Method for preparing high-erucic acid brassica napus by utilizing artificially synthesized brassica napus

A Brassica napus, artificial synthesis technology, applied in the directions of botanical equipment and methods, applications, plant gene improvement, etc., can solve the problems of large breeding selection group, small variation range, large workload, etc., and achieves small breeding selection group, The effect of large variation and low workload

Inactive Publication Date: 2017-05-31
SOUTHWEST UNIV
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Problems solved by technology

[0004] The purpose of the present invention is to provide a breeding method created by high erucic acid Brassica napus materials, aiming to solve the problem that current high erucic acid materials are mainly obtained through conventional breeding methods by excavating existing Brassica napus germplasm resources. The method requires a large breeding selection group, a large workload, a small variation range, and troublesome operations

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  • Method for preparing high-erucic acid brassica napus by utilizing artificially synthesized brassica napus

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Embodiment 1

[0024] The method for preparing high erucic acid Brassica napus by artificially synthesizing Brassica napus provided in the embodiment of the present invention is to artificially create high-erucic rape (10D130) and high-quality, low-yield, high-yielding Brassica napus (Zhongshuang No. 11) Acid material; Specifically comprise the following steps:

[0025] 1. In the spring of 2010, 10D130 (artificially synthesized Brassica napus with an erucic acid content of more than 45% and an oil content of more than 40%) was used at the Xiema Experimental Base of Chongqing Rapeseed Engineering Center, Southwest University, Beibei District, Chongqing City (where all field experiments were conducted). ) and double-low quality Brassica napus variety Zhongshuang 11 (natural Brassica napus with erucic acid content less than 1% and oil content above 45%) were crossed to obtain the first-generation hybrid F 1 .

[0026] 2. Spring 2011, Hybrid F 1 F 2 A generation group with a total of 256 indi...

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Abstract

The invention discloses a method for preparing high-erucic acid brassica napus by utilizing artificially synthesized brassica napus. The method comprises the following steps: hybridizing the artificially synthesized brassica napus and natural brassica napus to expand the mutation range of compositions of fatty acid of rape seeds; obtaining a large amount of mutations to affect the metabolic pathway of the brassica napus by utilizing a large amount of exchange and recombination among chromosomes of a hybrid progeny of the artificially synthesized brassica napus and the natural brassica napus, and the missing, inserting, transposition and inactivation of a gene sequence, and obtaining a high-erucic acid material by utilizing mutation accumulation. According to the method, the breeding period is shortened through a generation-adding technology; only the materials with the erucic acid content of greater than 45 percent are selected, so that the workload is small; through directional selecting, the selecting pressure is increased generation by generation, so that the frequency of obtaining different types of high-erucic acid materials is improved; the obtained materials contain the compositions of the artificially synthesized brassica napus, so that the germplasm resources are expanded, and strong hybrid vigour is beneficially achieved in later breeding.

Description

technical field [0001] The invention relates to the technical field of new material breeding of Brassica napus, in particular to a method for preparing high erucic acid Brassica napus by artificially synthesizing Brassica napus. Background technique [0002] Erucic acid is a 22-carbon long-chain unsaturated fatty acid. It has the characteristics of wide application, high added value, large market demand and renewable. It is playing an increasingly important role in many modern industrial fields and is known as It is an important fine chemical raw material in the 21st century. Currently, erucic acid is mainly extracted from high erucic acid rapeseed oil. my country is a major producer of rapeseed in the world, and also a major producer of erucic acid. The annual output of erucic acid accounts for about 1 / 3 of the world. The products not only meet domestic demand, but are also exported to Japan, Europe and the United States and other countries. However, in recent years, the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02
CPCA01H1/02
Inventor 崔翠周清元唐章林李加纳殷家明王瑞任义英
Owner SOUTHWEST UNIV
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