The Group Breeding Method of Guangdong Twig Bean

A technology of twig bean and seedling hardening, which is applied in the fields of agricultural biology and applied biology, can solve the problems of few twig seeds and restrict production development, and achieve a short seedling cycle, stable and reliable results, and low requirements for experimental facilities Effect

Active Publication Date: 2017-01-11
广州市鲸力生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is an urgent need to research and develop the tissue culture technology of Tachibana japonica, and realize the large-scale propagation of Tachibana japonica seedlings through tissue culture technology, so as to solve the problem that there are few seeds of Tachibana japonica in Guangdong, which restricts the production and development

Method used

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  • The Group Breeding Method of Guangdong Twig Bean
  • The Group Breeding Method of Guangdong Twig Bean
  • The Group Breeding Method of Guangdong Twig Bean

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046](1) Seed disinfection and germination: The seeds of Millettia fordii Dunn (Millettia fordii Dunn) in Guangdong were purchased from Guangzhou Dayou Yuxuan Agricultural Development Co., Ltd; On the clean workbench; pour 75% alcohol into the disinfection bottle, shake it left and right, disinfect it for 8 minutes, and rinse it twice with sterile water; then pour 1g / L mercuric chloride for disinfection for 4 minutes, and finally rinse it with sterile water for 4 minutes. times; placed in sterile moist gauze, covered with gauze seeds. The pollution-free rate of seeds after disinfection can reach 70.6%.

[0047] (2) Select explant: 15 days after seed inoculation, begin to germinate; In 30 days, germinate grows to 3cm high, select the healthy and strong germ bar that does not pollute, after cutting off radicle and cotyledon, get hypocotyl as explant. The total germination rate reached 85.0%.

[0048] (3) Proliferation of adventitious buds: the explants obtained in step (2) we...

Embodiment 2

[0052] (1) Seed disinfection and germination: The seeds of Millettia fordii Dunn (Millettia fordii Dunn) in Guangdong were purchased from Guangzhou Dayou Yuxuan Agricultural Development Co., Ltd; On the clean workbench; pour 75% alcohol into the disinfection bottle, shake it left and right, disinfect it for 12 minutes, and rinse it twice with sterile water; then pour 1g / L mercuric chloride for disinfection for 6 minutes, and finally rinse it with sterile water for 4 minutes times; placed in sterile moist gauze, covered with gauze seeds. The pollution-free rate of seeds after disinfection can reach 73%.

[0053] (2) Select explant: 15 days after seed inoculation, begin to germinate; In 30 days, germinate grows to 3cm high, select the healthy and strong germ bar that does not pollute, after cutting off radicle and cotyledon, get hypocotyl as explant. The total germination rate reached 85.2%.

[0054] (3) Proliferation of adventitious buds: the explants obtained in step (2) wer...

Embodiment 3

[0058] (1) Seed disinfection and germination: The seeds of Millettia fordii Dunn (Millettia fordii Dunn) in Guangdong were purchased from Guangzhou Dayou Yuxuan Agricultural Development Co., Ltd; On the clean workbench; pour 75% alcohol into the disinfection bottle, shake it left and right, disinfect it for 15 minutes, and rinse it twice with sterile water; then pour 1g / L mercuric chloride for disinfection for 8 minutes, and finally rinse it with sterile water for 4 minutes. times; placed in sterile moist gauze, covered with gauze seeds. The pollution-free rate of seeds after disinfection can reach 90%.

[0059] (2) Select explant: 15 days after seed inoculation, begin to germinate; In 30 days, germinate grows to 3cm high, select the healthy and strong germ bar that does not pollute, after cutting off radicle and cotyledon, get hypocotyl as explant. The total germination rate is 86%.

[0060] (3) Proliferation of adventitious buds: the explants obtained in step (2) were inoc...

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Abstract

The invention discloses a tissue culture seedling-raising method of millettia fordii dunn. The method comprises the following steps: disinfecting and sprouting seeds, selecting germinal sterile embryo axes as explants, carrying out adventitious bud proliferation, carrying out rooting culture, hardening seedlings and transplanting the seedlings to obtain millettia fordii dunn seedlings, wherein a multiplicative culture medium comprises an MS culture medium, 0.3-0.5mg / L of 6-BA, 0.06-0.1mg / L of TDZ, 0.15-0.3mg / L of NAA, 30g / L of cane sugar and 5.8g / L of carrageenan; the pH value is 5.5-5.8. The millettia fordii dunn seedlings cultured by the method has the characteristics of good hereditary stability, high reproduction speed, short seedling raising period, low cost and simplicity in operation; the experimental facility requirements are low; the operation process is simple; the millettia fordii dunn seedlings are bred in scales; the result is stable and reliable.

Description

technical field [0001] The invention belongs to the fields of applied biology and agricultural biotechnology, and in particular relates to a method for group-cultivating seedlings of twigs from Guangdong. Background technique [0002] Millettia fordii Dunn (Millettia fordii Dunn) is produced in Guangdong and Guangxi, and grows in the valley forest at an altitude of about 500 meters. Vines; pinnately compound leaves; racemes usually axillary; pods linear; seeds brown, ovate, about 7 mm long, about 6 mm wide, smooth; flowering period from September to October, fruiting period in November. [0003] Folks believe that Tachibana japonica has the effects of nourishing the lungs and relieving cough, strengthening the spleen and tonifying deficiency, strengthening yang and tonifying the kidney. Therefore, in the Wuzhou area of ​​Guangxi and the Maofeng Mountain area of ​​Guangzhou, it is popular to use the roots of the twig and pork bones to make soup. Due to artificial and disord...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00A01G1/00
Inventor 曾雷谢鉴能温依娇
Owner 广州市鲸力生物科技有限公司
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