50 results about "Gliadin IgG" patented technology

A food composition comprises gluten, a gliadin or glutenin and food stuff such as an additive for chewing gum, a batter for frying, a dough, a seafood paste and a livestock paste. A food quality such as taste is improved in the composition.

The invention relates to a resveratrol oral administration drug delivery system prepared through the nanometer technology. The drug delivery system is made of the resveratrol and bearer material of wheat gliadin. By utilizing the characteristic that the nanometer granule is highly dispersed and that of biological adhesion of the bearer material, the contact area and contact time of the resveratrol and absorption region of the intestinal canal is increased, which can promote absorption of the small intestine. In addition, the resveratrol oral administration nanometer drug delivery system is distributed over the suitable auxiliary-material skeletal material to improve the dissolve velocity and degree of the resveratrol.

The present invention relates to compositions for ameliorating the symptoms of celiac disease or gluten sensitive enteropathy comprising egg yolk antibodies against gluten, including gliadin, high molecular glutenin, low molecular glutenin and mixtures of the three peptides. The antibodies may be produced by immunizing laying hens with immunogenic preparations of gluten and harvesting the eggs and egg yolks.

The invention belongs to the technical field of processing of grain active protein and in particular relates to wheat alcohol-soluble protein and glutelin as well as a preparation method and an application thereof. The preparation method of the wheat alcohol-soluble protein and the glutelin takes wheat meal as the raw material and comprises the following steps: extracting with an alkaline alcohol solution, settling and separating, concentrating, separating and purifying, and drying to prepare the wheat alcohol-soluble protein and the glutelin. The wheat alcohol-soluble protein and the glutelin prepared by the method are high in activity, excellent in viscoelasticity and extensibility and especially applied to production of rice and flour products such as steamed bun, noodles, bread, rice noodles and soft sweets or gelled foods prepared by the rice and flour products.

A composition for lowering the glycemic index (GI value) of food or feed, comprising bean protein and degraded galactomannan of leguminous plant guar; a low glycemic index food, comprising Polygalactosylmannose and gliadin and glutenin; a low glycemic index food comprising polygalactose and/or polygalactose derivatives and gliadin and glutenin in specific proportions; a low A glycemic index food comprising polygalactosylmannose and amylose and amylopectin in specified proportions; a low glycemic index food comprising specified polygalactose and/or polygalactose derivatives and amylose in specified proportions Starch and amylopectin.

A preparation method of wheat protein fibers. The invention belongs to the technical field of wheat protein resource utilization. The method includes the steps of: performing acetylation to purified gliadin, and mixing the gliadin with glutenin according to ratio of 4-2:1-2; dissolving the mixture with a reducing agent and an oxidizing agent, and treating the solution at certain ultrasonic power and microwave power to prepare a spinning solution; perform spin-drawing, and treating the spinning product with a citric acid solution in certain concentration instead of glutaraldehyde and formaldehyde to prepare the high-strength wheat protein fiber. The wheat protein fiber has a soft and smooth hand feel, wherein dry state breaking strength thereof is 0.9-1.2 cN/dtex and dry state breaking elongation thereof reaches 50-70%. The wheat protein fiber is 10-13% in moisture regain at 20 DEG C under the relative humidity of 65%.

Provided is a mAb-based method for the detection of T cell stimulatory epitopes known to be involved in CD. The method has many advantages compared to the existing methods for the detection of gluten since it is the first method that can; (i) detect T cell stimulatory epitopes of gluten; (ii) detect the epitopes separately, (iii) detect T cell stimulatory epitopes present on gliadin and glutenin homologues present in other cereals also known to be involved in CD; and (iv) detect T cell stimulatory epitopes on both intact proteins and small protein fragments. The new method is a valuable tool in the screening of basic ingredients, semi manufactured ingredients and food products that are intended to be used in the gluten free diet of CD patients. Moreover the new method can also be used for the screening of cereals and different wheat varieties for the level of toxicity for CD patients. Thereby the method can help in the selection of cereals and wheat varieties with low toxicity which might form the basis for future breeding programs. In the future these cereals will be used for the production of safe food for CD patients.

The invention discloses a nanoporous magnesium silicate microsphere/PBS (poly(butylene succinate)) composite scaffold, a composite scaffold coated with protein, preparation methods and an application. A preparation method of a genipin crosslinking protein coating supported drug-nanoporous magnesium silicate microsphere/PBS composite scaffold comprises the following steps: PBS is mixed with an organic solvent, the mixture is then mixed with nanoporous magnesium silicate microspheres and a pore-foaming agent, the mixture is subjected to pressing forming, the organic solvent and the pore-foaming agent are removed, and the composite scaffold is obtained after drying; the composite scaffold is soaked in a resveratrol buffer solution, and the resveratrol supported composite scaffold is obtained after drying; a gliadin solution is added to the resveratrol supported composite scaffold, the gliadin protein coating supported composite scaffold is obtained after drying and then mixed with a genipin solution for a crosslinking reaction, and a product is obtained. The composite scaffolds have higher in-vitro degradation performance and bioactivity, good cell compatibility, high drug adsorption capacity and good drug slow release effect, and the effective acting time of drugs can be prolonged.

