Mixtures of ginsenosides and their use as bidirectional telomere modulators

A technology of ginsenosides and mixtures, applied in plant growth regulators, plant growth regulators, preservation of human or animal bodies, etc., can solve problems such as few researches, reduce the probability of cell mutation, and shorten the lifespan of plant cell lines , the effect of prolonging the lifespan of plant cell lines

Active Publication Date: 2019-03-22
INST OF CHINESE MATERIA MEDICA CHINA ACAD OF CHINESE MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Previous studies have reported on the relationship between telomere length and tree age in woody plants such as ginkgo, bristlecone pine, and Chinese pine, but there are few studies on the maintenance of plant telomere length, especially whether the active ingredients of traditional Chinese medicine have an effect on telomere length maintenance rarely reported

Method used

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  • Mixtures of ginsenosides and their use as bidirectional telomere modulators
  • Mixtures of ginsenosides and their use as bidirectional telomere modulators
  • Mixtures of ginsenosides and their use as bidirectional telomere modulators

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0088] Embodiment 1, the preparation of ginsenoside mixture

[0089] One, the preparation of ginsenoside mixture A

[0090] 1. Mix ginsenoside Rg1, ginsenoside Re and ginsenoside Rh2 in a mass ratio of 3:3:1 to obtain ginsenoside mixture A1.

[0091] 2. Mix ginsenoside Rg1, ginsenoside Re and ginsenoside Rh2 according to the mass ratio of 2:1:3 to obtain ginsenoside mixture A2.

[0092] Two, the preparation of ginsenoside mixture B

[0093] 1. Mix ginsenoside Rb1, ginsenoside Rd, and ginsenoside Rb3 in a mass ratio of 1:2:2 to obtain ginsenoside mixture B1.

[0094] 2. Mix ginsenoside Rb1, ginsenoside Rd, and ginsenoside Rb3 in a mass ratio of 3:3:1 to obtain ginsenoside mixture B2.

Embodiment 2

[0095] Example 2, Application of Ginsenoside Mixture A in Prolonging Telomere Length of Ginseng Suspension Cells

[0096] 1. Ginseng Suspension Cell Culture

[0097] 1. Inoculate the main root of ginseng on the callus induction medium (MS+2.0mg L -1 NAA+1.0mg·L -1 6-BA) in (26±1)°C under dark conditions for 30 days, and after subcultured for 3 times, callus with vigorous growth and loose texture was selected.

[0098] 2. Inoculate the callus obtained in step 1 into a liquid medium (MS+0.5mg L -1 KT+1.0mg·L -1 2,4-D), speed 100r min -1 , (26±1)°C, and cultured in the dark for 15 days to obtain ginseng suspension cells with a uniform and stable system and good cell viability.

[0099] 2. Treatment of ginseng suspension cells with ginsenoside mixture A

[0100] Experimental group I: Add the ginsenoside mixture A1 prepared in Example 1 to the 100 mL ginseng suspension cell culture medium subcultured in step 1 for one week, and the concentration of the ginsenoside mixture A1 ...

Embodiment 3

[0123] Example 3, Application of Ginsenoside Mixture B in Shortening Ginseng Suspension Cell Telomere Length

[0124] 1. Treatment of ginseng suspension cells with ginsenoside mixture B

[0125] Experimental group I: The ginsenoside mixture B1 prepared in Example 1 was added to the ginseng suspension cells subcultured for one week in step 1 of Example 2, and the concentration of the ginsenoside mixture B1 in the culture system was 5 μg / mL.

[0126] Experimental group II: The ginsenoside mixture B1 prepared in Example 1 was added to the ginseng suspension cells subcultured for one week in step 1 of Example 2, and the concentration of the ginsenoside mixture B1 in the culture system was 50 μg / mL.

[0127] Experimental group III: Add the ginsenoside mixture B1 prepared in Example 1 to the ginseng suspension cells subcultured for one week in step 1 of Example 2. The concentration of the ginsenoside mixture B1 in the culture system is 200 μg / mL.

[0128] Experimental group IV: Add t...

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Abstract

The invention discloses ginsenoside mixtures and application of the ginsenoside mixtures serving as bidirectional telomere modifiers. The ginsenoside mixture B is composed of ginsenoside Rb1, ginsenoside Rd and ginsenoside Rb3, and the mass ratio of the ginsenoside Rb1, the ginsenoside Rd to the ginsenoside Rb3 is 1-3: 2-3: 1-2. The ginsenoside mixture A is composed of ginsenoside Rg1, ginsenoside Re and ginsenoside Rh2, and the mass ratio of the ginsenoside Rg1, the ginsenoside Re to the ginsenoside Rh2 is 2-3: 1-3: 1-3. The ginsenoside mixture A serves as the telomere prolonging agent, the ginsenoside mixture B serves as the telomere shortening agent, and the ginsenoside mixture A and the ginsenoside mixture B are used jointly so that the plant telomere length can be effectively maintained and the plant life regulation and control can be studied. The ginsenoside mixtures and the application of the ginsenoside mixtures serving as the bidirectional telomere modifiers have important significance for development of novel telomere modifiers and study of the plant life regulation and control.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a ginsenoside mixture and its application as a two-way telomere regulator. Background technique [0002] In the process of plant growth and development, the higher the differentiation degree of tissues and organs, the shorter the telomere length. At the same time, the length of the DNA sequence in the telomere is also a molecular marker of plant aging and lifespan. Therefore, the maintenance of telomere length is of great significance for plant longevity. . Telomere DNA sequences are non-structural genes and do not have the function of coding proteins. Telomeres are located at the ends of linear chromosomes in eukaryotic organisms, similar to a special structure like a "cap", which is of great significance for maintaining the integrity and stability of chromosomes and controlling the cell division cycle. [0003] Telomeres are mainly composed of telomere DNA and telomere-related pr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N43/16A01P21/00A01G7/06
CPCA01G7/06A01N43/16A01N2300/00
Inventor 黄璐琦袁媛纪瑞锋刘娟张迎春陈同
Owner INST OF CHINESE MATERIA MEDICA CHINA ACAD OF CHINESE MEDICAL SCI
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