5 results about "Attenuated Bacteria" patented technology

Described herein are recombinant, attenuated bacteria with an altered quorum sensing pathway. The recombinant bacteria are used for visualizing quorum sensing or immunizing marine organisms against infections by Vibrio spp.

This invention discloses the application of a glutamine-depleting engineered bacterium and its pharmaceutical composition. The engineered bacteria use attenuated bacteria with tumor-targeting capabilities as vector strains. The genome or plasmid of the vector strain integrates a therapeutic gene encoding glutaminase. This method achieves deep and continuous depletion of glutamine in the tumor, avoiding drug resistance caused by tumor metabolic compensation. The inhibition rate against c-Myc-overexpressing soft tissue sarcomas is significantly higher than that of small molecule inhibitors. Enzyme production is only carried out locally in the tumor, with no effect on normal tissues and no systemic toxicity. The engineered bacteria can penetrate the dense tumor matrix and colonize the tumor, solving the problem of poor tissue penetration of small molecule inhibitors. The engineered bacteria continuously multiply and produce enzymes in the tumor, achieving continuous release of glutaminase. The half-life is significantly longer than that of recombinant enzymes, eliminating the need for frequent dosing. The glutaminase optimized by protein engineering has significantly reduced immunogenicity.

The present invention relates to live attenuated bacteria which persist in a subject and their use in vaccine compositions. In particular, the invention is directed to mutant strain E.coli vaccines which are useful for poultry, and especially chickens.

The invention discloses an engineered immune cell as well as a preparation method and application thereof, the engineered immune cell is transformed through genetic engineering, and (1) a Gasdermin D N-terminal fragment (GSDMD-N) expression cassette controlled by a heat shock protein HSP70 promoter is stably integrated in a genome; (2) a photothermal conversion material and / or magnetic nanoparticles used for exogenous stimulation heating are loaded in the cells; and (3) attenuated bacteria and / or anti-tumor drugs for therapeutic loading; wherein the nucleotide sequence of the HSP70 promoter is as shown in SEQ ID NO: 1, and the nucleotide sequence of the GSDMD-N is as shown in SEQ ID NO: 2. According to the invention, accurate and controllable deployment of anti-cancer drugs such as bacteria loaded in immune cells at tumor sites is realized, good anti-tumor effect and high safety are achieved, and a new technical platform is provided for cell therapy and living microorganism anti-tumor therapy.

Described herein are recombinant, attenuated bacteria with an altered quorum sensing pathway. The recombinant bacteria are used for visualizing quorum sensing or immunizing marine organisms against infections by Vibrio spp.