method which comprises the following specific steps: S1, constructing a compound pollutant exposure system; s2, carrying out nematode culture and exposure treatment; s3, extracting in-vivo metabolites of the nematodes; s4, carrying out metabonomics analysis; s5, carrying out bioinformatics analysis; and S6, verifying the combined toxicity effect. By establishing the metabonomics-based caenorhabditis elegans compound pollutant exposure evaluation method, the limitation of a traditional single pollutant evaluation system is broken through, and systematic and high-sensitivity detection of multi-pollutant combined toxicity is realized; in addition, by constructing a standardized composite exposure system and an efficient metabolite extraction process and combining multi-dimensional mass spectrum data and bioinformatics modeling, a synergistic, antagonistic or additive effect mechanism among pollutants is deeply analyzed from the molecular level.

The invention discloses an LAMP (loop-mediated isothermal amplification) primer group, a kit and a method for detecting four quarantine viruses and application of the LAMP primer group. Belongs to the technical field of molecular biology and plant quarantine. Aiming at conserved gene regions of bean pod mottle virus, tobacco ringspot virus, tomato ringspot virus and southern bean mosaic virus, a specific target sequence is screened through bioinformatics analysis, an LAMP primer group is designed, and the nucleotide sequence of the LAMP primer group is shown as SEQ ID NO.1-22. Furthermore, the screened primer group is combined with a micro-fluidic chip technology to construct a detection kit capable of synchronously detecting the four viruses, so that the specific identification of the four target viruses is realized. The kit has the advantages of rapidness, sensitivity, specificity, simplicity and convenience in operation and the like, the sensitivity can reach 10 <-10 > Copies / mu L, and the kit is suitable for high-throughput rapid screening of quarantine viruses in imported soybeans on port sites and has important significance in guaranteeing national biosafety.

The application belongs to the technical field of biotechnology and particularly relates to a multifunctional bioactive peptide based on highland barley wine lees protein and a preparation method thereof, wherein the amino acid sequence of the bioactive peptide is shown as SEQ ID NO:1. The bioactive peptide has small molecular weight, is easy to absorb, can inhibit tyrosinase activity and efficiently scavenge free radicals, and has the advantages of non-toxicity, non-carcinogenicity, high hydrophilicity, long half-life and the like through bioinformatics analysis prediction, and can be applied to the development of drugs for treating skin pigmentation and oxidative stress related diseases or daily chemicals for whitening, anti-aging, antioxidant and the like, and has a wide application prospect.

The invention belongs to the technical field of biological medicine and molecular biology, and particularly relates to application of MIGA2 serving as a target spot in screening or preparing a medicine for treating colorectal cancer. According to the invention, the correlation between MIGA2 and colorectal cancer progress is analyzed through large-queue bioinformatics; the influence of MIGA2 on the proliferation, migration and invasion ability of colorectal cancer cells is analyzed from an in-vitro cell model; analyzing a key molecular mechanism of MIGA2 playing a cancer inhibition function; the influence of MIGA2 on colorectal cancer tumor growth is analyzed in an in-vivo mouse model, the influence of MIGA2 mediated mitochondrial-endoplasmic reticulum contact on colorectal cancer tumor growth is analyzed, and it is verified in multiple dimensions that MIGA2 can become an effective treatment target of colorectal cancer.

The invention discloses a three-channel bionic bone organ chip and a method thereof.The three-channel bionic bone organ chip comprises a three-channel model and a carrier, the three-channel model comprises a blood vessel channel in the middle and bone channels located on the two sides of the blood vessel channel, the width of the blood vessel channel is 500 micrometers, the width of the bone channels is 1000 micrometers, and the width of the carrier is 100 micrometers. The two sides of the blood vessel channel are separated from the bone channels on the two sides through long barriers respectively. The carrier is used for bearing the three-channel model. According to the invention, a novel bone organ chip (Bone Orga-on-a-Chip, BOC) is constructed, which is convenient for simulating an OP microenvironment induced by glucocorticoid and the influence of mechanical mechanics on treatment of OP by MSCs. On the basis, a candidate gene TPD52L1 is further analyzed and screened through bioinformatics, the candidate gene TPD52L1 is remarkably up-regulated in MSCs induced osteogenic differentiation, and the effect of the gene in MSCs osteogenic adipogenic fate choice is discussed.

