8 results about "Pseudomonas denitrificans" patented technology

The application discloses an ecological purification and restoration agent for eutrophication landscape water body and a restoration method, and belongs to the technical field of water, waste water, sewage or sludge treatment. The ecological purification and restoration agent for eutrophication landscape water body comprises the following components in parts by weight: 2.4-5.5 parts of a composite microbial agent, 3.5-6.0 parts of a porous functional polymer material, 12-16 parts of bentonite, and 18-30 parts of clinoptilolite. The porous functional polymer material is adopted to reduce the content of nitrogen and phosphorus in a short time through chemical adsorption, and the composite microbial agent prepared according to a specific proportion of bacillus subtilis, bacillus amyloliquefaciens, pseudomonas denitrificans and nitrobacter winogradskyi is used to comprehensively restore the water body, so that the eutrophication of the water body is effectively relieved, and the cost is low and the purification is complete.

The invention discloses a whole-process denitrifying pseudomonas and application thereof in textile printing and dyeing sewage, and relates to the field of environmental microorganism water environment governance, the key points of the technical scheme are as follows: the whole-process denitrifying pseudomonas is pseudomonas stutzeri S8 and is preserved in the China Center for Type Culture Collection (CCTCC), the preservation date is June 23, 2025, and the preservation number is CCTCC NO: M 20251451. The Pseudomonas stutzeri S8 can simultaneously perform whole-course denitrification and decolorization under the toxicity stress of hexavalent chromium ions, the removal rate of nitrate and nitrite in chromium-containing actual textile printing and dyeing sewage can be higher than 95% after standing culture is performed for 48 hours, the synchronous dye removal rate is higher than 82%, the tolerant nitrate and nitrite concentration is higher than 500 mg / L, and the application range is wide. The strain has the advantages that the concentration of hexavalent chromium ions tolerated by the strain is higher than 50mg / L, in addition, the strain also has good decoloring and denitrification effects in a decoloring and denitrification culture medium stressed by other five single dyes, the nitrate removal rate is higher than 98%, and the dye removal rate is 70%-100%.

The invention relates to a high-yield vitamin B12 strain with improved robustness and application of the high-yield vitamin B12 strain, and belongs to the technical field of microbial fermentation and metabolic engineering. According to the invention, a pseudomonas denitrificans original production strain capable of synthesizing the vitamin B12 is evolved by a long-term adaptive laboratory taking the vitamin B12 and a structural analogue thiamine hydrochloride thereof as selection pressure. The evolved strain shows significantly enhanced growth ability and fermentation stability in a high stress environment, and the highest yield of vitamin B12 can reach 314 mg / L, which is 38.7% higher than that of an original strain, which is the highest level reported at present. Moreover, stable production can be realized on a 7 L fermentation tank, and good batch stability is realized. The invention also provides application of the strain in industrial production of the vitamin B12, and the strain has important significance in reducing the production cost of the vitamin B12 and improving the fermentation efficiency.

The invention discloses a multi-bacteria enzyme composition for water purification treatment and application, and relates to the technical field of water purification. The microbial agent is prepared from the following components in parts by weight: 15 to 30 parts of rhodopseudomonas palustris, 15 to 30 parts of bacillus subtilis, 25 to 35 parts of serratia marcescens, 25 to 35 parts of denitrifying pseudomonas, 25 to 35 parts of lactic acid coccus, 25 to 35 parts of bacillus amyloliquefaciens and 25 to 35 parts of rhodotorula benthica. The synergistic effect of the flora can inhibit the outbreak of harmful algae, promote the growth of beneficial algae, improve the flocculation performance of a water body and effectively improve the transparency. Meanwhile, the composition has a remarkable removal effect on key pollution indexes such as ammonia nitrogen, COD (Chemical Oxygen Demand), BOD (Biochemical Oxygen Demand), suspended solids and total phosphorus, so that the systematic repair of the water body is realized.

The application discloses a kind of preparation and application of reinforced sulfur autotrophic denitrification composite carrier filler, belong to wastewater treatment technical field.The composite carrier and embedding are included;The composite carrier is formed by polyvinyl alcohol and biochar, and the embedding is formed by riboflavin and denitrifying pseudomonas.The riboflavin, denitrifying pseudomonas and composite carrier are embedded together in the application, which can improve the mass transfer effect, effectively disperse riboflavin and bacterial body, avoid bacterial body loss, and help maintain the treatment capacity of denitrifying pseudomonas.

The present application provides a kind of fermentation method for improving the content of vitamin B12, by controlling total sugar content to be 1.0-7.0g / 100ml in the fermentation process, under the condition that 5-amino levulinic acid is added in the fermentation process of producing vitamin B12 in the fermentation medium by using Pseudomonas denitrificans, the metabolic pathway of vitamin B12 product synthesis is greatly strengthened.The fermentation method of the present application shortens the fermentation period and effectively improves the fermentation titer in the fermentation process of producing vitamin B12, and has great industrial application prospect.

The invention relates to a compound microbial agent for treating landfill leachate. The compound microbial agent is prepared from the following strains: paracoccus Wheatstone, rhodococcus ruber, pichia pastoris, bacillus subtilis, pseudomonas denitrificans, pseudoochrobactrum saccharum and rhodopseudomonas palustris. Wherein the paracoccus Wheatstone and the pseudoochrobactrum anthropi are preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation numbers of the paracoccus Wheatstone and the pseudoochrobactrum anthropi are respectively CGMCC No.17359 and CGMCC No.31632. Extracellular enzymes and intracellular enzymes secreted in the metabolism process of all strains in the compound microbial agent are cooperated, the compound microbial agent has good adaptability to various landfill leachate from different sources, pollutants such as COD, ammonia nitrogen and heavy metal in the landfill leachate can be efficiently degraded, and the overall impact resistance and degradation performance of a biochemical system are greatly improved.

The invention relates to the technical field of biological fermentation, in particular to a method for producing vitamin B12 by aerobic fermentation of Pseudomonas denitrificans, which comprises the following steps: breeding Pseudomonas denitrificans strains, and inoculating to an optimized slant culture medium for activation culture; preparing a seed culture medium, and inoculating the activated strain into the seed culture medium for culturing according to the inoculum size; preparing a fermentation culture medium, and modifying the fermentation culture medium; inoculating the seed solution into a fermentation culture medium according to the inoculum size, and carrying out aerobic fermentation culture; sodium citrate and betaine are added in the fermentation process, the dissolved oxygen concentration is controlled, and the pH value is monitored; after fermentation is finished, vitamin B12 is extracted and purified. According to the method for producing the vitamin B12 through aerobic fermentation of the pseudomonas denitrificans, the yield and quality of the vitamin B12 are remarkably improved by obtaining strains with high synthesis capacity, optimizing components and proportions of a slant culture medium, a seed culture medium and a fermentation culture medium and adopting an adsorbent preparation technology.