Method for preserving microbial strains for long time
A technology of microbial strains and culture medium, which is applied in the field of microbial preservation, can solve the problems of resource gain and loss, industrial production loss, degradation and variation, etc., and achieve the effects of reducing the probability of variation, reducing energy consumption, and making production simple
Inactive Publication Date: 2012-04-04
SHANGHAI HAIDI GARDENING
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Problems solved by technology
However, acetic acid bacteria is different from other bacterial species, it is very easy to mutate, and regular slant passage increases the probability of degradation and variation, which will make the precious bacteria resources obtained by optimization lost again, and even cause great harm to industrial production. great loss and harm
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Embodiment 1
[0028] Inoculate the As 1.41 strain on the slant medium, cultivate at 30°C for 24h, add a little calcium carbonate that has been sterilized by dry heat at 200°C for 2h on the slant, and pour the liquid test tube medium into the test tube to a height of 2cm. , replace with a sterilized rubber stopper. Store in refrigerator at 2-4°C. After being preserved for 3, 6, 9, 12, 15, 18, 21, and 24 months, they were taken out for observation. After a few days, the acetic acid bacteria preserved by the preservation method were inoculated into the proliferation triangular flask, and cultured at 30 "C for 48 hours. The % inoculum was transferred to the triangular flask for acid production, cultured at 30°C, and the acidity was measured every day. The fermentation experiment was terminated after 7 days.
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Abstract
The invention relates to a microbial strain preserving method, in particular to a method for preserving microbial strains for a long time. The method comprises the main steps of: inoculating the strains onto a slant culture medium; cultivating the strains for 20-30 hours at a temperature of 20-40 DEG C; adding a little calcium carbonate onto a slope, wherein the calcium carbonate is subjected to dry heat sterilization; filling a liquid tubular culture medium into a test tube; plugging the test tube by using a sterile rubber plug; and putting the test tube in a refrigerator for preservation at a temperature of 2-4 DEG C. When the method is adopted to preserve acetic acid strains, reproducing times of the acetic acid strains can be reduced, namely mutation probability can be reduced. The method is simple to operation; the preservation period can reach 2 years; and the microbial strains can be put into production after being activated by using the slant culture medium for two times when used, without complex operation processes. In addition, the purpose of preservation can be achieved without any expensive instrument; the method is suitable for mass production; and according to the method, cost can be lowered, and energy consumption can be reduced.
Description
technical field [0001] The present invention relates to a method for preserving microorganisms, in particular to a method for long-term preservation of microorganisms. Background technique [0002] Bacteria is an important resource of the country, and it is the basic material for microbiology and life science research, especially the use of microorganisms for related production is inseparable from bacteria. Therefore, the preservation of strains is an important part of microbiological research and microbial breeding, and its task is to adopt the most appropriate preservation methods to minimize the variation and death of strains. [0003] The conventional preservation methods of acetic acid bacteria in microbial strains are slant preservation and liquid paraffin preservation, which require regular passage. But Acetobacter is different from other strains in that it is very easy to mutate, and regular slant passaging increases the probability of degradation and variation, whi...
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IPC IPC(8): C12N1/04C12R1/02
Inventor 丁国才
Owner SHANGHAI HAIDI GARDENING
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