The invention belongs to the technical field of biological medicine, and particularly relates to a biomarker related to acetylation of a K147 site of histone H1 and application of the biomarker in liver cancer treatment. The invention relates to a biomarker related to acetylation of a K147 site of histone H1. According to the biomarker, the histone H1 is subjected to acetylation modification at the K147 site. Bioinformatics analysis shows that the high expression state of acetyltransferase ELP3 is closely related to the occurrence and development of hepatocellular carcinoma, ELP3 can directly regulate and control acetylation modification of histone H1, then the core catalytic site of ELP3 is verified through site mutation, and the K147 acetylation site located on the histone H1 is precisely determined. Test results show that intervention of the ELP3-H1-K147 pathway can significantly inhibit proliferation and migration ability of liver cancer cells, and can provide research and development direction and theoretical support for development of novel liver cancer treatment drugs.

The invention provides a signal peptide category and cleavage site prediction method and system based on multi-modal characteristics, and belongs to the technical field of biological information analysis. The method comprises the following steps: acquiring an amino acid sequence of a signal peptide sample, and acquiring three-dimensional structure data of the signal peptide by utilizing a protein structure prediction model; obtaining sequence modal input data of the amino acid sequence of the signal peptide, and constructing a structural diagram to obtain structural modal input data; inputting the sequence modal input data into a sequence encoder and a protein language model, and extracting sequence features; inputting the structural modal input data into a structural encoder, and extracting structural features through graph convolution operation; carrying out fusion processing on the sequence features and the structural features to obtain multi-modal feature representation; and outputting a category prediction result and a cleavage site prediction result of the signal peptide through a prediction module. According to the invention, through fusion of the sequence and the structure information, the accuracy of signal peptide prediction and the recognition capability of minority class samples are improved.

The invention provides a fish creatine kinase sensitization and immune cross reactivity analysis method which comprises the following steps: preparing a fish muscle water-soluble protein crude extract, separating 40-45kDa protein components in the fish muscle crude extract through SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis), and screening a target sensitization protein combined with IgE / IgG through immunoblotting; the method comprises the following steps: extracting total RNA of grass carp and synthesizing cDNA, designing a creatine kinase specific primer for amplification to obtain a creatine kinase gene sequence, and carrying out bioinformatics analysis after sequencing verification; the method comprises the following steps: cloning a creatine kinase gene to an expression vector, transforming escherichia coli BL21, performing IPTG induced expression, and performing urea gradient dialysis renaturation on an inclusion body to obtain the recombinant grass carp creatine kinase. Detecting the IgE binding activity of the recombinant grass carp creatine kinase and the serum of the allergic patient through dot hybridization; the IgG cross reactivity of the recombinant grass carp creatine kinase and the blue crab arginine kinase is verified through indirect ELISA; the IgE cross reaction degree of the recombined grass carp creatine kinase and the blue crab arginine kinase is quantified through inhibitory dot hybridization.

The application belongs to the technical field of biotechnology, and particularly relates to application of albumin polypeptide gel beads in a product for treating or preventing sarcopenia. Egg white protein is used as raw material to extract protein, and albumin dipeptide-based peptidase-IV inhibitory peptide is prepared through a biological enzymolysis method. Bioinformatics analysis is used to screen out the inhibitory peptide TPYMFP, and the IC50 value of the inhibitory peptide TPYMFP for dipeptide-based peptidase-IV reaches 3.12 ± 0.49 mmol / L. Further, the optimal preparation conditions of sodium alginate SA and carboxymethyl chitosan CMCS-based gel beads CMCS / SA / TPYMFP are explored. When the SA concentration is 1.5% (w / v), the CMCS concentration is 0.5% (w / v), and the CaCl2 concentration is 1% (w / v), the polypeptide encapsulation rate is increased to 98.26% ± 0.25%. It is verified that the albumin polypeptide gel beads have good pH response characteristics, and efficient protection and intestinal target delivery of the sarcopenia inhibitory peptide are realized. The albumin polypeptide gel beads are applied to preparation of a product for treating or preventing sarcopenia, or in preparation of a related product for increasing muscle mass and strength.

This invention belongs to the field of biotechnology and discloses a nucleic acid aptamer that specifically recognizes placental-specific protein 1 and its uses. The nucleotide sequence of the nucleic acid aptamer includes any one of the sequences shown in SEQ ID No. 1-57. This invention employs X-aptamer screening technology, combined with high-throughput sequencing technology and bioinformatics analysis, to reduce the number of screening rounds and obtain candidate nucleic acid aptamers. Further analysis of their affinity and specificity yields a nucleic acid aptamer that specifically recognizes placental-specific protein 1. The nucleic acid aptamer of this invention has the characteristics of high affinity and specificity, good stability, convenient synthesis, and easy labeling of functional groups. It can be used for the detection or binding of placental-specific protein 1, the diagnosis of placental-specific protein 1-related diseases, and the preparation of products for prognosis.

