Pseudosciaena crocea interferon (IFN) gene and cloning method thereof

A large yellow croaker and interferon technology, applied in the field of molecular biology, can solve the problems of virus secondary infection, fish death, information reporting of large yellow croaker interferon gene sequence, etc., and achieves the effect of wide application value and environmental friendliness

Inactive Publication Date: 2012-07-11
JIMEI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, at present, the cultivation of large yellow croaker is seriously threatened by various diseases, among which the secondary infection of viruses is one of the most important causes of large-scale fish kills
However, there is no report on the sequence information of the large yellow croaker interferon gene

Method used

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  • Pseudosciaena crocea interferon (IFN) gene and cloning method thereof
  • Pseudosciaena crocea interferon (IFN) gene and cloning method thereof
  • Pseudosciaena crocea interferon (IFN) gene and cloning method thereof

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Embodiment 1

[0016] A cloned large yellow croaker interferon gene in this embodiment has the base sequence of SEQ ID NO.1 in the sequence listing.

[0017] (1) Information for SEQ ID NO 1 (see sequence listing)

[0018] (a) Sequential features:

[0019] * Length: 878 base pairs

[0020] * Type: nucleic acid

[0021] * Chain type: double chain

[0022] * Topology: Linear

[0023] (b) Molecule type: cDNA

[0024] (c) Assumption: No

[0025] (d) Antisense: No

[0026] (e) Original source: Large yellow croaker ( Larimichthys crocea ).

[0027] A cloned large yellow croaker interferon gene of this embodiment has the amino acid sequence shown in SEQ ID NO.2 in the sequence listing:

[0028] (2) Information of SEQ ID NO.2 (see sequence listing)

[0029] (a) Sequential features

[0030] * Length: 185 amino acids

[0031] * Type: amino acid

[0032] * Chain type: single chain

[0033] * Topology: Linear

[0034] (b) Molecule Type: Protein

[0035] Wherein the cloning method of the...

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Abstract

The present invention discloses a Pseudosciaena crocea interferon gene, which is characteristics in that: the cDNA (complementary DNA) sequence of the coding region of the interferon protein has the base sequence shown in SEQ ID NO.1 and the amino acid sequence shows in SEQ ID NO.2 in the sequence list; as well as a cloning method thereof. The Pseudosciaena crocea interferon gene disclosed by the invention has a good application prospect in prevention and control of Pseudosciaena crocea disease and further development of biological fish drugs, and lays the foundation for Pseudosciaena crocea molecular marker-auxiliary disease resistance breeding and detection of health status of fishes.

Description

technical field [0001] The invention relates to a full-length cDNA sequence of large yellow croaker interferon (IFN) gene and a cloning method thereof, belonging to the technical field of molecular biology. Background technique [0002] Large yellow croaker is the fish with the largest amount of seawater cage culture in my country, with an annual output of 70,000 tons and an annual output value of more than 3 billion yuan. However, at present, the cultivation of large yellow croaker is seriously threatened by various diseases, among which the secondary infection of viruses is one of the most important causes of large-scale fish mortality. [0003] The role of interferon was confirmed in 1957 [Isaacs A, Lindenmann J, 1957. Virus interference. I. The interferon. Proc R Soc Lond B Biol Sci. 147: 258-267]. But it was not until 2003 that the first fish interferon gene was cloned from zebrafish [Altmann SM, Mellon MT, Distel DL, Kim CH, 2003. Molecular and functional analysis of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/20C12N15/10
Inventor 姚翠鸾李婵王志勇
Owner JIMEI UNIV
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