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A method for large-scale breeding of virus-free lily balls

A lily seed ball, virus-free technology, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve problems such as affecting the yield and quality of lily, technical difficulty in operation, and difficulty in meeting large-scale production, etc. Easy to implement, simple process, unique effect of measures

Active Publication Date: 2021-07-20
LIANYUNGANG ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] With the expansion of lily production and bulb trade in the world, the production area of ​​lily and the demand for bulbs, however, during the growth of lily, the infection of external diseases and insect pests, and viral diseases began to be popular in various lily planting areas in my country, which not only seriously affected lily. The output and quality of lily bulbs have become the bottleneck of the industrialization of lily bulbs in my country.
At present, the research on the key technology of lily bulbs is mainly focused on the research on the comprehensive detoxification technology based on stripping the shoot tip. Although these methods can effectively remove the lily virus, they are technically difficult to operate and difficult to meet the scale. production requirements

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] A method for large-scale breeding of virus-free lily balls, the steps of the method are as follows:

[0038] a, ovary selection mark: look for the lily plant that grows normally in the field until the bud length is 9cm and the bud is uncolored and uncracked, choose the lily plant that grows thickly, and has no virus disease and other pests in the appearance of the character to pick the buds, and place them in the In the ice box with built-in ice cubes, after the flower buds are brought back, take out the complete ovary and mark each ovary;

[0039] b. Virus detection of ovary: cut the marked ovary from the bottom to the ovary with a length of 6 mm, and use multiple RT-PCR method to detect lily undocumented virus, cucumber mosaic disease and lily mottled virus. All virus ovaries were killed at high temperature and discarded. The test results showed that the cut ovaries without viruses were placed in a refrigerator at a temperature of 4°C for 7-8 days;

[0040] c. Ovary ...

Embodiment 2

[0044] A method for large-scale breeding of virus-free lily balls, the steps of the method are as follows:

[0045] a, ovary selection mark: look for the lily plant that grows normally in the field until the bud length is 7cm and the bud is uncolored and uncracked, choose the lily plant that grows strong, and the appearance of the character shows no virus disease and other pests and diseases, and picks the buds, and places them in the In the ice box with built-in ice cubes, after the flower buds are brought back, take out the complete ovary and mark each ovary;

[0046] b. Virus detection of ovary: cut the marked ovary from the bottom to the ovary with a length of 4mm, and use multiple RT-PCR method to detect lily undocumented virus, cucumber mosaic disease and lily mottled virus, showing that there are All virus ovaries were killed at high temperature and discarded. The test results showed that the cut ovaries without viruses were placed in a refrigerator at 0°C for 9-10 days...

Embodiment 3

[0051] A method for large-scale breeding of virus-free lily balls, the steps of the method are as follows:

[0052] a, ovary selection mark: look for the lily plant that grows normally in the field until the bud length is 10cm and the bud is uncolored and uncracked, choose the lily plant that grows strong, and the appearance of the character shows no virus disease and other pests and diseases. Pick the buds and place them in the In the ice box with built-in ice cubes, after the flower buds are brought back, take out the complete ovary and mark each ovary;

[0053] b. Virus detection of ovary: cut the marked ovary from the bottom to the ovary with a length of 7 mm, and use multiple RT-PCR method to detect lily undocumented virus, cucumber mosaic disease and lily mottled virus, showing that there are All virus ovaries were killed at high temperature and discarded. The test results showed that the cut ovaries without viruses were placed in a refrigerator with a temperature of 4°C...

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Abstract

The invention discloses a method for large-scale breeding of virus-free lily balls. The steps of the method are as follows: a. Ovary selection and marking: Lily plants are selected in the field, and the ovary is taken out and marked; b. Virus detection of the ovary: Cut out the ovary with a length of 4-7mm and use the multiple RT-PCR method for detection, and put the non-toxic ovary into the refrigerator for temporary storage; c, ovary pretreatment: rinse with running water, disinfect and sterilize, and then rinse with sterile water , the ovary is vertically cut into two parts and placed in the pretreatment medium for cultivation; d, ovary induced callus culture: the pre-cultured ovary is cut into 2mm cut pieces and placed in the induction medium for cultivation; e , Induction into balls: Transfer the callus to the medium for inducing buds, culture in the dark for 1-2 months and then subculture and multiply once. After 3-4 months, you can get lilies with a diameter of 0.3-2cm The small bulbs are the non-toxic bulbs of lilies. The process of the method of the invention is simple to operate and easy to be implemented on a large scale.

Description

technical field [0001] The invention relates to the technical field of seedlings, in particular to the technical field of cultivating lily bulbs, in particular to a method for large-scale breeding of virus-free lily bulbs by adopting lily ovary wall tissue culture. Background technique [0002] With the expansion of lily production and bulb trade in the world, the production area of ​​lily and the demand for bulbs, however, during the growth of lily, the infection of external diseases and insect pests, and viral diseases began to be popular in various lily planting areas in my country, which not only seriously affected lily. The output and quality of lily bulbs have become the bottleneck of the industrialization of lily bulbs in my country. At present, the research on the key technology of lily bulbs is mainly focused on the research on the comprehensive detoxification technology based on stripping the shoot tip. Although these methods can effectively remove the lily virus, t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 孙明伟赵统利邵小斌朱朋波汤雪燕王江英明军
Owner LIANYUNGANG ACAD OF AGRI SCI
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