Subfamily distant hybridization for German mirror carp and megalobrama amblycephala and application of tetraploid hybrid fishes

Abstract

The invention discloses a subfamily distant hybridization for German mirror carp and megalobrama amblycephala. The method comprises the following steps of artificial dry-process fertilization on the ova of the pubescent German mirror carp and sperms of the megalobrama amblycephala by taking the German mirror carp as a female parent and the megalobrama amblycephala as a male parent, hatching fertilized ova at the water temperature of 19-20 DEG C, and breeding fries in a pond; then, detecting the DNA (deoxyribonucleic acid) content, the chromosome number and the blood cell sizes of the fries by a flow cytometry DNA content determination method, a chromosome ploidy detection method of peripheral blood cell culture and a blood smear method, screening, and then preparing to obtain triploid hybrid fishes and tetraploid hybrid fishes. A tetraploid fish strain which is genetically stable and has four sets of crucian carp chromosomes can be formed by subsequent self-cross selective breeding of the tetraploid hybrid fishes, and the tetraploid fish strain can be used as a resource for breeding the infertile triploid fishes. The method disclosed by the invention has the advantages that not only are the characters of hybrid offspring good but also a good foundation can be laid for variety breeding, and a precious resource is provided for subsequent breeding of new polyploid fishes.

Description

technical field [0001] The invention relates to a fish hybridization method, in particular to a method for distant hybridization between subfamilies of crucian carp and bream. Background technique [0002] Hybridization technology is a common method for improving fish traits. It is a breeding method that integrates some of the excellent traits of two or more species through hybridization. It is one of the most basic fish breeding methods and is also a widely used breeding method. . Hybridization between species or above is generally called distant hybridization. As an important method of fish genetic breeding and trait improvement, distant hybridization can integrate a whole set of exogenous genes, thereby changing the expression regulation of hybrid progeny genes, so that hybrid progeny may improve in growth speed, stress resistance, disease resistance and Meat quality and other aspects showed heterosis. For example, the hybrid offspring formed by the intergeneric distan...

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Problems solved by technology

Although the previous hybridization of Japanese white crucian carp and bream was also carried out, only triploid hybrid offspring were found in the offspring, and no tetraploid was detected.

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