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Swine fever and porcine pseudorabies virus bivalent subunit vaccine and preparation method thereof

A technology of porcine pseudorabies virus and swine fever virus, applied in biochemical equipment and methods, vaccines, viruses, etc., can solve the problems of increased difficulty in the prevention and treatment of porcine pseudorabies, easy secondary diseases, immune system damage, etc., to reduce artificial Cost, simple preparation method, strong effect of antigen immunity

Active Publication Date: 2022-03-11
天康制药股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After pigs are infected with PRV, their own immune system is damaged and their immunity is reduced, so they are more likely to develop other diseases, such as swine fever, porcine reproductive and respiratory syndrome, etc., which makes it more difficult to prevent and treat porcine pseudorabies.

Method used

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  • Swine fever and porcine pseudorabies virus bivalent subunit vaccine and preparation method thereof
  • Swine fever and porcine pseudorabies virus bivalent subunit vaccine and preparation method thereof
  • Swine fever and porcine pseudorabies virus bivalent subunit vaccine and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2

[0043]Example 2 Determination of the Antigen Ratio of Classical Swine Fever and Porcine Pseudorabies Virus Double Subunit Vaccine

[0044] In order to determine the effective ratio of classical swine fever virus antigen and porcine pseudorabies virus antigen, the ratio was adjusted according to different antigen contents, mixed with ISA 201VG and emulsified, and 8 groups of vaccine samples were prepared for mouse immunization experiments in the laboratory.

[0045] 1. Vaccine preparation: After measuring the concentration of CSFV-E2, PRV-gB and PRV-gD proteins, according to the protein content in Table 1, emulsify with sterile 201 adjuvant (purchased from SEPPIC company) to prepare a double subunit vaccine, place Store at 4°C until use.

[0046] 2. For the determination of immunogenicity, 90 healthy KM mice, weighing about 20 g, were randomly divided into 9 groups. Groups 1 to 8 were immunized with vaccines with different antigen ratios, and 10 non-immunized mice were set as ...

Embodiment 3

[0051] The immunoprotective effect of embodiment 3 classical swine fever, porcine pseudorabies virus dual subunit vaccine

[0052] Thirty healthy susceptible pigs aged 3 to 4 weeks were used, grouped according to Table 3, and fed under the same conditions. Fourteen days after the first immunization, the second immunization was carried out in the same way and at the same dose, and serum neutralizing antibody was detected 14 days after the second immunization. 5 pigs in the immunization group and 5 pigs in the control group were intramuscularly injected with 1.0ml of CSFV Shimen blood virus (containing 10 5.0 The minimum lethal dose) was continuously observed for 16 days; 5 pigs in the immunization group and 5 pigs in the control group were inoculated with CJ strain of porcine pseudorabies virus (containing 2×10 5.5 TCID 50 ) 2.0ml, 1.0ml / nostril, observed continuously for 14 days.

[0053] Table 3 Test grouping

[0054]

[0055] Note: "N / A" (Not Available) means not appl...

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Abstract

The invention provides a swine fever and porcine pseudorabies virus bivalent subunit vaccine and a preparation method thereof. The effective components of the bivalent subunit vaccine are classical swine fever virus E2 protein, porcine pseudorabies virus gB protein and porcine pseudorabies virus gD protein. The preparation method of the bivalent subunit vaccine is simple, the swine fever virus E2 protein, the porcine pseudorabies virus gB protein and the porcine pseudorabies virus gD protein are high in yield, high in purity and strong in antigen immunity, the immune response of a body can be effectively activated, and the bivalent subunit vaccine has an ideal immune protection effect on swine fever and porcine pseudorabies. Compared with a monovalent vaccine, the artificial cost can be reduced, the number of vaccination times (double prevention by one injection) is reduced, the stress reaction of pigs caused by vaccine immunization is reduced, the protection effect is equivalent to that of the monovalent vaccine, and the antibody neutralizing result is superior to that of the monovalent vaccine.

Description

technical field [0001] The invention relates to the field of veterinary vaccines, in particular to a dual subunit vaccine of swine fever and porcine pseudorabies virus and a preparation method thereof. Background technique [0002] Classical swine fever (CSF) is an acute and highly contagious infectious disease caused by classical swine fever virus (CSFV). characteristics, the outbreak of CSF seriously restricts the healthy development of pig industry. [0003] Porcine pseudorabies (porcine pseudorabies, PR) is caused by pseudorabies virus (Pseudorabiesb virus, PRV), one of the important infectious diseases that seriously endanger the healthy development of my country's pig industry. The virus belongs to the linear double-stranded DNA virus of the herpesviridae herpesvirus A subfamily. At present, there are 11 kinds of viral glycoproteins, among which gG is a secreted protein, and the remaining 10 kinds of glycoproteins are all anchored in the viral envelope. It functions ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/18C07K14/03A61K39/12A61K39/245A61K39/39A61P31/14A61P31/22
CPCC07K14/005A61K39/12A61K39/39A61K31/14A61K31/22C12N2770/24322C12N2770/24334C12N2710/16722C12N2710/16734A61K2039/552A61K2039/70
Inventor 贺笋师小潇候凤李俊辉王遵宝李晓梅张超
Owner 天康制药股份有限公司
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