Method for cultivating Lilium oriental hybrid embryos

A culturing method and hybrid embryo technology are applied in the field of cultivating hybrid embryos of Lily orientalis, which can solve problems such as hindering the development of lily breeding, and achieve the effects of shortening the breeding cycle and avoiding premature aging.

Inactive Publication Date: 2012-07-25
赵兴华
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  • Summary
  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

However, the cut flower varieties sold in the market are all imported varieties from abroad, and we do not have our own commercial varieties, so choosing the imported fine varieties of oriental lily for hybridization is an effective way for variety i

Method used

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Experimental program
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Effect test

Embodiment 1

[0021] Using the hybrid Oriental lily variety 'Sorbon' as the female parent and the Asian lily hybrid variety 'Reno' as the male parent, the capsules obtained 36 days after pollination were cut off and sterilized on the ultra-clean workbench The treatment is to soak the capsules in 75% alcohol for 1-2 seconds, then hold them with tweezers and put them over the flame of an alcohol lamp to burn them to sterilize the surface. Then peel off one side of the sterilized capsule in the petri dish, peel off the small embryo directly, and inoculate it into a medium composed of 1 / 2MS+BA 0.1mg / l+NAA 0.1mg / l+sucrose 50000mg / l, The inoculation ratio was 1:20g / ml, and then placed in a culture room at 23°C for 15 days to successfully germinate. After 3 months of continuous cultivation, a complete plant was cultivated, and a new lily variety 'Fangxin' was cultivated. The hybrid survival rate reached 91% using the above culture method.

[0022] Basic traits of 'Fangxin': yellow flowers, no spo...

Embodiment 2

[0024] Using the Oriental lily hybrid 'expression' as the female parent and the Asiatic lily hybrid 'Wolmi' as the male parent, the capsules obtained 40 days after pollination were cut off and sterilized on the ultra-clean workbench The treatment is to soak the capsules in 75% alcohol for 1-2 seconds, then hold them with tweezers and put them over the flame of an alcohol lamp to burn them to sterilize the surface. Then peel off one side of the sterilized capsule in the petri dish, peel off the small embryo directly, and inoculate it into a culture medium with a composition of 1 / 2MS+BA 0.2mg / l+NAA 0.05mg / l+sucrose 50000mg / l , the inoculation ratio was 1:30g / ml, and then placed in a culture room with a temperature of 27°C for 15 days to successfully germinate. After continuing to cultivate for 4 months, a complete plant was cultivated, and a new lily variety 'Dantong' was cultivated. The hybrid survival rate reached 94% using the above culture method.

[0025] ‘Dantong’ basic t...

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Abstract

The invention relates to a method for cultivating Lilium oriental hybrid embryos, which is effective in breeding effect and short in breeding period. The method includes: pollinating a Lilium oriental hybrid line; sectioning and peeling small embryos, ovules or ovaries; and performing isolated culture. By cross pollination of the ovaries and young embryos and isolated culture, the embryos can be taken out for culturing before abortion to avoid premature senility of distant hybrid embryos, and accordingly Lilium post-fertilization barrier can be overcome effectively, a great amount of distant hybrid embryos are allowed to continue growing into normal seeds, a breeding procedure is drawn close as soon as possible, and the breeding cycle is shortened. In addition, a great amount of distant hybrids are obtained, Lilium interspecific distant hybridization is achieved, and a batch of novel germplasm is created. The embryo rescue technology and the technology of within-bottle Lilium bulbing are combined, so that culture cycle of Lilium hybrids is shortened effectively, and hybrid survival rate is increased.

Description

Technical field: [0001] The invention relates to a method for cultivating hybrid embryos of oriental lilies. Background technique: [0002] Lily is an important ornamental plant with high economic value. Among them, Musk Lily (Longiflorum), Asiatic Lily (Asiatic), Oriental Lily (Oriental) and Trumpet Lily (Trumpet) are the four most commercially important lily hybrid lines, each with unique and excellent traits. Oriental lily has large flowers, bright colors and strong fragrance, and has strong resistance to gray mold (Botrytis). It is currently the most popular cut lily strain in China. However, the cut flower varieties sold in the market are all imported varieties from abroad, and we do not have our own commercial varieties. Therefore, it is an effective way to carry out variety improvement and rapid propagation of fine lily varieties by selecting good varieties of oriental lily that have been introduced for hybridization. Synthesizing the excellent traits of different h...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01H1/02
Inventor 吴海红赵兴华苏胜举杨佳明屈连伟印东生潘百涛于淼裴新辉周腰华李丹邢桂梅梅国宏
Owner 赵兴华
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