The present invention relates to the specific silencing of the α (alpha), β (beta), γ (gamma) and ω (omega)-gliadins of hard wheat for flour by RNA interference (RNAi) through employment of a polynucleotide which is transcribed into an hpRNA (hairpin RNA). Furthermore the present invention additionally relates to a vector, cell, plant or seed comprising the polynucleotide, the expression whereof is specifically directed in particular tissues of wheat seeds through gene expression-regulating sequences such as, for example, the promoter of a gene of γ-gliadins or the promoter of the gene encoding for a D-hordein.

Disclosed is a bio-based and environmentally friendly elastomer product from renewable agricultural substrates. Specifically, glutamine and arginine residues of gliadin are used as synthons to produce novel elastomeric product from the reaction of the oxirane groups of epoxidized vegetable oils under neat reaction conditions with the primary amide functionalities of glutamine and arginine to give the corresponding amidohyroxy gliadinyl triglycerides.

The invention discloses high-permeability environment-friendly antibacterial bath foam which is prepared from the following raw materials in parts by weight: 0.01-0.02 part of garlicin, 2-3 parts of cocamidopropyl betaine, 0.6-1 part of hydrogenated castor oil, 0.2-0.3 part of glycerol monooleate, 0.5-0.7 part of phytosterin, 0.8-2 parts of canola oil, 0.1-0.2 part of nutrient alkali liquor, 0.7-1 part of oxidized alkylamine polyoxyethylene ether, 130-150 parts of water, 0.2-0.3 part of soda and 0.02-0.03 part of gliadin. According to the high-permeability environment-friendly antibacterial bath foam, the phytosterin which is added into the raw materials has very high permeability on skin, is capable of keeping the moisture on the surface of the skin, promoting metabolism of the skin, inhibiting inflammation of the skin as well as preventing sunburn and skin aging, and has the effects of regenerating and nourishing hair; the added garlicin can obviously increase the antibacterial effect of a finished product; and the added nutrient alkali liquor can improve the vitality of the skin. The bath foam disclosed by the invention is fragrant, nontoxic, environment-friendly and good in safety.

The invention prevents or treats a tumor, inhibiting tumor growth and malignant progression by providing an antitumor agent containing a treated SOD as the active ingredient, especially a melon SOD coated with gliadin. The invention also applies to a method of preventing or treating the tumor.

The present invention relates to a new medicine delivery system carrier material, namely liver target nanometer combination that is made from wheat gliadin and silibinin. The preparation method of the nanometer combination is also provided. Coprecipitation of medicine and material in an identical system is achieved in a desolvation way; the nanometer combination colloidal solution that is made in the method can be produced into freeze-dried power injection after being mixed with the freeze-dried supporting agent acceptable in pharmacy. The nanometer combination can improve blood concentration of the medicine in liver region so that effect of the hepatitis cure medicine can be better exerted.

The invention discloses a method for separating wheat gliadin through capillary electrophoresis. The method comprises the steps as follows: CPVP (cationic polyvinylpyrrolidone) is used as a material, and a coating is prepared on the wall of a capillary; a wheat sample is separated through capillary electrophoresis, and thus, wheat gliadin is separated; the CPVP is cationized polyvinylpyrrolidone with the viscosity average molecular weight of 20,000-25,000; the coating is prepared in the following step: the quartz capillary is washed with an aqueous solution of the CPVP and an operation buffer solution A sequentially. The CPVP is used as the coating material, dynamic modification is performed on a capillary column, the extraction separation condition suitable for wheat gliadin is screened out through optimization, a technological method for stably separating wheat gliadin is established, different varieties of wheat gliadin can be effectively distinguished, and the method becomes a wheat variety fingerprint separation technology.

The invention discloses a wheat prolamine monoclonal antibody hybridoma cell strain and its application, and belongs to the technical field of food safety immunodetection. The wheat prolamine monoclonal antibody hybridoma cell strain CS6-1 has been preserved in the China General Microbiological Culture Collection Center; the preservation number is CGMCC NO.14687. The invention acquires the hybridoma cell strain capable of secreting wheat prolamine monoclonal antibody; an antibody 4F2 secreted by cell strain CS6-1 is a coating antibody; the antibody 4F2 is marked with HRP and then formed to beenzyme labelled antibody (4F2-HRP); the wheat prolamine is used as a standard product, and the wheat prolamine dual-antibody enzyme-linked immunosorbent assay method is built up; the wheat prolamine monoclonal antibody hybridoma cell strain has actual application value in detecting food allergens.

The invention relates to a wheat gluten protein separation and purification processing technology, and discloses a method for separating wheat alcohol-soluble protein by utilizing a sucrose density gradient centrifugation method. Firstly, sucrose is dissolved in water to prepare solutions with different mass percent concentrations, then a to-be-separated alcohol-soluble protein solution soluble in70% ethanol is injected into the bottom of a centrifugal tube; the 10-70% sucrose density gradient centrifugate is prepared through the layer spreading method; centrifugation is conducted on 5000-20000 g of centrifugate for 10-60 min at the temperature below 4 DEG C, after alcohol-soluble protein settles and is balanced, a sucrose solution with the corresponding concentration is obtained, the protein content in the layer, where the protein is located, of the sucrose solution is measured under the wavelength of 280 nm by means of a spectrophotometer, and the alcohol-soluble protein can be separated into light alcohol-soluble protein, intermediate alcohol-soluble protein and heavy alcohol-soluble protein according to different sucrose densities. The method is suitable for separating and purifying the alcohol-soluble protein, results are accurate, the repeatability is good, operation is easy and feasible, and the quantity of required samples is small.