A method for separating wheat prolamin by capillary electrophoresis
A technology of wheat prolamin and capillary electrophoresis, applied in the direction of material analysis, analysis materials, instruments, etc. by electromagnetic means, can solve the problems of laborious resolution and time-consuming
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[0064] Embodiment 1, capillary dynamic coating separation wheat prolamin
[0065] 1. Materials
[0066] Gliadin standard products were purchased from Tokyo Kasei Kogyo Co., Ltd.
[0067] The five wheat varieties are Zhoumai 22, Jimai 22, Liangxing 99, Aikang 58 and Laizhou 953.
[0068] Preparation of gliadin standard substance: Accurately weigh 50 mg of gliadin standard substance, dissolve it in 2 mL, 75% (volume) ethanol aqueous solution, the final concentration is 25 mg / mL.
[0069] Preparation of wheat seed samples: Wheat seeds were crushed directly, baked at 37°C for one week, passed through a 100-mesh sieve, and then extracted according to the following method 1 and method 2:
[0070] Method 1: Accurately weigh 100mg of wheat seed samples, add 1mL of 0.1M sodium chloride aqueous solution respectively, shake and extract at room temperature for 30 minutes, centrifuge (12000r / min) for 10 minutes, discard the supernatant, add 1mL of 0.1M sodium chloride aqueous solution ag...
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