A fermentation method for increasing the content of vitamin b12
By adding 5-aminolevulinic acid and controlling the total sugar content during the vitamin B12 fermentation process, the fermentation conditions were optimized, solving the problems of long fermentation cycle and low production level. This resulted in a shorter fermentation cycle and improved potency, making it suitable for industrial production.
CN122405779APending Publication Date: 2026-07-17NINGXIA KINGVIT PHARMA
View PDF 0 Cites 0 Cited by
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-01-13
- Publication Date
- 2026-07-17
AI Technical Summary
Technical Problem
Existing vitamin B12 fermentation processes suffer from long fermentation cycles and low production levels, and fail to improve the potency of fermentation products through effective regulation of metabolic pathways.
Method used
During fermentation, a specific precursor, 5-aminolevulinic acid, is added, and the total sugar content is controlled at 1.0-7.0 g/100 ml. Fermentation conditions such as temperature, pressure, air flow rate, and stirring speed are optimized to shorten the fermentation cycle and enhance the metabolic pathway of vitamin B12.
Benefits of technology
It effectively shortened the fermentation cycle by 5.95%, increased the fermentation potency by 9.74%, met the needs of industrial production, and reduced production costs.
✦ Generated by Eureka AI based on patent content.
Smart Images
Figure CN122405779A_ABST
Abstract
The present application provides a kind of fermentation method for improving the content of vitamin B12, by controlling total sugar content to be 1.0-7.0g / 100ml in the fermentation process, under the condition that 5-amino levulinic acid is added in the fermentation process of producing vitamin B12 in the fermentation medium by using Pseudomonas denitrificans, the metabolic pathway of vitamin B12 product synthesis is greatly strengthened.The fermentation method of the present application shortens the fermentation period and effectively improves the fermentation titer in the fermentation process of producing vitamin B12, and has great industrial application prospect.
Need to check novelty before this filing date? Find Prior Art