Artificial cultivation and seedling industrial production method of dendrobium nobile polyploid compound

A production method, the technology of Dendrobium orchid, is applied in the cultivation of artificial polyploid complex of spring Dendrobium orchid and the industrial production of seedlings, which can solve the problems of narrow range of hybridization variation and low frequency of new variety selection, and achieve disease resistance The effect of strong sex and vigorous growth

Active Publication Date: 2011-11-16
NANKAI UNIV
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Problems solved by technology

These methods have the problems of narrow range of hybridization va

Method used

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  • Artificial cultivation and seedling industrial production method of dendrobium nobile polyploid compound
  • Artificial cultivation and seedling industrial production method of dendrobium nobile polyploid compound
  • Artificial cultivation and seedling industrial production method of dendrobium nobile polyploid compound

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Embodiment Construction

[0033] The cultivation of a kind of dendrobium orchid artificial polyploid complex provided by the invention and its seedling industrial production method comprise the following steps:

[0034]Carry out artificial pollination at the flowering stage of common diploid Dendrobium orchid, take the harvested capsules, inoculate the seeds in 1 / 2MS liquid medium for non-symbiotic germination culture, and move the protocorm of the proembryo seed into a seed containing 0.01% colchicine 1 / 2MS liquid medium for mutagenesis culture, and then transferred to 1 / 2MS solid medium for light culture until the spring Dendrobium orchid seedlings, the tetraploid seedlings with chromosome number 2n=76 were selected for flowering culture, after flowering, Take tetraploid as female parent and diploid as male parent (4x♀×2x♂) or diploid as female parent and tetraploid as male parent (2x♀×4x♂) to obtain triploid spring Dendrobium. Triploid Dendrobium hybrid seed formation early stage (protoembryo stage...

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Abstract

The invention relates to an artificial cultivation and seedling industrial production method of a dendrobium nobile polyploid compound, which comprises the following steps of: carrying out artificial pollination in a flowering period of common dendrobium nobile, inoculating harvested seeds in a 1/2MS liquid nutrient medium for nonsymbiotic germination cultivation, transferring protocorm of seeds in a proembryo stage into a 1/2MS liquid nutrient medium containing 0.01% of colchicin for mutagenesis cultivation, then transferring into 1/2MS solid nutrient medium for cultivation till seedlings ofdendrobium nobile are formed, carrying out flowering cultivation on tetraplont seedlings with chromosome numbers of 2n=76, taking tetraplont as a female parent and diploid as a male parent or taking diploid as a female parent and tetraplont as a male parent for hybridization to obtain the triploid; transferring triploid hybridized seeds in a formation early state into the 1/2MS liquid nutrient medium containing 0.01% of colchicin for carrying out artificial mutation hexaploid treatment for 150-180h, and then transferring into the 1/2MS solid nutrient medium till the seedlings are formed, selecting seedlings with chromosome numbers of 2n=114 as tetraplont dendrobium nobile for carrying out bottle seedling transplanting, and finally cultivating and managing according to a conventional method till flowering. The invention lays the foundation for the popularization of new varieties of the dendrobium nobile.

Description

technical field [0001] The invention relates to a method for artificially cultivating orchids and an industrialized production technology for seedlings thereof, in particular to a method for cultivating artificial polyploid complexes of spring dendrobium orchid (Dendrobium Nobile) and an industrialized production method for seedlings. Background technique [0002] Dendrobium is one of the four largest ornamental orchids in the world. At present, a large number of ornamental varieties of Dendrobium orchids have been cultivated internationally. At the World Orchid Conference held in Tokyo in 2010, a Dendrobium orchid with more than 2,000 flowers won the gold medal. The cultivation of new varieties of Dendrobium orchids has become a new hot spot in flower breeding in the world. . However, so far, the cultivation of new species of Dendrobium orchids in the world has mostly been obtained by hybridization between species. For example, CN200710160571.1 and CN200710160572.6 disclo...

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Application Information

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IPC IPC(8): A01H4/00
Inventor 李秀兰陈力陈瑞阳
Owner NANKAI UNIV
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