Molecular marker assisted efficient selection method of typical/stained abortion type restorer lines of japonica rice

A molecular marker-assisted and assisted selection technology, which is applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problems of cumbersome procedures, low efficiency, and difficult measurement technology, so as to expand the genetic background and improve selection. Efficiency, the effect of good heterosis

Active Publication Date: 2019-03-19
辽宁省水稻研究所
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Problems solved by technology

Molecular marker-assisted selection overcomes the difficulties of phenotypic identification of traits: the phenotype identification of some traits is quite dif

Method used

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  • Molecular marker assisted efficient selection method of typical/stained abortion type restorer lines of japonica rice
  • Molecular marker assisted efficient selection method of typical/stained abortion type restorer lines of japonica rice
  • Molecular marker assisted efficient selection method of typical/stained abortion type restorer lines of japonica rice

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Embodiment 1

[0019] A high-efficiency assisted selection method for molecular markers of japonica rice strain restorer lines, comprising the following steps:

[0020] (1) Use primer RM5629 and primer RM10353 to mark the first and tenth chromosome restorer genes SSR of four rice parents, late round 422, R498, R527, and C418, and then amplify and perform electrophoresis to obtain restorer genes of four rice parents The restorer genotype of R498 is Rf1Rf1Rf10Rf10, the restorer genotype of R527 is Rf1Rf1Rf10Rf10, the restorer genotype of C418 is Rf10Rf10, and the restorer genotype of Wanlun 422 is Rf1Rf1Rf10Rf10;

[0021] (2) Crossing Wanlun 422 / R498, Wanlun 422 / R527, C418 / R498, C418 / R527 between four rice parents, and then obtaining F 2 The molecular marker-assisted selection results of gene population restorer line, in F 2 The restorer line lane of molecular marker-assisted selection in generation segregation population, genotype Rf1Rf1Rf10Rf10, so in F 2 Two restorer lines with homozygous...

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Abstract

The invention discloses a molecular marker assisted efficient selection method of typical/stained abortion type restorer lines of japonica rice. The method herein includes the steps of (1) using primers to mark first and tenth chromosome restoring genes SSR of four rice parents respectively, performing amplifying before electrophoresis to obtain restorer line genotypes of the four rice parents; (2) hybridizing the four rice parents to obtain F2-generation population restorer line gene molecular-marker-assisted selection results, and subjecting F2-genernation segregation population to molecular-marker-assisted selection to obtain a restorer line lane; (3) subjecting F4 generation to molecular-marker-assisted selection. More restore lines under wide restorer type range can be attained withina short time by molecular-marker-assisted selection; manpower, material and financial resources can be saved.

Description

technical field [0001] The invention belongs to the technical field of molecular marker application, and in particular relates to a highly efficient and assisted selection method for molecular markers of japonica rice strains. Background technique [0002] In recent years, molecular breeding has developed rapidly, and many studies have successfully introduced favorable genes from distant species or wild rice into excellent rice seed resources. Molecular breeding for disease resistance is a successful example of molecular marker-assisted selection. Obtained new lines with improved horizontal and vertical resistance to leaf blight; bred a batch of restorer lines with Xa-21 gene for resistance to bacterial blight; combined with the genetic map of rice blast resistance gene Pi25, the newly added The closely linked molecular markers RM3330 and A7, combined with the results of molecular marker-assisted selection, and the use of markers linked on both sides of the target gene for ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895
CPCC12Q1/6895C12Q2600/13
Inventor 王先俱张城庞秀姜伟陈亚君丁芬
Owner 辽宁省水稻研究所
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