Method for cultivating Lilium oriental hybrid embryos
A culturing method and hybrid embryo technology are applied in the field of cultivating hybrid embryos of Lily orientalis, which can solve problems such as hindering the development of lily breeding, and achieve the effects of shortening the breeding cycle and avoiding premature aging.
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Embodiment 1
[0021] Using the hybrid Oriental lily variety 'Sorbon' as the female parent and the Asian lily hybrid variety 'Reno' as the male parent, the capsules obtained 36 days after pollination were cut off and sterilized on the ultra-clean workbench The treatment is to soak the capsules in 75% alcohol for 1-2 seconds, then hold them with tweezers and put them over the flame of an alcohol lamp to burn them to sterilize the surface. Then peel off one side of the sterilized capsule in the petri dish, peel off the small embryo directly, and inoculate it into a medium composed of 1 / 2MS+BA 0.1mg / l+NAA 0.1mg / l+sucrose 50000mg / l, The inoculation ratio was 1:20g / ml, and then placed in a culture room at 23°C for 15 days to successfully germinate. After 3 months of continuous cultivation, a complete plant was cultivated, and a new lily variety 'Fangxin' was cultivated. The hybrid survival rate reached 91% using the above culture method.
[0022] Basic traits of 'Fangxin': yellow flowers, no spo...
Embodiment 2
[0024] Using the Oriental lily hybrid 'expression' as the female parent and the Asiatic lily hybrid 'Wolmi' as the male parent, the capsules obtained 40 days after pollination were cut off and sterilized on the ultra-clean workbench The treatment is to soak the capsules in 75% alcohol for 1-2 seconds, then hold them with tweezers and put them over the flame of an alcohol lamp to burn them to sterilize the surface. Then peel off one side of the sterilized capsule in the petri dish, peel off the small embryo directly, and inoculate it into a culture medium with a composition of 1 / 2MS+BA 0.2mg / l+NAA 0.05mg / l+sucrose 50000mg / l , the inoculation ratio was 1:30g / ml, and then placed in a culture room with a temperature of 27°C for 15 days to successfully germinate. After continuing to cultivate for 4 months, a complete plant was cultivated, and a new lily variety 'Dantong' was cultivated. The hybrid survival rate reached 94% using the above culture method.
[0025] ‘Dantong’ basic t...
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