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Wheat-tritileymus 3D (3Ns) alien substitution line cultivation method and identification method

A technology of heterogenous line replacement and identification method, which is applied in the field of cultivation of new plant varieties, can solve the problems such as the lack of hybridization of durum wheat and edge wheat, and achieve the effect of effective and accurate identification.

Inactive Publication Date: 2014-04-02
NORTHWEST A & F UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Although the existing technology has made great technological progress in the breeding of new wheat varieties by crossing wheat, there is no report on the crossing of durum wheat and limpet wheat to obtain 3D (3Ns) substitution lines

Method used

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  • Wheat-tritileymus 3D (3Ns) alien substitution line cultivation method and identification method
  • Wheat-tritileymus 3D (3Ns) alien substitution line cultivation method and identification method
  • Wheat-tritileymus 3D (3Ns) alien substitution line cultivation method and identification method

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Experimental program
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Effect test

Embodiment 1

[0033] Embodiment 1: the cultivation of wheat-Bum wheat 3D (3Ns) different substitution lines

[0034] Cytochromosomal analysis in F1, F2, F3 and F4 generations using durum wheat (2n=4x=28, AABB) cultivar D4286 crossed with octoploid Triticum aureus M842-16 (2n=8x=56, AABBDDNsNs) Microscopic examination and genomic in situ hybridization (GISH) identification, screen for individual plants with a chromosome number of 2n=42 and containing the Ns genome chromosomes of Limegrain, and bag and self-bred the selected individual plants. In the F5 generation, bred A wheat-Bumley 3D (3Ns) heterosubstitution line containing two 3Ns chromosomes was named 10DM57.

Embodiment 2

[0035] Embodiment 2: the identification of the different substitution line of the wheat-Bum wheat 3D (3Ns) of embodiment 1

[0036] Cytogenetic analysis: D4286×M842-16 F5 progeny of wheat 3D (3Ns) heterosubstitution line 10DM50, germinated in the seed room, and when the root length reached 1-2cm, cut the root tip and put it in ice water at 0-4°C Pretreatment for 24 hours, fixed with Carnot’s fixative, enzymatic hydrolysis with 1% cellulase + 1% pectinase, stained with acetic acid carmine and pressed into slices; observation of pollen mother cells: young ears of the right age were taken from the field, fixed with Carnot’s fixative, and acetic acid carmine Stained sheet, observed under Olympus BX60 microscope; using durum wheat variety D4286 (AABB, 2n=28) to cross with M842-16 (AABBDDNsNs, 2n=56), the progeny were bagged and selfed for 4 generations, and in the F5 offspring, through the root Microscopic examination of somatic cell chromosomes of apical cells found a plant 10DM50...

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Abstract

The invention discloses a wheat-tritileymus 3D (3Ns) alien substitution line cultivation method, comprising the following steps: (1) hybridizing tetraploid durum wheat and octaploid tritileymus, authenticating every generation, and screening single plants with chromosome number (2n=42) and tritileymus Ns genome chromosomes; and (2) bagging the screened single plants, conducting selfing, and breeding a wheat-tritileymus alien substitution line with two tritileymus Ns genome chromosomes at the F5 generation. Furthermore, the invention also discloses an identification method for the quality of a wheat seed, and the identification method comprises a fluorescence in situ hybridization (FISH) method and an EST-STS (Expressed Sequence Tag-Sequence Tagged Sites) tag identification method. A wheat-tritileymus 3D (3Ns) alien substitution line cultured by using the method disclosed by the invention is remarkably improved on the aspects of agronomic traits such as plant height, ear length, tiller number and the like.

Description

technical field [0001] The invention relates to a method for cultivating new plant varieties, belonging to the fields of plant breeding and biological genetics. Background technique [0002] Wheat distant hybridization is one of the important ways and means to use exogenous genetic material to enrich the genetic basis of wheat and artificially create new species. Lime wheat (Leymus mollis (Trin.) Hara), also known as soft Lymus, belongs to Poaceae, Barley subfamily, Lymus is a wild weed, with drought resistance, cold resistance, salt resistance, barren resistance, resistance to a variety of fungi, Bacterial diseases, strong stalks, large spikes and multiple flowers are excellent germplasm resources for wheat crop variety improvement. The genome of Limegrass is NsNsXmXm, and the Ns genome comes from Psathyrostachys huashanica Keng, while the source of the Xm genome has not yet been determined. [0003] In the 1960s, Tsitsin et al. obtained hybrids of tetraploid and hexaploi...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H1/04C12Q1/68
Inventor 赵继新庞玉辉陈新宏武军程雪妮杨群慧
Owner NORTHWEST A & F UNIV
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