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Method for cultivating polyploid poplar and application of thereof

A technology of polyploidy and poplar, applied in the fields of application, botany equipment and methods, cultivation, etc., can solve the problems of high technical level requirements for aseptic operation, heavy test workload, complicated operation steps, etc., and improve breeding Efficiency, less workload, shortening the effect of breeding cycle

Active Publication Date: 2020-12-11
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This tetraploid induction method is highly dependent on sterile tissue culture operations. It is necessary to establish an efficient leaf adventitious bud regeneration system. The experimental workload is large, the cycle is long, the operation steps are complicated, and the technical level of aseptic operation is high.

Method used

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  • Method for cultivating polyploid poplar and application of thereof
  • Method for cultivating polyploid poplar and application of thereof
  • Method for cultivating polyploid poplar and application of thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] The poplar test material selection research group used in this embodiment collected the hard branch cuttings of the diploid poplar species 'Huilin 88' from Tongliao Academy of Forestry, Inner Mongolia, with a thickness of 2.2 cm and a length of 12 cm.

[0041] 1. Cuttings

[0042] In the greenhouse of Beijing Forestry University, the cuttings of 'Huilin 88' were placed in a flowerpot of 25cm (inner diameter) × 25cm (height), and the cutting substrate sterilized by high temperature and high pressure was filled in the flowerpot, and the substrate was peat soil: Perlite: river sand = 2:1:1, water the cuttings thoroughly, cover with plastic film and cultivate at 15-25°C and 50-80% relative humidity.

[0043] 2. Double processing

[0044] About 3 days after cutting, callus formation can be observed in the cambium area of ​​the cutting surface ( figure 1 ), the callus developed further after cutting 8 days. At this point, completely cover the cut surface of the cuttings with...

Embodiment 2

[0052] The test material of this embodiment is the tetraploid hard branch cuttings of Populus papillodis that were induced by the research group in the early stage, with a thickness of 2.6 cm and a length of 12 cm.

[0053] 1. Cuttings

[0054] In the greenhouse of Beijing Forestry University, the cuttings of the tetraploid Populus pachyrhiza were cut in a flowerpot of 25cm (inside diameter) × 25cm (height), and the cutting substrate sterilized by high temperature and high pressure was filled in the flowerpot, and the substrate was peat soil : perlite: river sand = 2:1:1, water the cuttings thoroughly, and cover with plastic film to cultivate at 15-25°C and 50-80% relative humidity.

[0055] 2. Double processing

[0056] About 5 days after cutting, callus formation can be observed in the cambium area of ​​the cutting surface, and the callus further develops after 10 days of cutting. At this point, completely cover the cut surface of the cuttings with absorbent cotton, add dr...

Embodiment 3

[0064] The poplar test material selection research group used in this embodiment collected the hard branch cuttings of the diploid poplar species 'Huilin 88' from Tongliao Academy of Forestry, Inner Mongolia, with a thickness of 2.2 cm and a length of 12 cm.

[0065] 1. Hydroponics

[0066] In the greenhouse of Beijing Forestry University, the hard branch cuttings of 'Huilin 88' were hydroponically cultured in a 10cm (upper inner diameter) × 20cm (height) plastic water cup filled with tap water and placed at 15-25°C and 50-80°C. % relative humidity and change the water every 2 days.

[0067] 2. Double processing

[0068] After hydroponics for about 6 days, callus formation can be observed in the cambium area of ​​the cutting surface, and the callus develops further after hydroculture for 10 days. At this point, completely cover the cut surface of the cuttings with absorbent cotton, add dropwise 0.05% colchicine solution to avoid light for 24 hours, remove the absorbent cotto...

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Abstract

The invention relates to a method for cultivating polyploid poplar and application thereof. The method comprises the steps that poplar branches are inserted into a culture medium and cultured for 3-10days at the temperature of 15-25 DEG C and the relative humidity of 50%-80%, and calluses are formed by notches in the poplar branches; and the calluses are treated by using a polyploid mutagenic agent until the calluses are fully mutated, treatment is ended, and culturing is continuously carried out. It is found that the notches in the poplar cutting branches can form the calluses after culturing is carried out under proper temperature and humidity conditions, polyploid poplar culture can be achieved by further applying a polyploid mutagenic agent for treatment, and the induction rate reaches about 42.9%. The method solves the technical problems of a conventional populus somatic chromosome doubling process or depending on embryology observation or depending on tissue culture and the like, has the advantages of easiness in operation, low cost, high induction efficiency, high preservation rate and the like, can ensure that induced polyploid seedlings grow up in the same year, and is anideal way for doubling populus somatic chromosomes.

Description

technical field [0001] The invention relates to the technical field of plant breeding, in particular to a method for cultivating polyploid poplars and an application thereof. Background technique [0002] The importance of polyploidy breeding for poplar genetic improvement has been widely recognized (Kang XY. Polyploid induction techniques and breeding strategies in poplar. In: Mason AS(ed) Polyploidy and hybridization for crop improvement. CRC Press, Boca Raton, 2016, pp 76–96), polyploid poplar species have exerted great value in existing forestry production. Somatic chromosome doubling is one of the important ways to induce polyploidy in poplar. This pathway is mainly to use chromosome doubling mutagen to treat somatic cells with meristematic ability to inhibit the separation of sister chromatids in metaphase of mitosis, thereby forming doubled cells and obtaining polyploidy. Somatic cell chromosome doubling is a very suitable autopolyploid induction method that can hig...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/08A01G2/10A01G24/10A01G24/12A01G24/15A01G24/28A01G31/00
CPCA01G31/00A01H1/08A01G2/10A01G24/10A01G24/12A01G24/15A01G24/28
Inventor 王君钟宇航李代丽
Owner BEIJING FORESTRY UNIVERSITY
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