Induction method of callus of sterile seedling prepared from root bud of Amomum villosum Lour.
A callus and rhizome technology is applied in the field of callus induction, which can solve the problems of limited use time, low reproduction coefficient and the like, and achieve the effects of stable medicinal properties, abundant sources and not easy to mutate.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0035] A method for inducing callus of aseptic seedlings prepared from the rhizome buds of P. chinensis, comprising the following steps: cutting the stem of the aseptic seedling of P. chinensis into 2cm segments, and aseptically operating and sterilizing the stem segments Finally, vertically place the inoculation in the prepared and sterilized medium for cultivation, inoculate 5 bottles, 1 block per bottle, observe and record every 3 days. The well-growing callus was selected by culture, and the successfully induced callus was subcultured once every 30 days.
[0036] The environmental conditions for cultivation are as follows: temperature: 22°C; relative air humidity: 50%; light control is 10 hours of light, and light intensity is 1500Lx.
[0037] The medium formula is: MS+1.0mg / L 6-BA+0.5mg / L 2,4-D+0.5mg / L NAA
Embodiment 2
[0039] The steps of the method for inducing the callus of the aseptic seedlings prepared from the rhizome buds of Yangchunsha in this example are the same as those in Example 1.
[0040] The cultivation environment conditions in this embodiment are as follows: temperature: 25°C; relative air humidity: 80%; light control is 15 hours of light, and the light intensity is 2000Lx.
[0041] The medium formula is: MS+2.5mg / L 6-BA+1.5mg / L 2,4-D+1.5mg / L NAA
Embodiment 3
[0043] The steps of the method for inducing the callus of the aseptic seedlings prepared from the rhizome buds of Yangchunsha in this example are the same as those in Example 1.
[0044] The cultivation environment conditions in this embodiment are as follows: temperature: 25°C; relative air humidity: 80%; light control is 10 hours of light, and light intensity is 2000Lx.
[0045] The medium formula is: MS+1.5mg / L 6-BA+1.0mg / L 2,4-D+0.5mg / L NAA
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com