Blood metagenome sequencing data analysis method and device and application thereof

Abstract

The invention discloses a blood metagenome sequencing data analysis method and device and an application thereof. The blood metagenome sequencing data analysis method comprises a data quality controlstatistics and splitting step, a humanized sequence removal step, a plasmid sequence removal and internal reference comparison step, a pathogen genome sequence library comparison step and a pathogen parameter annotation step. The invention provides a blood flow infection detection device based on a blood metagenome sequencing data analysis method. According to the method and device provided by theinvention, the free nucleic acid sequences in blood are detected through a metagenome sequencing method, a bioinformatics analysis method is combined, more than 8,000 pathogenic microorganisms such as bacteria, viruses, fungi and parasites possibly existing in a blood flow infection patient body are detected at a time, and a detection result can be obtained within 24 hours at the soonest. The speed, the sensitivity, the accuracy and the efficiency of blood flow infection detection are greatly improved.

Description

technical field [0001] The present application relates to the field of bloodstream infection detection, in particular to an analysis method, device and application of blood metagenomic sequencing data. Background technique [0002] Bloodstream infection is a serious systemic infectious disease, including sepsis and bacteremia, with high clinical morbidity and high mortality. The traditional bloodstream infection detection method is blood culture, that is, blood is collected from the patient and cultured in bottles containing culture medium to determine the microorganisms causing the patient's infection. Usually, after the blood culture is found to be positive, further isolation and identification of the pathogen is required to determine the infected pathogen and guide medication. Blood cultures remain the gold standard for diagnosing bloodstream infections. [0003] However, blood culture has the following disadvantages: 1) the amount of blood required is large, generally ...

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Problems solved by technology

[0003]However, blood culture has the following disadvantages: 1) the amount of blood collection required is large, generally 20-30ml for adults, and more than 2ml for infants; 2) the cycle of blood culture is relatively long It takes at least 3-4 days; 3) Blood culture can only be cultured for some bacteria or fungi, and the culture results cannot be obtained for viruses and some bacteria that are difficult to culture; 4) The positive rate of blood culture is low, only 30 %-40% of blood culture results are positive, that is, only 30-40% of bloodstream infections can be detected by culturing pathogenic bacteria; 5) The false positive rate of blood culture is high, although some samples can be obtained blood Positive culture results, but due to incomplete skin disinfection or irregular blood collection procedures often cause false positive cultures, affecting clinical judgment

At present, metagenomic sequencing is basically only applied to the study of environmental microbial communities, and there are no related studies and reports on its use in blood testing

Moreover, the complexity of bloodstream infection detection objects is different from that of environmental microbial communities; therefore, metagenomic sequencing directly used for the detection of environmental microbial communities cannot be directly applied to the detection and analysis of bloodstream infections

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