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Method for improving hybridization fertilization rate of female fugu obscurus and male fugu rubripes

A technology of red-finned pufferfish and dark-striped pufferfish, which can be used in fish farming, application, climate change adaptation, etc., and can solve problems such as difficulty in fertilization

Inactive Publication Date: 2019-04-12
JIANGSU ZHONGYANG GRP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the different osmotic pressures of the eggs and sperm of the two fishes, fertilization is more difficult. Whether the eggs developed in fresh water can be combined with the sperm developed in seawater and life can be cultivated depends on how to find the fertilization, embryo development , sperm motility, optimal concentration of saline for fertilization, and effective fertilization timeliness. At present, there is no report on the method of improving the cross fertilization rate of puffer puffer obscurus female parent and red fin puffer puffer male parent
[0005] Physiological saline refers to a sodium chloride solution whose osmotic pressure is basically equal to that of animal or human plasma in physiological experiments or clinical practice. It is 0.67~0.70% for amphibians and 0.85% for mammals and humans. ~0.9%, the concentration of normal saline for freshwater and seawater fish hybridization has not been reported, therefore, an improved technology is urgently needed to solve this problem existing in the prior art

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A method for improving the hybrid fertilization rate of puffer puffer females and puffer pufferfish males, comprising the following steps:

[0030] Step 1: Parental selection: select the 3-year-old, 800g in body weight, healthy, healthy, disease-free and non-injured female parent of Fugu obscura with mature gonads and the 3-year-old, 3200g in weight, healthy body, no disease and injury, and mature gonads. The male parent of the redfin puffer is used as the breeding parent;

[0031] Step 2: Parent male and female identification and sex ratio: the female parent’s abdomen is enlarged, the genital opening is protruding, slightly chrysanthemum-shaped, congested, the gonads develop to about IV stage, no eggs are discharged by light pressure on the abdomen, the gonads develop to V stage, light pressure There are beige eggs discharged from the abdomen; the male parent has a small abdomen, lightly presses the genital opening on the abdomen, and has an inverted funnel-shaped repr...

Embodiment 2

[0036] A method for improving the hybrid fertilization rate of puffer puffer females and puffer pufferfish males, comprising the following steps:

[0037] Step 1: Parental selection: Select 3-year-old Fugu obscura female parent, weighing more than 850g, healthy, disease-free, and mature gonads, and 3-year-old, weighing more than 3300g, healthy, healthy, disease-free, and gonad-developed Mature redfin puffer male parent as breeding parent;

[0038] Step 2: Parent male and female identification and sex ratio: the female parent’s abdomen is enlarged, the genital opening is protruding, slightly chrysanthemum-shaped, congested, the gonads develop to about IV stage, no eggs are discharged by light pressure on the abdomen, the gonads develop to V stage, light pressure There are beige eggs discharged from the abdomen; the male parent has a small abdomen, lightly presses the genital opening on the abdomen, and has an inverted funnel-shaped reproductive process. When the gonad developme...

Embodiment 3

[0043] A method for improving the hybrid fertilization rate of puffer puffer females and puffer pufferfish males, comprising the following steps:

[0044] Step 1: Parental selection: Select 4-year-old pufferfish obscurus, weighing more than 900g, healthy, healthy, disease-free, and mature gonads, and 4-year-old, weighing more than 3,500g, healthy, healthy, disease-free, and gonad-developed Mature redfin puffer male parent as breeding parent;

[0045] Step 2: Parent male and female identification and sex ratio: the female parent’s abdomen is enlarged, the genital opening is protruding, slightly chrysanthemum-shaped, congested, the gonads develop to about IV stage, no eggs are discharged by light pressure on the abdomen, the gonads develop to V stage, light pressure There are beige eggs discharged from the abdomen; the male parent has a small abdomen, lightly presses the genital opening on the abdomen, and has an inverted funnel-shaped reproductive process. When the gonad develo...

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Abstract

The invention discloses a method for improving the hybridization fertilization rate of female fugu obscurus and male fugu rubripes. According to the method, a fugu obscurus female parent and a fugu rubripe male parent, which are large in size, healthy and sexually mature, are mainly adopted as breeding parents, sperms and ova are thoroughly mixed by a dry method after once pre-ripening physiological regulation, and the sperms and the ova are combined by adjusting osmotic pressure, so that the hybridization fertilization rate of the fugu obscurus female parent and the fugu rubripe male parent is improved, and the method has important reference significance for improving the hybridization fertilization rate of freshwater fishes and marine fishes.

Description

technical field [0001] The invention relates to the technical field of artificial insemination of fish, in particular to a method for improving the hybrid fertilization rate of puffer puffer obscurus females and puffer puffers obscura males. Background technique [0002] Puffer obscurus is a migratory fish in warm water and bottom rivers and seas, and it is only distributed in China and North Korea. China is produced in places such as the East China Sea, the Yellow Sea and the Bohai Sea. From March to June every year, they migrate upstream and lay eggs, fertilize and hatch in fresh water. [0003] The red fin puffer mainly inhabits the warm seawater environment at the bottom of the sea. It is mainly distributed in the Northwest Pacific Ocean, from the coasts of Japan, South Korea and Russia to the East China Sea. Taiwan of my country is distributed in the back, northeast and east waters. Spawns in the Haizhou Bay area from March to May every year, and reaches the peak prod...

Claims

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Application Information

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IPC IPC(8): A01K61/10
CPCA01K61/10Y02A40/81
Inventor 郭正龙钱晓明张巧云朱浩拥王耀辉王巧丽
Owner JIANGSU ZHONGYANG GRP
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