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A high-efficiency assisted selection method for molecular markers in the restorer line of japonica rice

A technology assisted by molecular markers and assisted selection, which is applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problems of difficult measurement technology, cumbersome procedures, and low efficiency, and achieve good heterosis and expand Background, the effect of improving selection efficiency

Active Publication Date: 2022-06-21
辽宁省水稻研究所
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AI Technical Summary

Problems solved by technology

Molecular marker-assisted selection overcomes the difficulties of phenotypic identification of traits: the phenotype identification of some traits is quite difficult, and the restorer line cannot be identified from the phenotype. Low; the genotype after matching identification is quite different from the expected one

Method used

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  • A high-efficiency assisted selection method for molecular markers in the restorer line of japonica rice
  • A high-efficiency assisted selection method for molecular markers in the restorer line of japonica rice
  • A high-efficiency assisted selection method for molecular markers in the restorer line of japonica rice

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Embodiment 1

[0019] A high-efficiency assisted selection method for molecular markers of japonica rice classic dyed and spoiled restorer lines, comprising the following operation steps:

[0020] (1) Using primers RM5629 and RM10353 to label the first and ten chromosome restorer genes SSR of the four rice parents, late round 422, R498, R527, and C418, respectively, and then amplified and electrophoresed to obtain the restorer genes of the four rice parents. The genotype of the restorer line of R498 is Rf1Rf1Rf10Rf10, the genotype of the restorer line of R527 is Rf1Rf1Rf10Rf10, the genotype of the restorer line of C418 is Rf10Rf10, and the genotype of the restorer line of late round 422 is Rf1Rf1Rf10Rf10;

[0021] (2) Hybrid between the four rice parents: late round 422 / R498, late round 422 / R527, C418 / R498, C418 / R527, and then obtain F 2 The results of gene marker-assisted selection in the restorer line of the generation population, in F 2 The restorer lanes for molecular marker-assisted se...

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Abstract

The invention discloses a method for highly efficient assisted selection of molecular markers for japonica rice restorer lines, comprising the following steps: (1) using primers to mark the first and tenth chromosome restorer genes SSR of four rice parents respectively, and then performing amplification and electrophoresis , to obtain the restorer line genotypes of the four rice parents; (2) cross the four rice parents, and then obtain F 2 The molecular marker-assisted selection results of gene population restorer line, in F 2 The restorer line lanes of molecular marker-assisted selection in generation segregation population; (3) for F 4 generation by molecular marker-assisted selection. A large number of restorer lines and a wide range of restorer types can be selected in a short period of time by molecular marker-assisted selection, which saves manpower, material resources and financial resources.

Description

technical field [0001] The invention belongs to the technical field of molecular marker application, and in particular relates to a high-efficiency assisted selection method for molecular markers of japonica rice classic dyeing and spoilage restorer lines. Background technique [0002] In recent years, molecular breeding has developed rapidly, and favorable genes from distant or wild rice have been successfully introduced into excellent rice seed resources in many studies. Molecular breeding for disease resistance is a successful example of molecular marker-assisted selection. Obtained a new line with improved horizontal and vertical resistance to leaf blight; cultivated a number of bacterial blight-resistant restorer lines with Xa-21 gene; combined with the genetic map of the rice blast resistance gene Pi25, newly added and this The closely linked molecular markers RM3330 and A7, combined with the results of molecular marker-assisted selection, and the use of markers linke...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895
CPCC12Q1/6895C12Q2600/13
Inventor 王先俱张城庞秀姜伟陈亚君丁芬
Owner 辽宁省水稻研究所
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