Simple method for preserving rhizopus oryzae strains for brewing fermented glutinous rice for long time

Abstract

The invention discloses a simple method for preserving rhizopus oryzae strains for brewing fermented glutinous rice for a long time. The method is characterized in that cob, oatmeal. Millet and quinoa are used as the raw materials, then procedures such as preservation culture medium preparation, inoculation, drying and preservation are performed to obtain the rhizopus oryzae strains for brewing fermented glutinous rice, and the rhizopus oryzae strains can be preserved for 8 years. The method has the advantages that the method is simple to operate, high in feasibility, safe and reliable, low in equipment requirement, long in preservation time and large in preservation amount, expensive equipment such as an ultralow-temperature refrigerator, a freeze drying device and a liquid nitrogen preservation device is not needed, the strains are stable in production performance, the good properties of the strains do not degenerate, the strains do not mutate and are high in survival rate, frequent passage is not needed, passage times are reduced to the maximum extent, high inoculation efficiency is achieved, strain mutation and mixing caused by to too much passage can be prevented effectively, a preservation matrix is cheap, high in quality and wide in source, and the preserved strains can be taken and used at any time.

Description

technical field [0001] The invention relates to a strain preservation method, in particular to a simple method for long-term preservation of Rhizopus oryzae strains for brewing fermented glutinous rice. Background technique [0002] Fermented glutinous rice is one of the popular traditional Chinese folk fermented foods. It is called glutinous rice wine in the north; fermented glutinous rice wine, glutinous rice wine, etc. in the Central Plains; Fermented glutinous rice is made by saccharifying and fermenting glutinous rice with Rhizopus oryzae. It has the aroma of wine and pure rice aroma. It is rich in nutrition and contains oligosaccharides, amino acids, polypeptides, various trace elements and vitamins C, A, B, D, E, K Etc. Appropriate regular drinking can promote blood circulation, improve immunity, promote metabolism, and also have the effects of nourishing blood and beautifying skin, relaxing tendons and activating blood circulation, and prolonging life. This fermented...

AI Technical Summary

Problems solved by technology

The characteristic of this preservation technique is to continuously transplant the strains on fresh medium, culture them under suitable conditions, and then place them in a refrigerator at 4-10°C. This preservation technique mainly includes slant transplantation, tubing preservation and distilled water preservation The advantages of the slope cryopreservation method are simple operation, convenient use, and no need for special equipment, but due to the need for frequent subculture, it is easy to mutate and degrade, and there are more chances of contaminating bacteria

[0005] The second is to preserve the dormant body of the bacterial strain in a vacuumized and dry container, such as cystspores, conidia, chlamydospores, etc. The drying matrix can be silica gel, calcium oxide, etc. This preservation technology can preserve strains 1- 2 years, but vacuum facilities are needed, the desiccant needs to be changed and vacuum pumped 1-2 times during the storage process, and the operation is relatively cumbersome

[0010] 1. Shen Fayi introduced the long-term preservation method of rhizopus in the article "Preservation and Rejuvenation of Brewing Aspergillus" in the 10th issue of "Winemaking Science and Technology" in 2009. method (15-18 years), liquid nitrogen tank ultra-low temperature preservation method (10 years), although the latter two methods have a long storage time, they have high equipment requirements, large equipment investment, complicated methods, and high operation requirements. I can't do it, and I don't have these facilities

[0011] 2. Gu Jingang, Li Shigui, etc. introduced in the article "Research Progress in Fungal Preservation Technology" in "Journal of Mycology", Vol. The development of this method is bound to be related to safe and long-term preservation technology methods. For strains with important economic value, the damage and variation of physiological and genetic traits in the process of preservation technology and preservation will bring huge losses.

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