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Industrial production method of Cymbidium germchit based on sexual hybridization

A production method and a technology for Cymbidium cymbidium are applied in the field of industrial production of cymbidium cymbidium seedlings based on sexual hybridization, and can solve problems such as basic research to be carried out.

Inactive Publication Date: 2012-01-04
NANKAI UNIV
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  • Abstract
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Problems solved by technology

However, the hybrid breeding of Cymbidium in my country has just started, and a lot of basic research needs to be carried out.

Method used

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  • Industrial production method of Cymbidium germchit based on sexual hybridization
  • Industrial production method of Cymbidium germchit based on sexual hybridization
  • Industrial production method of Cymbidium germchit based on sexual hybridization

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Embodiment

[0029] A kind of industrial production method of Cymbidium cymbidium seedlings based on sexual hybridization provided by the present invention includes the steps: first, identify the chromosome number and ploidy of Cymbidium cymbidium parents; select hybrid combinations according to ploidy: carry out Sexual hybrid pollination of Cymbidium; post-pollination management of Cymbidium; asymbiotic germination and cultivation of Cymbidium seeds; induction and differentiation of protobulbs (PLBs) of Cymbidium seeds; seedling differentiation of Cymbidium ; Transplanting Cymbidium vase seedlings; Cultivation and management of transplanted Cymbidium seedlings.

[0030] The outstanding features and significant progress of the present invention can be embodied from the following examples, but the present invention will not be restricted in any way:

[0031] On January 28, 2003, a Japanese-made Cymbidium (variety number: MK1668⑥) was purchased from Beijing, numbered X IV. It was identified ...

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Abstract

The invention relates to an industrial production method of Cymbidium germchit based on sexual hybridization. The method provided by the invention comprises the following steps of: firstly identifying chromosome number and ploidy of Cymbidium parents; selecting pairing hybridized combinations according to the ploidy; carrying out a sexual hybridization pollination of the Cymbidium; managing the pollinated Cymbidium; carrying out a nonsymbiotic germination culture on Cymbidium seeds; inducing and differentiating protocorms (PLBs) of the Cymbidium seeds; differentiating bigger seedlings of the Cymbidium; transplanting the Cymbidium seedlings into bottles; and managing the cultivation of the Cymbidium transplanted seedlings. On the basis of indentifying the chromosomes of the Cymbidium parents, the method provided by the invention selectively pairs the hybridized combinations and carries out the sexual hybridization pollination, so that the aims of improving varieties and breeding new varieties are achieved; then a lot of Cymbidium germchit can be industrially produced through a tissue culture method.

Description

Technical field: [0001] The invention relates to the tissue culture propagation technology of precious flower seedlings, in particular to a sexual hybridization-based factory production method of Cymbidium grandiflora seedlings, which has important application value. Background technique: [0002] Cymbidium hybridmum (Cymbidium hybridmum) is the general term for hybrid varieties of the genus Cymbidium. It originated in the early 20th century and began to develop greatly in the 1940s and 1950s. By the Fifth World Orchid Congress in 1966, Cymbidium hybridmum The variety has won 40 medals in one fell swoop and entered the peak period of development. Because of its large flowers and rich colors, Cymbidium is deeply loved by Westerners, and it quickly developed in the West. By the 1970s, Japan, Singapore, South Korea and my country's Taiwan had also developed one after another. After the reform and opening up in mainland my country, Cymbidium has also been introduced, but most ...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01G31/00
Inventor 李秀兰陈力陈瑞阳
Owner NANKAI UNIV
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