Tissue culture and rapid propagation process of Chinese Pistache

A technology for tissue culture and rapid propagation of Pistacia chinensis, applied in horticulture, cultivation, soilless cultivation, etc., can solve the problems of difficulty in obtaining sterile seedlings, easy pollution of stem segment tissue culture, high browning rate, etc., and achieve optimal culture medium Recipe, solve the effects of low reproductive efficiency and low chance of mutation

Active Publication Date: 2013-07-31
SICHUAN COLORLINK CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The germination rate of seeds is low, and it is difficult to obtain sterile seedlings; while the tissue culture of stem segments is easy to be polluted, the rate of browning is high, the germination is slow, and new shoots are easy to die, etc., it is difficult to obtain sterile seedlings

Method used

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  • Tissue culture and rapid propagation process of Chinese Pistache
  • Tissue culture and rapid propagation process of Chinese Pistache
  • Tissue culture and rapid propagation process of Chinese Pistache

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The red-leaved Pistacia chinensis tissue culture rapid propagation technique, it comprises the following steps:

[0028] A. The acquisition of sterile seedlings: collect mature seeds of Pistacia chinensis in the field, digest them with 50% sulfuric acid for 12 minutes, remove the outer testa; Then use pliers to open the seed shell on the ultra-clean workbench, and inoculate the seeds on the MS basic medium without adding any hormones. The MS medium does not add any plant growth regulators (phytohormones), First, 3d dark culture was carried out for 3 to 7 days, and then light / dark culture was carried out for 16hr / 8hr, and the temperature of the culture room was 25°C; observation records showed that after 1 month, some seeds germinated and grew into complete plants, and sterile seedlings were obtained;

[0029] B, Pistacia chinensis tissue culture rapid propagation, aseptic seedlings are carried out tissue culture rapid propagation by the segmental method, the sterile see...

Embodiment 2

[0037] The red-leaved Pistacia chinensis tissue culture rapid propagation technique, it comprises the following steps:

[0038] A. The acquisition of sterile seedlings: collect mature seeds of Pistacia chinensis in the field, digest them with 70% sulfuric acid for 12 minutes, remove the outer testa; Then use pliers to open the seed shell on the ultra-clean workbench, and inoculate the seeds on the MS basic medium without adding any hormones. The MS medium does not add any plant growth regulators (phytohormones), 3-d dark culture was carried out for 3-7 days first, followed by light / dark culture for 16hr / 8hr, the temperature of the culture room was 28°C; observation records showed that after 2 months, some seeds germinated and grew into complete plants, and sterile seedlings were obtained;

[0039] B, Pistacia chinensis tissue culture rapid propagation, aseptic seedlings are carried out tissue culture rapid propagation by segmental method, the aseptic seedlings are cut into bud...

Embodiment 3

[0048] The red-leaved Pistacia chinensis tissue culture rapid propagation technique, it comprises the following steps:

[0049] A, the acquisition of sterile seedlings, the mature seeds of Pistacia chinensis were collected in the field, digested with 60% sulfuric acid for 18 hours, and removed the outer testa; then sterilized with 70% alcohol for 3 minutes, sterilized with mercury chloride for 20 minutes, and washed with sterile water for 5 minutes Then use pliers to open the seed shell on the ultra-clean workbench, and inoculate the seeds on the MS basic medium without adding any hormones. The MS medium does not add any plant growth regulators (phytohormones), First 3d dark culture for 3 to 7 days, then light / dark culture for 16hr / 8hr, the temperature of the culture room was 26°C; observation records showed that after one and a half months, some seeds germinated and grew into complete plants, and sterile seedlings were obtained;

[0050] B, Pistacia chinensis tissue culture r...

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Abstract

The invention discloses a tissue culture and rapid propagation process of Chinese Pistache. The tissue culture and rapid propagation process of Chinese Pistache includes the steps of A, collecting ripe seeds of Chinese Pistache, and removing episperm by sulfuric acid digestion; sterilizing; inoculating the seeds to MS (Murashige and Skoog) minimal medium in which no hormone is added; B, cutting aseptic seedlings into stem segments with buds, removing part of leaves, inoculating to rooting medium or inoculating to enrichment medium prior to transferring to the rooting medium; and C, exercisingand transplanting the tissue culture seedlings. The tissue culture and rapid propagation process of Chinese Pistache has the advantages that the tissue culture method is modified, a medium formula isoptimized, and aseptic seedlings can be obtained easily and can be propagated rapidly and grow healthy and vigorous; mass high-quality test-tube seedlings can be obtained rapidly from the Chinese Pistache which is difficult or slow to propagate under natural conditions and can be applied to culture and production, and variation rate of offspring of the Chinese Pistache obtained by the process is low.

Description

technical field [0001] The invention relates to the technical field of tissue culture rapid propagation of plants, in particular to a technique for tissue culture rapid propagation of Pistacia chinensis. Background technique [0002] Pistacia chinensis (Pistacia chinensis), also known as Kaishu, etc., belongs to the genus Pistacia chinensis of the Anacardiaceae family, and is an excellent timber and ornamental deciduous tree. It is widely distributed in my country, from Hebei and Shandong in the north, to Guangdong and Guangxi in the south, to Taiwan in the east, to Sichuan and Yunnan in the west, and there are wild and cultivated Pistacia chinensis, among which Hebei, Henan, Shanxi, Shaanxi and other provinces have the most. Pistacia chinensis has a wide range of uses, with a seed oil content of 42.5% and an oil yield of 20.0% to 30.0%. It has been designated by the State Forestry Administration as one of the key forestry biomass energy tree species to be developed during t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00A01G31/00
Inventor 吴燚马建华冯爱云
Owner SICHUAN COLORLINK CO LTD
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