Rice disease-resistant gene RGA5-HMA5 and application thereof in rice breeding

Abstract

The invention discloses a rice disease-resistant gene RGA5-HMA5 and application thereof in rice breeding. On the basis of an RGA5 gene, a heavy metal-associated (HMA) structural domain at a carbon endof protein encoded by the RGA5 is subjected to point mutation to obtain the RGA5 gene mutant RGA5-HMA5. Experiments indicate that the protein encoded by the RGA5-HMA5 can specifically recognize effector protein AVR-Pik of magnaporthe oryzae to relieve the inhabitation function of the effector protein AVR-Pik on an RGA4 gene and activate immunoreactions of rice, and recognition of AVR-Pik effectorprotein is changed, so that rice containing the disease resistant gene obtains resistance to magnaporthe oryzae containing the AVR-Pik effector protein. The rice disease-resistant gene RGA5-HMA5 hasimportant significance in rice blast resistant genetic improvement and disease-resistant variety breeding of rice.

Description

technical field [0001] The invention relates to a rice disease resistance gene RGA5-HMA5 and its application in rice breeding. Background technique [0002] As one of the most important food crops in the world, rice provides food sources for more than 50% of the global population. However, rice production is often threatened by various diseases, among which rice blast caused by Magnaporthe oryzae is one of the most important diseases affecting rice production. Rice blast causes about 20-30% of the total rice output to be lost every year. Plants have an efficient endogenous immune system to resist the infection of various pathogens, which relies on the specific recognition of effector proteins secreted by pathogenic bacteria by anti-disease proteins. This recognition often triggers localized programmed cell death, also known as a hypersensitive response (HR). Generally, NB-LRR-type disease resistance proteins containing nucleic acid-binding and leucine repeat domains can s...

AI Technical Summary

Problems solved by technology

Although the occurrence of rice blast can be effectively controlled by mixing different rice varieties in the field, this strategy still has certain limitations in the wide application of agricultural production.

For example, when different varieties are mixed and planted, the mixing ratio of the varieties changes with the change of the growing season, and farmers need to re-plant the mixed varieties in the new growing season, which is time-consuming and laborious

In addition, when rice varieties with different agronomic characteristics are mixed, it will often cause harvest difficulties. For example, the mixed planting of early-maturing varieties and late-maturing varieties will prolong the harvest period and increase the consumption of manpower and material resources.

Mixing different varieties is also not conducive to field management, and there are difficulties in adjusting field planting density, fertilization, weeding, etc.

The mixed planting of different varieties makes the quality of the harvested agricultural products uneven, which affects sales

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