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Method for preserving and taking potato germplasm resources

A preservation method and potato technology, applied in botany equipment and methods, horticultural methods, applications, etc., can solve the problems of germplasm resource variation and achieve the effects of saving preservation costs, reducing the number of transfers, and reducing albinism

Pending Publication Date: 2021-03-09
宜宾市农业科学院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The addition of these plant growth inhibitors usually increases the probability of germplasm resource variation

Method used

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  • Method for preserving and taking potato germplasm resources
  • Method for preserving and taking potato germplasm resources
  • Method for preserving and taking potato germplasm resources

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A preferred embodiment of the present invention provides a method for preserving potato germplasm resources, and the specific steps are as follows:

[0029] S1. Cut the single-section section of the potato tissue culture seedling, cut it on the MS medium in the 240mL culture bottle, place 9 sections in each bottle, and seal the bottle mouth with plastic wrap;

[0030] S2. Place the potato tissue cultured seedlings treated by S1 in the cultivation room, and carry out subculture at 20-22°C, light intensity 3200lx, and light intensity 16h / day; after 2 months, only reduce the light intensity to 2300lx , and other conditions remain unchanged, continue to cultivate; after 2 months, increase the light intensity to 2700lx; Passes dormancy, begins to sprout;

[0031] S3. Inoculate the germinated test-tube tubers obtained in S2 onto the MS medium in 240mL culture bottles, place 3 capsules in each bottle, seal the bottle mouth with plastic wrap, wait for the test-tube tubers to g...

Embodiment 2

[0033] A preferred embodiment of the present invention provides a method for preserving potato germplasm resources, and the specific steps are as follows:

[0034] S1. Cut the single-section section of the potato tissue culture seedling, cut it on the MS medium in the 240mL culture bottle, place 8 sections in each bottle, and seal the bottle mouth with plastic wrap;

[0035] S2. Place the potato tissue cultured seedlings treated by S1 in the cultivation room, and carry out subculture at 20-22°C, light intensity 3500lx, and light intensity 15h / day; after 2 months, only reduce the light intensity to 2500lx , and other conditions remain unchanged, continue to cultivate; after 2 months, increase the light intensity to 3000lx; Passes dormancy, begins to sprout;

[0036] S3. Inoculate the germinated test tube tubers obtained in S2 onto the MS medium in 240mL culture bottles, place 2 capsules in each bottle, seal the bottle mouth with plastic wrap, wait for the test tube tubers to g...

Embodiment 3

[0038] A preferred embodiment of the present invention provides a method for preserving potato germplasm resources, and the specific steps are as follows:

[0039]S1. Cut the single-section section of the potato tissue culture seedling, cut it on the MS medium in the 240mL culture bottle, place 10 sections in each bottle, and seal the bottle mouth with plastic wrap;

[0040] S2. Place the potato tissue cultured seedlings treated by S1 in the cultivation room, and carry out subculture at 20-22°C, light intensity 3300lx, and light intensity 17h / day; after 2 months, only reduce the light intensity to 2400lx , other conditions remain unchanged, continue to cultivate; after 2 months, increase the light intensity to 2800lx; Passes dormancy, begins to sprout;

[0041] S3. Inoculate the germinated test-tube tubers obtained in S2 onto the MS medium in 240mL culture bottles, place 4 capsules in each bottle, seal the bottle mouth with plastic wrap, and wait for the test-tube tubers to g...

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Abstract

The invention discloses a method for preserving and taking potato germplasm resources. The method comprises the following steps that S1, single sections of potato tissue culture seedlings are shearedand cut on a culture medium in a culture bottle, and a bottle opening is sealed by using a sealing film; S2, subculture is performed on the potato tissue culture seedlings treated in step S1 at the temperature of 20-22 DEG C, the illumination intensity of 3,200-3,500 lx and the illumination time of 15-18 hours per day; after two months, the illumination intensity is reduced to 2,300-2,500 lx, andculture is continued; after two months, the illumination intensity is increased to 2,700-3,000 lx; and after two months, microtuber is naturally fruited, the illumination intensity is reduced to 0, and continuous culturing is carried out until the microtuber passes away dormancy and starts to germinate; and S3, the microtuber starting to germinate obtained in step S2 is inoculated to a culture medium in a culture bottle, a bottle opening is sealed by using a sealing film, and a new round of germplasm resource preservation is carried out according to the operation in step S2 when the microtubergrows into a new test-tube plantlet. The method for preserving and taking potato germplasm resources is simple, convenient, easy to operate, low in cost and good in effect.

Description

technical field [0001] The invention belongs to the technical field of plant germplasm preservation, and in particular relates to a method for preserving and obtaining potato germplasm resources. Background technique [0002] Potatoes, belonging to the family Solanaceae, are annual herbaceous plants with edible tubers. They are the fourth most important food crop in the world, second only to wheat, rice and corn. Potatoes are also known as yam eggs, yams, yams, yam heads, fragrant yams, yams, yams, yams, ground eggs, potatoes, etc. Potatoes have different names in different countries, such as Irish jicama in the United States, Dutch potato in Russia, ground apple in France, ground pear in Germany, ground bean in Italy, and Baba in Peru. Potato tubers contain a lot of starch, which can provide rich calories for the human body, and are rich in protein, amino acids, vitamins and minerals, especially its vitamin content is the most complete of all food crops. In European and Am...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 张德银刘兴贵廖霏霏王克秀杜勇利熊湖
Owner 宜宾市农业科学院
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