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Anti-disease gene OsRLR1, transcription factor OsWRKY19 and application of anti-disease gene OsRLR1 and transcription factor OsWRKY19 in bacterial leaf blight resistant breeding of rice

A technology of bacterial blight resistance and disease resistance gene, applied in the field of molecular biology, can solve the problems of serious environmental protection and drug resistance, unstable control effect, difficult to popularize and apply, etc.

Active Publication Date: 2020-05-22
SOUTHWEST UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] At present, the control of rice bacterial blight mainly relies on traditional chemical agents, but the problems of environmental protection and drug resistance are becoming more and more serious
The emerging biological control is difficult to be popularized and applied due to the difficulty in screening antagonistic microorganisms, unstable control effect and high cost (Chen Lihua et al., 2014; Liu Wei, Yang Chao, Zou Jianfeng & Song Juan, 2009)
The current research has excavated some QTL loci for bacterial blight resistance, but there is little research on the molecular mechanism of rice bacterial blight resistance

Method used

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  • Anti-disease gene OsRLR1, transcription factor OsWRKY19 and application of anti-disease gene OsRLR1 and transcription factor OsWRKY19 in bacterial leaf blight resistant breeding of rice
  • Anti-disease gene OsRLR1, transcription factor OsWRKY19 and application of anti-disease gene OsRLR1 and transcription factor OsWRKY19 in bacterial leaf blight resistant breeding of rice
  • Anti-disease gene OsRLR1, transcription factor OsWRKY19 and application of anti-disease gene OsRLR1 and transcription factor OsWRKY19 in bacterial leaf blight resistant breeding of rice

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1, analysis of the resistance of rice wild type and mutant rlr1 to bacterial blight

[0038] Using the mutant rlr1 of the mutant gene OsRLR1 (LOC_Os10g07978) under the genetic background of Jinhui 10 and Jinhui 10 as wild-type controls, bacterial blight (zhe173, provided by the China Rice Research Institute) was inoculated at the tillering stage. It was found that the lesion length of the wild type was much higher than that of the mutant rlr1( figure 1 Middle A). The lesion length statistics were carried out on the 5th, 10th, and 15th days after inoculation with bacterial blight, and the statistical results also showed that: the lesion length of the wild type was constantly becoming longer as time went on, while the diseased spot length of the mutant rlr1 The plaque length is not only much smaller than that of the wild type, but also changes little ( figure 1 Middle B). And in the 15th day after the bacterial blight inoculation, the biomass statistics of the ...

Embodiment 2

[0040] Example 2. Overexpression of OsRLR1 enhances resistance of rice to bacterial blight

[0041] Transgenic plants overexpressing OsRLR1 under the genetic background of Jinhui 10 (a popularized variety) were used to inoculate bacterial blight. The results showed that compared with the wild type, the leaf lesion length of overexpressing OsRLR1 plants was shorter ( figure 2 Middle A). The statistical results at 5, 10 and 15 days after inoculation also showed that the lesion length of the overexpressing OsRLR1 plants was shorter than that of the wild type ( figure 2 Middle B). Simultaneously, the biomass statistical result of bacterial blight in the leaves of 15 days after inoculation also shows: the bacterial blight in the leaves of overexpression plants is less ( figure 2 Middle C). Therefore, the disease resistance gene OsRLR1 can enhance the resistance of rice to bacterial blight.

Embodiment 3

[0042] Example 3, OsRLR1 interacts with transcription factor OsWRKY19

[0043] In view of the fact that the genes of the CLR family usually play a defensive role by interacting with transcription factors of the WRKY family through their CC domains, five WRKY genes were selected: OsWRKY13 (LOC_Os01g54600), OsWRKY19 (LOC_Os05g49620), OsWRKY47 (LOC_Os07g48260), OsWRKY604 (LOC_Os604 ) and OsWRKY76 (LOC_Os09g25060), screened with the CC domain of OsRLR1 (OsRLR1 CC ) interacting proteins. Since full-length WRKY proteins are generally toxic in yeast (Inoue et al., 2013), a truncated protein (carboxyl group removed) was used in the present invention. The co-transformed yeast could grow on DDO medium ( image 3 Middle A), but only OsWRKY19 can interact with OsRLR1 CC grow on the deficient medium QDO / AbA ( image 3 middle A right). To further verify our experimental results, truncated OsRLR1 and OsRLR1 (E318V) and two full-length (FL) proteins of the wild-type and mutant types were...

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Abstract

The invention relates to an anti-disease gene OsRLR1, a transcription factor OsWRKY19 and an application of the anti-disease gene OsRLR1 and the transcription factor OsWRKY19 in bacterial leaf blightresistant breeding of rice. Molecular biology and a biochemical technique show that overexpression of OsRLR1 enhances the resistance to bacterial leaf blight. The anti-disease gene OsRLR1 and the transcription factor OsWRKY19 have mutual effect on protein and can enhance the resistant reaction to the bacterial leaf blight. Mutation of the rice anti-disease gene OsRLR1 causes brownness of disease like spots of leaves, expression of related genes of disease course of rice is enhanced, immunoreaction in a body is activated, and the resistance to the bacterial leaf blight is enhanced. A new way isdeveloped for enhancing the bacterial leaf blight resistance of rice and improving the yield of the rice.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and relates to gene OsRLR1, transcription factor OsWRKY19 and their application in rice bacterial blight resistance breeding. Background technique [0002] At present, the control of rice bacterial blight mainly relies on traditional chemical agents, but the problems of environmental protection and drug resistance are becoming more and more serious. The emerging biological control is difficult to be promoted and applied due to the difficulty in screening antagonistic microorganisms, unstable control effect and high cost (Chen Lihua et al., 2014; Liu Wei, Yang Chao, Zou Jianfeng & Song Juan, 2009). The current research has excavated some QTL loci for bacterial blight resistance, but there is little research on the molecular mechanism of rice bacterial blight resistance. Therefore, rice bacterial blight resistance genes were screened and applied to molecular breeding to produce high-yiel...

Claims

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Application Information

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IPC IPC(8): C12N15/29C07K14/415C12N15/82A01H5/00A01H6/46
CPCC07K14/415C12N15/8281
Inventor 何光华杜丹桑贤春张长伟李云峰凌英华杨正林邢亚迪鲁欣蔡林军员菡张秋丽张莹莹陈新龙刘明明
Owner SOUTHWEST UNIVERSITY
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