A kind of method for tissue culture rapid propagation of dendrobium nobile

A high-speed technology of Dendrobium nobile, which is applied in the field of tissue culture and rapid propagation of Dendrobium nobile, can solve the problems of long cultivation period, difficult large-scale and standardized production of Dendrobium nobile, and achieve low cost, reduced drug consumption and high output Effect

Active Publication Date: 2016-04-20
FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In order to solve the problems that it is difficult to carry out large-scale and standardized production of Dendrobium nobile in the prior art, and the cultivation period is long, the present invention provides a method for tissue culture rapid propagation of Dendrobium nobile, which adopts tissue culture technology to propagate Dendrobium nodrobii, Tissue culture of Dendrobium candidum through protocorm can obtain a large number of seedlings, which can not only cultivate a large number of seedlings of Dendrobium with consistent traits in a short period of time, meet market demand, but also play a very important role in the protection of wild resources of Dendrobium. the meaning of

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] (1) Sterilize the self-dehiscated mature seeds of Dendrobium nobile with 3% hydrogen peroxide for 10 minutes, blot the surface liquid, and then inoculate the seeds in the following protocorm induction medium:

[0047] MS medium

[0048] 6-Benzylaminopurine 6-BA0.2mg / L

[0049] Naphthaleneacetic acid NAA0.1mg / L

[0050] Sucrose 30000mg / L

[0051] Agar 4500mg / L

[0052] Banana 50000mg / L

[0053] Potatoes 25000mg / L

[0054] pH5.8,

[0055] Under the condition that the light intensity is 1500lx, the temperature is 27°C, and the light time is 10h / d, cultivate for 20 days to obtain enlarged protocorms, and continue to cultivate for 55 days to differentiate the protocorms into seedlings;

[0056] (2) Transfer the seedlings obtained in step (1) to the following proliferation medium:

[0057] 1 / 2MS medium

[0058] 6-Benzylaminopurine 6-BA0.05mg / L

[0059] Naphthaleneacetic acid NAA0.2mg / L

[0060] Sucrose 30000mg / L

[0061] Agar 4500mg / L

[0062] Banana 50000mg / L

...

Embodiment 2

[0079] (1) Sterilize the self-dehiscated mature seeds of Dendrobium nobile with 3% hydrogen peroxide for 13 minutes, blot the surface liquid, and then inoculate the seeds in the following protocorm induction medium:

[0080] MS medium

[0081] 6-Benzylaminopurine 6-BA0.5mg / L

[0082] Naphthaleneacetic acid NAA0.05~0.5mg / L

[0083] Sucrose 30000mg / L

[0084] Agar 4500mg / L

[0085] Banana 50000mg / L

[0086] Potatoes 25000mg / L

[0087] pH5.9

[0088] Under the condition that the light intensity is 1800lx, the temperature is 25°C, and the light time is 11h / d, cultivate for 25 days to obtain enlarged protocorms, and continue to cultivate for 50 days to differentiate the protocorms into seedlings;

[0089] (2) Transfer the seedlings obtained in step (1) to the following proliferation medium:

[0090] 1 / 2MS medium

[0091] 6-Benzylaminopurine 6-BA0.01mg / L

[0092] Naphthaleneacetic acid NAA0.5mg / L

[0093] Sucrose 30000mg / L

[0094] Agar 4500mg / L

[0095] Banana 50000mg / L...

Embodiment 3

[0112] (1) Sterilize the self-dehiscated mature seeds of Dendrobium nobile with 3% hydrogen peroxide for 15 minutes, blot the surface liquid, and then inoculate the seeds in the following protocorm induction medium:

[0113] MS medium

[0114] 6-Benzylaminopurine 6-BA0.01mg / L

[0115] Naphthaleneacetic acid NAA0.05mg / L

[0116] Sucrose 30000mg / L

[0117] Agar 4500mg / L

[0118] Banana 50000mg / L

[0119] Potatoes 25000mg / L

[0120] pH6.0,

[0121] Under the condition that the light intensity is 2000lx, the temperature is 27°C, and the light time is 12h / d, cultivate for 30 days to obtain enlarged protocorms, and continue to cultivate for 60 days to differentiate the protocorms into seedlings;

[0122] (2) Transfer the seedlings obtained in step (1) to the following proliferation medium:

[0123] 1 / 2MS medium

[0124] 6-Benzylaminopurine 6-BA0.5mg / L

[0125] Naphthaleneacetic acid NAA0.05mg / L

[0126] Sucrose 30000mg / L

[0127] Agar 4500mg / L

[0128] Banana 50000mg / L ...

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Abstract

A provided dendrobium aphyllum tissue-culture quick propagation method comprises: germination of cracked mature seeds obtained by inbred of dendrobium aphyllum, forming and differentiation of protocorms, rooting culture, culture of complete plants, transplanting and surviving. Compared with conventional propagation methods such as high-position bud cutting or strain segregating, the method provided by the invention employs tissue culture technology for propagation of dendrobium aphyllum, and the tissue culture of dendrobium aphyllum is performed by employing protocorms, so that the propagation speed is fast, a large number of seedlings are obtained and grow well, and thus a large number of dendrobium aphyllum seedlings with same properties are cultured in a short period, the transplanting survival rate is high, the market demand is satisfied, and the method has various advantages such as low cost, short period, high yield and the like.

Description

technical field [0001] The invention relates to a method for tissue culture and rapid propagation of Dendrobium nobile, belonging to the technical field of plant tissue culture. Background technique [0002] Dendrobium dendrobium (Den. aphyllum (Roxb.) C.E. Fischer), also known as Dendrobium tiangong and Dendrobium inverted spring, is a perennial epiphytic herb of the genus Dendrobium orchidaceae. On tree trunks in leaf forests or on rocks under sparse forests, it is mainly distributed in Yunnan, my country, India, Myanmar, Thailand, Laos, Vietnam, Malaysia and other countries. It is not only an important ornamental plant, but also one of the important original plants of the commercial Chinese medicinal material Dendrobium. It is used as medicine with fresh stems or dried stems. It is one of the types of Dendrobium commonly used in folk medicine in my country. Dendrobium candidum clears heat and troubles, clears liver and calms wind, diuresis and detoxification. Treat polyd...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 曹桦李绅崇杨春梅王继华汪国鲜吴丽芳赵培飞单芹丽阮继伟许凤
Owner FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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