20results about How to "Activate immune response" patented technology

The invention belongs to the technical field of biomedical treatment, and discloses a preparation method of immunomodulatory recombinant macrophage vesicles, and the preparation method specifically comprises the following steps: step 1, clinical sequencing of gingival tumor tissues and pathogenic microbiota data analysis; hybridizing and fusing a macrophage membrane infected by the porphyromonas gingivalis and an exosome membrane; and 3, constructing a piezoelectric driven ROS (reactive oxygen species) to generate the immunoregulation vesicles. The invention provides an innovative nano vesicle technology, and the technology shows double advantages in tumor treatment: firstly, accurate targeting and efficient killing of key porphyromonas gingivalis in a tumor environment are realized by constructing recombinant macrophage vesicles, and meanwhile, the stability of normal flora is kept, and tumor deterioration is prevented; and secondly, the anti-tumor immune response is obviously activated by utilizing the immunoregulation capability of the bacteria-induced macrophages and exosome vesicles thereof, including converting immunosuppressed M2 type macrophages into anti-tumor M1 type macrophages, so that the growth of solid tumors is effectively inhibited.

The invention relates to a citric acid-based particle injection material as well as a preparation method and application thereof. The preparation method comprises the following steps: melting and heating citric acid and 1, 8-octylene glycol to form a POC prepolymer, dissolving the POC prepolymer with an organic solvent to obtain a dissolved solution, and slowly pouring the dissolved solution into ultrapure water for multiple times for purification to obtain a purified POC degradable prepolymer; and dissolving the POC degradable prepolymer with a solvent, adding inorganic salt particles, and carrying out a cross-linking reaction to obtain the POC injectable particle material. Medicines, active molecules, various metal ions and the like are loaded on POC in a physical compounding or chemical crosslinking mode, and the citric acid-based particle injection material is obtained. The citric acid-based biological material provided by the invention, as a novel medical beauty raw material and a related particle injection filler thereof, can effectively promote regeneration, proliferation, migration and chemotaxis of skin cells, promote the production of collagen and achieve a medical beauty effect. Meanwhile, the immune response of the organism can be activated, inflammation is reduced, the product after degradation is citric acid which can be metabolized randomly, and obvious immunogenicity and toxic and side effects are avoided.

The invention relates to a multi-antigen monkey pox RNA vaccine and a preparation method thereof, and relates to the technical field of nucleic acid vaccines. The multi-antigen mRNA comprises a sequence encoding an antigenic polypeptide of the monkey pox virus or an antigenic fragment, variant or derivative thereof, the antigenic polypeptide or the antigenic fragment thereof comprising at least viral proteins of an intracellular mature virus and an extracellular envelope virus of the monkey pox virus. The invention provides a multi-antigen mRNA vaccine aiming at monkey pox viruses and a sequence, mRNA can express antigen protein in in-vitro cells, high-titer anti-monkey pox virus A29L, A33L, B6R, M1R and E8L protein IgG can be detected after a mouse is immunized, a high-titer anti-vaccinia Tian Tan strain live virus neutralizing antibody is generated, good immunogenicity is achieved, and the vaccine can be used for preparing the multi-antigen mRNA vaccine aiming at the monkey pox viruses. The important significance is realized on the prevention of the monkey pox virus.

The present invention relates to a drug carrier based on bacterial cell membrane and its preparation method and application. The nano drug composition of the present invention includes a drug and a bacterial cell membrane drug-carrying system, wherein the bacterial cell membrane drug-carrying system encapsulates the drug; the bacterial cell membrane drug-carrying system includes a bacterial cell membrane and a liposome; the bacterial cell membrane is wrapped outside the liposome; it can be efficiently enriched in the tumor site, release a drug that can kill Fusobacterium nucleatum, effectively alleviate the immunosuppressive microenvironment caused by Fusobacterium nucleatum, restore the response of colon cancer to anti-cytotoxic T lymphocyte-associated antigen 4 therapy and anti-programmed death receptor therapy, and can also effectively alleviate the resistance of Fusobacterium nucleatum to chemotherapy drugs, and restore the responsiveness of the tumor to the chemotherapy drug 5-fluorouracil. The nano drug composition can also kill a variety of bacteria in the breast cancer tumor site to alleviate tumor metastasis caused by tumor bacteria, and restore the responsiveness of the tumor to the combined chemotherapy regimen.

