Tissue culture method of Hosta plantiginea Ascherson

A tissue culture and white hosta technology, applied in the field of plant tissue culture, can solve problems such as no report on the white flower hosta tissue culture technology, and achieve the effects of reducing the probability of variation, simple operation and high reproduction coefficient

Inactive Publication Date: 2011-04-06
沈阳市农业科学院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are only a small amount of reports on the germplasm resources of the genus Hosta and garden applications, tissue culture of ornamental hosta species in various domestic literatures, but there is no report on the tissue culture technology of the white flower hosta

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] 1. In spring, cut the new buds with a length of about 1 cm in the current year, rinse with washing powder water for 5 minutes, rinse with running water for 30 minutes, treat with 70% alcohol on the ultra-clean workbench for 10 seconds, and then use 0.1% mercury chloride Soak and shake for 6 minutes, wash 6 times with sterile water, absorb water with filter paper, and insert into induction medium: MS+BA 0.2mg / L +NAA 0.01mg / L , additional agar powder 0.6%, sucrose 3%. The culture temperature was 20 °C, the light intensity was 2000 Lux, and the light was 14 hours a day. After 35 days, sterile seedlings were basically formed. The pollution rate is 28%, and the seedling rate is 100%.

[0020] 2. Peel off the outer leaves of the test tube seedlings and inoculate them in MS+BA 2mg / L +NAA 0.1mg / L On the medium of 15 days or so, new axillary buds began to sprout from the exposed leaf scars. After 35 days, most of the axillary buds are more than 1 cm long, and they can be d...

Embodiment 2

[0023] The process of Example 1 was repeated, and the induction medium was supplemented with the hormones BA 0.5 mg / L and NAA 0.05 mg / L. The seedling rate is 100%.

[0024] Proliferation medium is MS+BA 3mg / L +NAA 0.3mg / L , bud differentiation number 3.67.

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PUM

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Abstract

The invention relates to a method for cultivating jade needle organism, wherein it comprises that: using fresh bud, via initial cultivation to form germ-free seed, without cure organism to induce the bud to increment, to root and cultivate. The invention has simple process and high yield.

Description

technical field [0001] The invention relates to a method for culturing hosta flower tissue and belongs to the technical field of plant tissue culturing. Background technique [0002] Hosta flowers are native to the East and are widely used around the world as garden ornamental and medicinal plants. Hosta plantiginea Ascherson native to my country, also known as Yuchun stick, white crane fairy, and white calyx, is the only type of hosta with white flowers and strong aroma. The whole plant is slightly poisonous, and the flowers have the effect of clearing away heat and moistening the lungs. However, white hosta is propagated by division method in nature, and the growth and reproduction speed is very slow, which restricts its wide application. If it can be propagated by tissue culture, it is expected to produce a large number of test-tube seedlings with the same characters in a short time. [0003] At present, there have been systematic and in-depth studies on the classifica...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00C12N5/04
Inventor 赵江雷潘菊张同臣卢文经马文宏张健朴凯伟甄广田张曼丽
Owner 沈阳市农业科学院
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