Method for tissue-culture breeding and wild-simulated cultivation of dendrobium candidum

Abstract

The invention discloses a method for tissue-culture breeding and wild-simulated cultivation of dendrobium candidum, which comprises: inoculating dendrobium candidum tender straw slices onto a culturemedium for culture under conditions of a temperature of 22 to 24 DEG C and an illuminance of 1,000 to 1,500 Lx to first induce the growth of calles to form protocorms and then promote the multiplicative growth of the protocorms into 5 to 8 centimeter strong seedlings; and moving the seedlings out of a culture room, hardening the seedlings at a normal temperature for 3 to 5 days and transplanting the seedlings into the ground in a green house. The plane basic layer of a cultivation check field in the green house are provided with spaced breather holes; the upper layer of the cultivation check field is a cultivation medium; the seedlings are planted at a row spacing of 12*8cm and a spacing in the row of 12*8cm; in the green house, the temperature is 0 to 28 DEG C, the humidity is 60 to 70 percent and the shade density is 70 to 80 percent; and disease and pest prevention and control are carried out periodically and the dendrobium candidum can be harvested in 3 to 4 years. The method improves the civil year breeding coefficient of the dendrobium candidum by about 10 times, provides enough seedlings for large-scale cultivation, solves the problem of wild-simulated cultivation and realizes scale cultivation.

Description

technical field [0001] The invention belongs to the field of plant regeneration through tissue culture technology, and specifically relates to a dendrobium candidum tissue culture and a wild-imitation cultivation method. Background technique [0002] Dendrobium is a plant of the genus Dendrobium in the family Orchidaceae. The main medicinal species of Dendrobium are Dendrobium annua, Dendrobium versicolor, Dendrobium yellow grass, Dendrobium candidum and Dendrobium nobile. Especially Dendrobium officinale and Dendrobium nobile have the highest medicinal value. Medicinal Dendrobium is mainly used for nourishing yin and clearing away heat, and can treat acute and chronic pharyngitis, acute bronchitis, tuberculosis, hyperthyroidism, diabetes, chronic Atrophic gastritis and low immune function, etc., and has health effects of enhancing body immunity and anti-aging for middle-aged and elderly people. [0003] Dendrobium officinale has a special natural wild environment. It grows...

AI Technical Summary

Problems solved by technology

In recent years, people want to propagate Dendrobium officinale through artificial cultivation. Although some progress has been made, it has not been able to solve the problems of low survival rate, high production cost and inability to produce on a large scale.

Chinese patent application number 200410017380.6 discloses "a kind of cultivation method of Dendrobium officinale", and this technology adopts 2000~3000lx light on the light intensity of tissue culture seedlings, and its advantage is that can shorten light time and tissue culture seedling culture period, but there is leaf factor Violet red occurs when the light intensity is high, and the chlorophyll content is low, which is not conducive to the robust growth of tissue culture seedlings; Chinese patent application number 200410084496.1 also discloses a "artificial large-area cultivation method for Dendrobium officinale", which uses The seeds of Dendrobium officinale are propagated by tissue culture, and then the tissue culture seedlings are transplanted in two stages. First, after 4 to 5 months of hardening and transplanting, the hardened Dendrobium seedlings are transplanted to the production field

The advantage of this method is that the amount of seed propagation is large, it is easy to form protocorms, and it can improve the survival rate of transplanting. Its disadvantages are that the seed propagation is prone to character separation, the population character is unstable, the production cost is high, and the workload is large, which is not conducive to large-scale production. Production