The application discloses a CD40L-IL-2 fusion protein and a preparation method and application thereof in preparing a tumor treatment drug. The fusion protein comprises a CD40L trimer or a variant thereof and a cytokine IL-2 or a variant thereof; the CD40L trimer comprises three CD40L monomers connected. The fusion protein provided by the application can synergistically activate APC-dependent antigen presentation and T cell-mediated immune killing, forms a closed-loop anti-tumor immune response of 'antigen presentation-immune initiation-effect amplification', and provides an effective strategy for treating tumors.

The invention relates to the field of A61K8 / 18, in particular to a skin care target-based micro-ecological composition with soothing and repairing effects, which is at least prepared from the following raw materials in parts by weight: 1 to 40 parts of prebiotics, 0 to 20 parts of probiotics, 0.1 to 3 parts of auxiliaries and 50 to 100 parts of solvent, wherein the prebiotics at least comprise at least one of dextran, ribose, inulin and ectoine, the microecological balance of the skin is effectively regulated, meanwhile, the effects of repairing the skin barrier, relieving redness, stabbing pain and itching, resisting oxidation and the like are achieved, and the use requirements of the sensitive skin are better met.

The application discloses application of an immune elicitor protein FvXynC and a protein combination in plant disease resistance, and belongs to the technical field of plant immune elicitors. A new immune elicitor protein FvXynC is found from F. pseudograminearum, and the immune elicitor protein has the core functions of activating plant immune response and improving plant resistance to F. pseudograminearum, can be used as an efficient plant immune inducer, provides a new technical path for plant disease resistance improvement and green plant disease control, and has important agricultural application value. The FvXynC and the immune elicitor protein FvGH43A are compounded, so that the resistance level of corn to F. pseudograminearum can be synergistically improved, and the problem that the plant disease control effect of a single immune elicitor protein is limited is solved.

The present invention relates to the field of microorganisms, and specifically to providing a new functional lactic acid bacteria expressing ETEC antigens, and a preparation method and application thereof. The recombinant Lactobacillus plantarum expressing ETEC antigens uses Lactobacillus plantarum NC8 as a host bacterium and a pPG612.1 expression vector, wherein the pPG612.1 expression vector contains the FaeC gene or LngC gene of ETEC. The present invention innovatively integrates the FaeC and LngC genes of ETEC into Lactobacillus plantarum NC8 for expression, and can activate the intestinal mucosal immune response by oral administration, which can not only directly counteract the intestinal damage caused by ETEC and protect the intestinal villus structure, but also can regulate the intestinal microenvironment, stimulate the body to produce a systemic immune response, improve the body's ability to resist ETEC infection, reduce the morbidity and mortality of ETEC infection, and thus improve the economic benefits of the pig farming industry.

The present invention relates to the biological field, and in particular to the application of tomato creeping mold fungal virus coat protein SlAV1-CP in improving plant defense capabilities. The tomato creeping mold fungal virus coat protein SlAV1-CP, the encoding gene SlAV1-CP or the biological material containing the encoding gene SlAV1-CP provided by the present invention can activate plant immune response, increase the expression amount of defense-related genes in plants, thereby inducing plants to produce disease resistance, reduce the infection of pathogens, and provide a new way to improve plant resistance. At the same time, it also provides new materials for the research and development of new plant immune inducers (plant immune activators). Moreover, the protein has been verified in a variety of plants, and all have the effect of improving plant disease resistance, so it can be applied to the prevention and treatment of a variety of plant diseases.

This invention discloses a type B influenza virus T4 peptide that can induce cellular immune responses and its applications, belonging to the fields of immunotherapy and immunoassay technology. The immunogenicity of this long peptide (SEQ ID NO.1, named T4) was verified in 21 volunteers using ELISpot assay. Results showed that T4 was positive in 5 individuals, with a positive rate of 23.81%. ELISpot assay of human PBMCs (IFN-γ secretory cells) showed that T4 induced an immune response of up to 3060 SFCs / 10 in the population. 6 PBMC. Therefore, this peptide has good immunogenicity and universality in the population, and can be used as a specific detection peptide for monitoring cellular immune levels. At the same time, this peptide can effectively activate the T cell immune response in the population, and has the potential to be used as a candidate vaccine epitope peptide for the design of novel vaccines.

