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Pseudomonas denitrificans is a Gram-negative aerobic organism bacterium that performs denitrification. It was first isolated from garden soil in Vienna, Austria. It synthesizes vitamin B₁₂. Based on 16S rRNA analysis, P. denitrificans has been placed in the P. pertucinogena group.
A fermentation method for increasing the content of vitamin b12
PendingCN122405779AAmino-Levulinic AcidVitamin B12
The present application provides a kind of fermentation method for improving the content of vitamin B12, by controlling total sugar content to be 1.0-7.0g / 100ml in the fermentation process, under the condition that 5-amino levulinic acid is added in the fermentation process of producing vitamin B12 in the fermentation medium by using Pseudomonas denitrificans, the metabolic pathway of vitamin B12 product synthesis is greatly strengthened.The fermentation method of the present application shortens the fermentation period and effectively improves the fermentation titer in the fermentation process of producing vitamin B12, and has great industrial application prospect.
Owner:NINGXIA KINGVIT PHARMA