The application belongs to the field of marine biotechnology, and specifically discloses a preparation method of a long oyster insulin-like peptide receptor (ILPR) polyclonal antibody and application thereof. The method is characterized by the following steps: specific antigen epitopes of the long oyster ILPR protein are screened through bioinformatics analysis, a pET32a-ILPR recombinant plasmid is constructed and induced to express in Escherichia coli; the purified recombinant protein is used as an immunogen to immunize New Zealand white rabbits, and rabbit antisera are collected after four times of booster immunization; specific polyclonal antibodies are purified by using antigen affinity chromatography technology, and can specifically recognize the ILPR protein in long oyster tissues. The antibody prepared by the application has high specificity and sensitivity, and provides an important biological tool for studying the insulin signal transduction pathway, growth regulation and energy metabolism mechanism of long oysters.

The invention aims to provide a peptide for accelerating fat metabolism and application thereof, the peptide is prepared into a product convenient to use, and the amino acid sequence of the peptide is MVHSYSETSD. The peptide is obtained through peptide library screening, bioinformatics analysis screening and fat cell decomposition experiment verification, and it is verified that the active peptide obtained through screening has the effects of accelerating fat metabolism, inhibiting fat accumulation, reducing blood fat and body fat content and reducing body weight; the insulin sensitivity and the glucose tolerance are improved, and the prevention and treatment effects on obesity and complications thereof are realized.

The application aims to provide a peptide for accelerating fat metabolism and application thereof, and to prepare the peptide into a product convenient to use, and the amino acid sequence of the peptide is MVHSYSETSD. The peptide is obtained through screening of a peptide library, screening through bioinformatics analysis, and verification through fat cell decomposition experiment, and the application verifies that the active peptide screened has the effect of accelerating fat metabolism, inhibiting fat accumulation, reducing blood fat and body fat content, reducing body weight, improving insulin sensitivity and glucose tolerance, and realizing the prevention and treatment effect on obesity and its complications.

This invention discloses a Panax notoginseng ethylene response factor gene. PnERF120 Its nucleotide sequence is shown in SEQ ID NO:1, and the encoded amino acid sequence is shown in SEQ ID NO:2. PnERF120 The gene originates from Panax notoginseng, and the encoded protein sequence contains a typical conserved domain of the ERF transcription factor. This invention demonstrates, through gene cloning, bioinformatics analysis, expression pattern analysis, subcellular localization, overexpression, and other techniques and methods, that... PnERF120 This invention can be used to improve the resistance of Panax notoginseng to Panax notoginseng A virus, and also to improve the resistance of tobacco to tobacco mosaic virus. This invention provides new functional genes and application pathways for antiviral breeding and disease-resistant materials of Panax notoginseng and other plants.

This invention discloses a specific molecular marker for identifying eggplant Saet CMS cytoplasmic male sterility lines and its application. The molecular marker, Saet218b, was selected by comparing the mitochondrial genomes of the eggplant Saet CMS cytoplasmic male sterility line and its maintainer line. Through bioinformatics analysis, the candidate specific gene for Saet CMS male sterility, orf218b, was obtained, and its nucleotide sequence is shown in SEQ ID NO: 1. Based on this sequence information, the molecular marker Saet218b for identifying eggplant Saet CMS cytoplasmic male sterility lines was successfully designed. The molecular marker of this invention can be used to identify eggplant Saet CMS cytoplasmic male sterility lines efficiently and with a simple procedure. The results can be used for marker-assisted breeding of eggplant Saet-derived cytoplasmic male sterility lines, improving breeding efficiency. It also provides technical support and theoretical basis for the identification of eggplant specific cytoplasmic germplasm.

The invention belongs to the technical field of bioengineering, and particularly relates to a preparation method and application of an N protein polyclonal antibody of a horse coronavirus. The polyclonal antibody is prepared by truncation expression of the horse coronavirus N protein, and a material is provided for research on inhibition of host innate immune mechanism by the horse coronavirus N protein. Prokaryotic expression is carried out on the N protein of the equine coronavirus, the polyclonal antibody is prepared, and a method and a material are provided for molecular research and mechanism research of the equine coronavirus. According to the invention, in combination with bioinformatics analysis of an N protein of the horse coronavirus, a pGEX-6p-N3 recombinant plasmid is constructed, and prokaryotic expression is carried out, so that a purified recombinant protein is obtained. After a mouse is immunized by the recombinant protein, a polyclonal antibody is obtained, an indirect ELISA method detects that the polyclonal antibody has good titer, Western Blot detection has good reactivity, and the polyclonal antibody can be applied to an indirect immunofluorescence test.