The present invention provides a fusion protein vaccine composition which identifies an immunocompetent domain (domain, site or fragment) in an Rv2299c protein, and removes unwanted sites or selects only desired moieties to link to other immunocompetent proteins or sites, thereby effectively activating an immune response in a tuberculosis patient, improving the immunocompetent activity of the Rv2299c protein, and improving the immunocompetent activity of the Rv2299c protein and the immunocompetent activity of the Rv2299c protein in the Rv2299c protein in the Rv2299c protein in the Rv2299c protein in the Rv2299c protein. And an epoch-making contribution is made to prevention or treatment of tuberculosis.

This invention discloses the preparation and application of radiofrequency thermoresponsive platinum ion crosslinked black phosphorus nanogel. The nanogel has a core-shell structure, comprising a core composed of a radiofrequency thermosensitive agent and chemotherapeutic drugs, and a shell constructed from a thermosensitive nanopolymer. The thermosensitive nanogel BP-Pt@PNA prepared using the above components exhibits radiofrequency thermosensitive properties. Simultaneously, the introduction of the thermosensitive nanopolymer improves the stability of the radiofrequency thermosensitive agent and chemotherapeutic drugs, enabling long-term drug retention and fully leveraging the synergistic effect of non-invasive radiofrequency thermotherapy and chemotherapy. This drug-loaded nanogel can achieve multimodal treatment applications, including non-invasive radiofrequency thermotherapy-chemotherapy synergistic antitumor therapy and invasive radiofrequency ablation-chemotherapy-vascular embolization synergistic antitumor therapy, demonstrating significant clinical translational potential.

The application belongs to the technical field of biological medicine, and particularly relates to a Brucella recombinant multi-epitope vaccine and application thereof. The application provides a Brucella recombinant multi-epitope vaccine protein, and the vaccine protein is a first vaccine variant rBMEV1 or a second vaccine variant rBMEV2. The results of examples prove that the vaccine provided by the application can stimulate a strong cross immune response against main pathogenic species (such as sheep species, bovine species and porcine species) of Brucella. Animal tests prove that the vaccine can induce high-level and long-lasting specific antibodies, and significantly activate CD4 + and CD8 + T cells and corresponding cytokine responses, and has good safety. The application solves the problems of a narrow protection spectrum, a dependence on exogenous adjuvants and a single immune type of a traditional Brucella vaccine, and provides a new generation of high-efficiency, broad-spectrum and safe candidate vaccine strategy for the prevention and control of brucellosis.

The invention discloses a multifunctional coordination polymer for activating a thermal therapy induced in-situ vaccine as well as a preparation method and application of the multifunctional coordination polymer, a blood vessel disrupting agent, a photosensitizer and a contrast agent Gd3 + are constructed and integrated, the blood vessel disrupting agent is used for opening a tumor blood vessel barrier and increasing permeation of a photosensitive drug, and the photosensitizer and tumor bleeding further improve the photo-thermal effect; according to the present invention, the photothermal anti-tumor immune response is enhanced, the Gd < 3 + >-mediated nuclear magnetic imaging is cooperated to achieve the precise positioning treatment on the tumor, the synergistic interaction effect is achieved, the tumor immunotherapy is enhanced, the solubility of the indissolvable drug can be improved, the toxicity can be reduced, the delivery efficiency of the molecular targeting drug can be improved, and the further combination with the anti-PD-1 immunotherapy can be achieved; therefore, the compound has a promising application prospect in preparation of anti-tumor drugs.

