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Method for preparing spiramycin base producing strain gray streptomyces ambofaciens spores

A technology of spiramycin base and Streptomyces griseus, which is applied in the biological field, can solve the problems of decreased production capacity of strains, short storage period, and increased passage times, etc., so as to prevent strain degradation, prolong storage time, and improve work efficiency. Effect

Inactive Publication Date: 2015-11-18
TOPFOND PHARMA CO LTD
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Problems solved by technology

As we all know, the continuous repeated cultivation process will lead to a decrease in the production capacity of the strains, while mycelia are frozen and stored, and the storage period is short, and the batch numbers of the strains have to be frequently changed during production, which increases the number of passages, resulting in unstable production. Increased the workload of strain preparation

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  • Method for preparing spiramycin base producing strain gray streptomyces ambofaciens spores
  • Method for preparing spiramycin base producing strain gray streptomyces ambofaciens spores

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Embodiment 1

[0021] A method for preparing spiramycin base to produce bacteriocin Streptomyces griseus spores, comprising the following steps:

[0022] a. Preparation of plate medium:

[0023] (1) Prepare the plate according to the ratio of 4% corn starch, 0.03% amylase, 1% soybean ice powder, 0.4% sodium chloride, 0.05% magnesium sulfate heptahydrate, 0.5% calcium carbonate, 1% agar, and distilled water as the balance Culture medium;

[0024] (2) Put the plate medium above in a water bath at 85°C for 15 minutes, then distribute it into 500ml Erlenmeyer flasks while shaking well, each bottle contains 200ml; sterilize at 0.1MPa and 121°C for 30 minutes, Keep the pressure and cool down after the bacteria are finished;

[0025] (3) Shake the sterilized plate separation medium while it is hot, and pour it into sterilized petri dishes with a diameter of 90mm while cooling to about 60°C, with about 25ml of plate culture medium in each dish; Put the well-inverted culture dish flat and cover i...

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Abstract

The invention belongs to the technical field of biology, and particularly relates to a method for preparing spiramycin base producing strain gray streptomyces ambofaciens spores. According to the method, a plating medium is prepped from 4% of corn starch, 0.03% of amylase, 1% of soybean cake meal, 0.4% of sodium chloride, 0.05% of magnesium sulfate heptahydrate, 0.5% of calcium carbonate, 1% of agar and the balance of distilled water according to a ratio; and the plating medium is sterilized and then poured into a culture dish. By adopting the novel preparation method provided by the invention, the spores can be produced, and spore slants can be prepared into a milk tube for culture preservation and can be stored for 6 years at least.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for preparing spores of the spiramycin base-producing bacteria Streptomyces griseus. Background technique [0002] Spore preparation is an indispensable link in fermentation industrial production, and the quality of spores will directly affect the success of fermentation production. The Streptomyces griseus used in domestic production of spiramycin alkaline fermentation is obtained through mutagenesis, and generally no spores can be obtained. The synthetic gene part of spiramycin SPM is a plasmid gene, and the plasmid gene is easily lost. Therefore, the spiramycin SPM production strain is not very stable, and its performance is easy to decline. The maintenance of strain production capacity is the most important issue in strain breeding and antibiotic production. As we all know, the continuous repeated cultivation process will lead to a decrease in the prod...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N3/00C12R1/545
Inventor 刘守强冯汇朱晓莉赵抗洪刘俭国翟明礼孟新梅刘丽杰王雪洁薛娟陈双王蕊周晨曦李慧
Owner TOPFOND PHARMA CO LTD
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