Method for making hyacinth root tip chromosome slice

A technology of chromosome preparation and narcissus, applied in the biological field, can solve the problems of complex ploidy of narcissus, and achieve the effects of simple and convenient operation, high accuracy and easy counting

Inactive Publication Date: 2011-09-14
SHENYANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the ploidy of narcissus is complex, with chromosome bases ranging from 7, 10, and 11. In addition to diploids, there are also triploids and tetraploids.

Method used

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  • Method for making hyacinth root tip chromosome slice
  • Method for making hyacinth root tip chromosome slice

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Successfully observed and counted the number of chromosomes of the daffodil 'Fortissimo'.

[0020] The specific implementation method is as follows:

[0021] A. Material collection: 1-1.5 cm root tip of vigorously growing potted daffodil 'Fortissimo' (Fortissimo) was taken at 9-10 am.

[0022] B. Low-temperature pretreatment: clean the removed root tips, put them into a colchicine solution with a concentration of 1 g / L, and pretreat them under refrigeration at 0-5°C for 4 hours.

[0023] C. Fixation: Rinse the pretreated new roots with distilled water for 3 times, blot dry, and fix with Carnot's fixative for 20 hours. Carnot's fixative is absolute ethanol: glacial acetic acid = 3:1.

[0024] D. Dissociation: Rinse the fixed new root with distilled water 3 times, blot dry, dissociate in 1mol / L hydrochloric acid 60 ℃ water bath for 10min, add 2-3 drops of 1mol / L sodium hydroxide (NaOH) Rinse 3 times with 500ml distilled water.

[0025] E. Staining: Place the dissociate...

Embodiment 2

[0029] Successfully observed and counted the number of chromosomes of the daffodil 'Arkle'.

[0030] The specific implementation method is the same as in Example 1. Microscopic examination results and photomicrographs see figure 2 .

[0031] The results of the above implementation and application on two representative daffodil varieties show that this method is an effective method for making daffodil chromosomes, and the method is simple and convenient to operate, the chromosomes are evenly dispersed and the background is light, and the number of chromosomes It is easy to count, and the number of chromosomes obtained by this method is highly accurate.

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Abstract

The invention provides a method for making a hyacinth root tip chromosome slice, which comprises the following steps: carrying out the low-temperature preprocessing by adopting a 1g / L colchicin solution; fixing by a Carnoy fixing solution; dissociating for 10 minutes by utilizing 1mol / L hydrochloric acid in a 60 DEG C water bath and rinsing by utilizing distilled water which is added with a small amount of NaOH; cutting the root tip into two parts along the longitudinal direction; dyeing for 10 minutes by utilizing a carbor fuchsin dyeing agent; rinsing by utilizing the distilled water after dyeing; making the slice by utilizing a tabletting method; and carrying out the microscopic examination and microphotograph under an Motic microscope. The method for making the slice provided by the invention has the advantages of simpleness and convenience in operation, uniform chromosome dispersion and shallow background; the number of chromosomes is easy to count; and the chromosome number accuracy which is obtained by adopting the slice making method provided by the invention is high.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for preparing chromosomes of daffodil root tips. Background technique [0002] Narcissus, also known as Dutch narcissus, is a collective name for new varieties of narcissus introduced from Europe to my country. It is a perennial herb of the Amaryllidaceae narcissus. There are about 40 original species in the world, most of which are native to the Mediterranean coast and central Europe. It has huge flowers, tall and straight flower stems, changeable coronas, and harmonious flower colors. It is a world-renowned bulbous flower and is widely cultivated and used all over the world. [0003] Chromosomes are the carriers of genes. The number, shape and structure of chromosomes determine the pairing behavior of chromosomes in cell meiosis; whether the pairing behavior is normal or not determines the fertility of plant microspores. Therefore, the structure and behavior ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28G01N1/30
Inventor 孙晓梅陆秀君杨宏光崔文山张丽杰孙琪佟潇
Owner SHENYANG AGRI UNIV
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