The application discloses a postoperative auxiliary comprehensive treatment system with spatiotemporal response and a preparation method and application thereof, and relates to the fields of biology and medicine and the technical field of pharmaceutical preparations. The system comprises a temperature-sensitive hydrogel matrix, an anti-inflammatory drug dispersed in the hydrogel matrix, and nanoparticles loaded with an immunomodulator and dispersed in the hydrogel matrix. In an embodiment of the application, a postoperative auxiliary treatment comprehensive system is successfully constructed, and curcumin and MPDA encapsulating R848 are simultaneously loaded in the gel support. The small molecule curcumin is preferentially released from the gel to play an anti-inflammatory role, and R848 needs to be gradually released from the mesopore of the MPDA and the gel, so that the immune microenvironment can be improved after the anti-inflammatory curcumin promotes wound healing.

The invention discloses an HLA-A * 24: 02 renal cell carcinoma tumor antigen oligopeptide derived from a human retrovirus HERV3895 gene and a preparation method of the HLA-A * 24: 02 renal cell carcinoma tumor antigen oligopeptide. The invention provides a tumor antigen peptide. The amino acid sequence of the tumor antigen peptide is as shown in SEQ ID No. 1. Researches find that a compound formed by the oligopeptide and a corresponding HLA molecule (HLA-A * 24: 02) can be recognized by T cells, and immune response is activated. The invention provides a potential target spot for treatment of renal cell carcinoma, and provides a new target spot screening direction for subsequent tumor antigen treatment of renal carcinoma.

This invention relates to the field of traditional Chinese medicine cultivation technology, specifically the application of D6-abelacterol, and particularly its application in promoting the growth of Panax notoginseng, inducing resistance, and improving its quality. Experiments have shown that D6-abelacterol significantly enhances the resistance of Panax notoginseng to pathogens such as black spot disease, improving its disease resistance; simultaneously, it promotes plant growth, significantly increasing the yield and quality of Panax notoginseng, exhibiting a significant growth-promoting effect. The D6-abelacterol provided by this invention is a natural product, possessing environmentally friendly, low-toxicity, and green properties, laying the foundation for the development of the Panax notoginseng industry.

This invention discloses the application of PACAP peptides as plant immunomodulators, belonging to the field of plant immunology technology. This invention is the first to apply PACAP to a plant system, discovering that it can act as a novel plant immunomodulator to regulate plant immune responses, thereby enhancing plant resistance to pathogenic microorganisms. Experimental results show that PACAP can rapidly induce intracellular calcium ion influx in Arabidopsis thaliana cells, activate the MAPK signaling pathway, and upregulate the expression of early immune marker genes such as FRK1. Furthermore, PACAP treatment significantly improves plant resistance to pathogens such as *Pseudomonas syringae* and *Ralstonia solanacearum*. This invention also confirms that not only does the full-length PACAP (1-38) possess plant immune-promoting activity, but its N-terminal truncated peptide (1-27) and receptor antagonist fragment (6-38) also have plant immune-inducing functions. This invention provides a new technical solution for the development of plant immune inducers.

The invention provides a weak alkaline sodium salt nano drug, a preparation method and an application thereof, which is prepared by a sodium oleate and ammonium bicarbonate microemulsion method. The weak alkaline sodium salt nano drug prepared by a sodium oleate and ammonium bicarbonate microemulsion method, namely NaHCO 3 , which can not only neutralize lactic acid in the tumor microenvironment, consume lactic acid, and relieve the immunosuppressive tumor microenvironment; at the same time, it can degrade and release a large number of ions in tumor cells, induce cell pyroptosis, and activate immune response; thus, through the synergistic effect of the two aspects, the systemic anti-tumor immune response is activated to fight against tumor recurrence and metastasis. The preparation method of the weakly alkaline sodium salt nanomedicine provided by the present invention is simple, the reaction conditions are mild, the raw material cost is low, and it has great application prospects.

The invention belongs to the field, and discloses a nasal spray WT1 polypeptide preparation, a preparation method, a use method and application, the preparation contains a polypeptide fragment of a specific WT1 antigen, through nasal spray administration, the vaccine can rapidly stimulate local and whole body immune response, and therefore the effect of preventing various tumors is achieved. The vaccine preparation disclosed by the invention has the advantages of local administration, quick effect, strong targeting property, easiness in storage and transportation and the like, and has a good application prospect.