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Method for preserving and separating toadstool strain through sporocarp dried in shade

A technology of morel mushrooms and shade-dried seeds, which is applied in the field of microbiology, can solve the problems of season limitation of the preservation method of morel mushroom species, easy separation of pollution, complicated operation, etc., and achieves the effects of low cost, low mutation probability, and easy operation

Active Publication Date: 2015-06-10
重庆市潼南区中药研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The technical problem to be solved by the present invention is that the existing hickory chick strain preservation method is limited by the season, and the operation is complicated, the cost is high, and the separation is easy to pollute

Method used

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  • Method for preserving and separating toadstool strain through sporocarp dried in shade
  • Method for preserving and separating toadstool strain through sporocarp dried in shade
  • Method for preserving and separating toadstool strain through sporocarp dried in shade

Examples

Experimental program
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Effect test

Embodiment 1

[0026] The dry fruiting body isolate strain of embodiment 1 different storage time compares with the fresh fruiting body isolate strain

[0027] From March to April, select morels that are mature, robust, free from diseases and insect pests, with full caps, fully expanded ribs and pits on the caps, milky white stipe, normal shape, and moderate volume, and make 2 parts on average, 1 part Immediately isolate the strains, and spread out the other part in a well-ventilated, low temperature (10-15°C), dark, dry place, and turn it over from time to time until the fruiting body is completely dry. Take a small amount of cap tissue, After soaking and softening in clean water, grind it in a mortar to make a tissue suspension. After microscopic examination, select the dry fruiting body with many ascospores, put it in an envelope, and store it in a dry, dark and cool place indoors, and take measures to prevent pests and rodents.

[0028]Due to microscopic examination of spore germination,...

Embodiment 2

[0036] The influence of embodiment 2 separation medium on dry spore isolation strain

[0037] In view of the previous experiments, it was found that conventional PDA was rich in nutrients, and other miscellaneous bacteria, especially bacteria, also grew in large numbers at the same time as the morel mycelium grew, and the pollution was serious. Therefore, the following experiments were performed and cultured with pure agar without any nutrients Base, wheat grain medium and conventional PDA medium were compared. Make the following 3 media:

[0038] Medium 1: conventional PDA medium, the composition and preparation method are the same as in Example 1.

[0039] Medium 2: pure agar medium, compared with conventional PDA medium, does not contain potato juice and glucose, and the rest of the ingredients and production methods are the same.

[0040] Medium 3: Wheat grain medium, select wheat grains that are free from diseases and insect pests, plump, relatively fresh, shelled and s...

Embodiment 3

[0046] Embodiment 3 The influence of culture temperature on the growth of dry spore isolate strain.

[0047] Isolate strains with dry fruiting bodies stored for 12 months, the method is the same as in Example 1, inoculate the center of the wheat grain medium, culture them in the dark at 15°C, 20°C, and 25°C respectively, 3 dishes / treatment, and record the mycelial sclerotia growing situation.

[0048] Table 3 Effects of different culture temperatures on the growth of strains isolated from dry spores

[0049]

[0050] Although the hyphae grow faster at 25°C, their growth vigor is not as good as at 20°C, and there are fewer sclerotia. Because Morchella is a low-temperature fungus, too high a temperature can easily lead to premature aging of the hyphae. Although spores can germinate at 15°C, and the growth of colonies and sclerotias is also good, but due to the slow growth of mycelium, it is easy to be contaminated by miscellaneous bacteria. Therefore, 20°C is the optimal gr...

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Abstract

The invention belongs to the technical field of microorganisms, relates to a method for preserving and separating a toadstool strain through sporocarp dried in the shade, and aims to solve the technical problems that a conventional toadstool strain preservation method is limited by season, complex to operate, easy in causing pollution and relatively high in cost. According to the technical scheme, the method for preserving and separating toadstool strain through sporocarp dried in the shade comprises the following steps: a, preserving the strain; b, separating the strain. The method provided by the invention is low in cost, simple to operate, unlimited by season, capable of effectively preserving activity of the strain, and convenient in transporting and preserving the strain.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a method for preserving and isolating hickory chick strains by drying fruiting bodies in the shade. Background technique [0002] Morchella is a rare and rare edible fungus. Although it can be cultivated artificially in the field, its output is extremely unstable, which leads to its high price. The acquisition of strains is the first step in field production of morels, and it is also a key step. At present, the separation of morel strains mainly uses fresh fruiting bodies as the separation material, and there are two specific technical routes: in the morel fruiting body output season (every March-April), take fresh fruiting bodies and hang them upside down above the culture medium , the ascospores fall naturally on the culture medium (spore separation method, commonly used); or take fresh fruit body tissue, add sterile water to grind, prepare the bacterial suspensio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04
CPCC12N1/04C12N1/14
Inventor 伍晓丽刘飞宋旭红张雪谭均崔广林莫让瑜潘媛张应孙年喜王钰陈大霞李隆云彭锐刘金亮马鹏
Owner 重庆市潼南区中药研究院有限公司
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