31 results about "Pseudomonas denitrificans" patented technology

The present invention relates to a promoter system inducing expression of 3-hydroxypropionic acid (3-HP) and a method of biologically producing 3-HP using the same. To improve production of 3-HP in a biological manner, continuous synthesis of new enzymes having enzyme activity is necessary. As a result of screening 3-HP reactive transcription regulators and 3-HP reactive promoters from several microorganisms including Pseudomonas denitrificans, it was confirmed that the transcriptions regulations and promoters are composed of LysR proteins and particular gene nucleotide sequences binding to the LysR proteins. Therefore, the 3-HP inducible system is expected to be effectively used to regulate 3-HP metabolic pathways.

This invention consists of a consortium of plant-growth promoting microorganisms including Pseudomonas stutzeri, Pseudomonas denitrificans, Pseudomonas resinovorans, Pseudomonas brassicacearum, Pseudomonas fluorescens, Shimwellia blattae and Klebsiella oxytoca. The bacterial consortium along with a vehicle suitable for agricultural application, form a biofertilizer useful to enhance agricultural yield when applied to cultivation plants. The biofertilizer described present a long shelf life and preserve a high cell density along the time.

The invention relates to a culture medium for producing vitamin B12 by fermenting pseudomonas denitrificans and a fermentation method thereof, and a method for producing vitamin B12 by the same. The culture medium comprises a primary seed culture medium, a secondary seed culture medium and a fermentation culture medium all of which contain artificial molasses composed of maltose, thiamine, riboflavin and biotin. By substituting the artificial molasses composed of maltose, thiamine, riboflavin and biotin for beet molasses and optimizing the formula of the culture medium, the invention solves the problem that the fluctuation range of fermentation titer is large because the quality of beet molasses is unstable, and acquires a method for stably and effectively producing vitamin B12. The invention saves the doses of raw and auxiliary materials, lowers the comprehensive cost and reduces the waste water discharge.

The invention relates to a composite material for removing nitrogen and phosphorus in wastewater, which comprises composite particles containing Pseudomonas denitrificans ATCC 13867 and Acinetobacter johnsonii ATCC 17909; the composite particles include the following components in parts by weight: 25-45 parts of iron tailings, 15-30 parts of steel slag powder, 15-30 parts of pine bark powder and 10-15 parts of clay. The present invention also relates to a method for treating wastewater by using the above-mentioned composite material. The invention utilizes the composite material processed by solid waste iron tailings, steel slag, etc. to simultaneously remove nitrogen and phosphorus in wastewater, and the processed composite material can be used to make organic fertilizer or seedling substrate, and simultaneously realize wastewater treatment and solid waste The dual purpose of resourcing.

The present invention relates to the field of soil remediation, and discloses an in-situ remediation preparation for quinclorac contaminated soil, the in-situ remediation preparation for quinclorac contaminated soil comprises fungi and / or bacteria with quinclorac degrading capability; the fungi are at least one of Aspergillus niger, rhizomucor variabilis and cunninghamella bertholletiae, and the bacteria are at least one of Pseudomonas denitrificans, Burkholderia caribensis and Serratia marcescens. The in-situ remediation preparation is used for in-situ bioremediation of the quinclorac contaminated soil, the problems that photochemical degradation method is vulnerable to the restriction of light conditions and secondary chemical reagent pollution of farmlands is easy to cause are overcome, the fungi and / or the bacteria are fully used for decomposition metabolism of residual quinclorac in the soil, the problems that the highly residual quinclorac in the soil seriously endanger food safety can be overcome, the micro-ecology of the soil is improved, the farming ability of the soil is faster to recover, and the method is low cost, and has broad application prospects.

In the present invention, by amplifying a strong promoter in S. adhesica, bioinformatics analysis and functional verification are carried out to obtain vitamin B-producing ingredients that can be widely used in Sinorhizobium meliloti, Pseudomonas denitrification and S. adhesica. 12 A strong promoter for gene expression, genetic manipulation and strain improvement in strains, the nucleotide sequence of which is SEQ ID NO:1. The present invention also relates to a plasmid vector containing the strong promoter, a method for constructing a genetically engineered bacterial strain using the promoter, a corresponding bacterial strain, and the application of host cells in promoting the expression of an objective gene.

The invention provides a microbial agent and a preparation method and application thereof. The microbial agent comprises the ingredients: Microbacterium sp. CICC 23615, Pseudomonas denitrificans CCTCC M91003, Deinococcus sp. CICC 10697, Bacillus sp. CICC 21937, Pichia pastoris ACCC 21021, and Aspergilus flavus CGMCC 3.2823. The microbial agent provided herein can remove ammonia and nitrogen in wastewater effectively in a biological treatment stage, up to about 97% in removal rate; in early startup stage of a system or in case of unstable operation, the microbial agent has excellent impact resistance. The microbial agent is free of pathogens, heavy metals and toxic and harmful chemicals, and solid products of the microbial agent are nontoxic and nonirritating.