Kit for activating colorectal cancer specific immunity response

A technology for colorectal cancer and immune response, applied to blood/immune system cells, medical preparations containing active ingredients, animal cells, etc.

Inactive Publication Date: 2016-01-06
SHANGHAI LONGYAO BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, DC1 cells highly expressing IL12 loaded with tumor-specific antigen peptides can provide a strategy for enhancing the efficacy of antig...

Method used

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  • Kit for activating colorectal cancer specific immunity response
  • Kit for activating colorectal cancer specific immunity response
  • Kit for activating colorectal cancer specific immunity response

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1 (cell culture supernatant is T cell culture supernatant)

[0033] When the kit of the present invention is used to activate the specific immune response of colorectal cancer patients, the preparation of type 1 polarized dendritic cells (type1 polarized dendritic cells, DC1) derived from adult peripheral blood is firstly performed. Peripheral blood mononuclear cells were isolated according to the method described by Jonuleit et al. (GeneTher. 200310(3)). First, peripheral blood mononuclear cells were separated by Ficoll-Hypaque density gradient centrifugation, and plasma was collected for subsequent culture. By density 3.0×10 6 / ml, the cells were inoculated into T75 culture flasks, and 10% plasma was added to the GGT551 medium, placed at 37.0°C, saturated humidity, 5% CO 2 cultivated in the environment. After culturing for 3 hours, the suspended lymphocytes were washed away, and DMEM / F12 medium containing GM-CSF and IL-4 1000 IU / ml and 10% plasma was add...

Embodiment 2

[0038] Embodiment two (cell culture supernatant is NK cell and K562 cell co-culture supernatant)

[0039] Peripheral blood mononuclear cells were isolated according to the method described by Jonuleit et al. (GeneTher. 200310(3)). First, peripheral blood mononuclear cells were separated by Ficoll-Hypaque density gradient centrifugation, and plasma was collected for subsequent culture. IL-2 (2000U / ml) and IL15 (2ng / ml) were added. Doubling the medium every two to three days, and supplementing the factors in equal amounts. Collected on the twelfth day. After the induction is completed, NK cells are treated by 1-3×10 6 / ml was inoculated into medium RPMI1640, IFNa (1000UI / ml) was added and 0.5-1.5×10 5 Inoculate K562 cells (can be replaced with K562-NK cells) at a density of / ml for mixed culture, collect the medium supernatant during 24-48 hours of activation, filter and freeze at -80 degrees or directly use it for the preparation of DC1 cells. Peripheral blood mononuclear ...

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Abstract

The invention provides a kit for activating colorectal cancer specific immunity response. The kit comprises an RPMI-1640 culture medium, a cell culture supernatant, a GM-CSF, an IL-4, an INF gamma and a colorectal cancer specific antigenic peptide combination. The colorectal cancer specific antigenic peptide combination includes MAGE-A3-A2 antigenic peptides, MAGE-A4-A2/3 antigenic peptides, SSX-2-A2 antigenic peptides, PLAC1-A2 antigenic peptides, hTERT-A2 antigenic peptides, hTERT-A3 antigenic peptides, HER2-A2 antigenic peptides, HER2-A3 antigenic peptides, Survivin-A2 antigenic peptides, Survivin-A3 antigenic peptides, COX-2-A2/3 antigenic peptides and MTA1-A2 antigenic peptides. The kit can efficiently activate colorectal cancer specific immunity response in an active inducing/passive inducing combined mode.

Description

technical field [0001] The invention relates to a kit for enhancing anti-cancer immunity, in particular to a kit for activating colorectal cancer specific immune response. Background technique [0002] Colorectal cancer is a common malignant tumor, including colon cancer and rectal cancer. Since entering the 21st century, great advances in molecular biology and immunology have provided new strategies for clinical tumor treatment. A large number of tumor cell-specific antigens have been identified by means of molecular biology, and their recognition sites by immune cells have been analyzed by means of bioinformatics, which brings the possibility of rebuilding the patient's tumor-specific immune response . However, the results of a series of clinical trials in the past ten years have shown that the clinical effect of tumor vaccines based on tumor-specific antigen peptides is disappointing, and one of the important reasons is the defect in the antigen presentation process. ...

Claims

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Application Information

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IPC IPC(8): C12N5/0784C12N5/0783A61K39/00A61P35/00
Inventor 李伟叶圣勤汪鑫瞿苏
Owner SHANGHAI LONGYAO BIOTECH CO LTD
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