The invention belongs to the technical field of biological medicines, and particularly relates to application of an ITGA6 inhibitor in preparation of a medicine for treating uveal melanoma. By integrating bioinformatics analysis of databases of TCGA, GTEx, CPTAC and the like, the invention systematically reveals that the ITGA6 has high specific expression in uveal melanoma and is significantly related to poor prognosis of a patient for the first time. In-vitro experiments prove that by knocking down ITGA6, proliferation, migration, invasion and colony forming ability of UVM cells can be effectively inhibited. According to the invention, small molecular compounds such as 4.5-diphenyl-1H-imidazole, MS-275, W-13 and the like and siRNA (small interfering Ribonucleic Acid) targeting ITGA6 are screened out to be used as effective inhibitors, and specific drug dosage forms such as intraocular injection and the like are provided. In addition, the ITGA6 inhibitor can be used in combination with a PD-1 / PD-L1 inhibitor, a CTLA-4 inhibitor or a chemotherapeutic drug to generate a synergistic anti-tumor effect. Mechanism research shows that ITGA6 promotes UVM progress by regulating a signal channel and a tumor immune microenvironment. The invention provides a new targeted treatment strategy for uveal melanoma, and has important clinical application value.

The application belongs to the field of tumor molecular diagnosis and targeted therapy, and specifically finds lncRNA RP11-499F3.2 which is significantly highly expressed in head and neck cancer through bioinformatics analysis of TCGA data; it is verified through clinical samples that the expression level of RP11-499F3.2 in head and neck squamous cell carcinoma is significantly higher than that in paracancerous tissues, and is closely related to the clinical prognosis of patients with head and neck squamous cell carcinoma; it is found through functional experiments that high expression of RP11-499F3.2 can promote the in-vitro proliferation, migration and invasion of head and neck squamous cell carcinoma cells; meanwhile, it is also found that RP11-499F3.2 can promote the drug resistance of head and neck squamous cell carcinoma cells to cetuximab; the disclosed lncRNA RP11-499F3.2 helps to reveal the new pathogenesis of head and neck cancer, provides a new tumor marker for the prognosis monitoring of head and neck cancer, and provides a new idea for the clinical treatment of head and neck cancer.

The invention relates to the cross technical field of biomedical technology, bioinformatics and molecular biology, and discloses a medicinal plant exosome anti-inflammatory regeneration mechanism identification method, which comprises the following steps: separating a high-purity exosome from medicinal plant tissue, and characterizing the high-purity exosome to be qualified; carrying out transcriptome and metabolome data acquisition on qualified samples; inputting the standardized data into a bioinformatics analysis process, integrating multiple omics data to identify a key regulation and control module and a path, and proposing a mechanism hypothesis; and finally, designing in vitro and in vivo function verification experiments based on a prediction mechanism. According to the method disclosed by the invention, through in vivo and in vitro linkage verification, the regulation effect of the exosome on inflammatory factors and pathway gene expression is verified at a cellular level in vitro so as to clarify a molecular mechanism; the promotion effect of the exosome on the wound healing rate and the tissue regeneration physiological effect is verified by using an animal model in vivo to form a complete evidence chain, the repeatability is ensured by strictly standardizing technical details, and a scientific basis is provided for development and application of the medicinal plant exosome.

The application provides application of circ-0007527 as a target in screening of drugs for treating peritoneal dialysis related peritoneal fibrosis. The application also provides application of an inhibitor of circ-0007527 in preparation of a drug for treating peritoneal dialysis related peritoneal fibrosis. In a mouse peritoneal fibrosis model induced by high glucose peritoneal dialysis solution, circRNA expressed in peritoneal tissues is screened, and after bioinformatics analysis, it is found that the differential expression amount of circ-0007527 in the fibrotic peritoneal tissue is the highest, and the high expression of circ-0007527 in the fibrotic peritoneal tissue is verified by real-time fluorescent quantitative PCR. Further in-vitro cell experiments find that down-regulation of the expression of circ-0007527 can significantly inhibit peritoneal mesothelial cell phenotype transformation, proliferation and apoptosis, and thus reduce peritoneal injury and delay peritoneal fibrosis progression.