Double male parent mixed pollen pollination crossbreeding method of Momordica charantia L.

Abstract

The invention discloses a double male parent mixed pollen pollination crossbreeding method ofMomordica charantia L. The method comprises the following steps: (1) selecting a female parent A and male parents B and C; (2) performing bagged isolation on female flowers and male flowers, blooming the next day, of the female parents and the male parents, mixing pollen of the male flowers of the male parents B and C in equal mass at 8 a.m. the next day, and pollinating stigmas of the female flowers of the female parent A, thereby obtaining a crossing combination A*(B+C); (3) planting fruits of first filial generation of single Momordica charantia L. with seeds reserved, and then observing the characters of fruits of F1 generation, wherein all the seeds of each single Momordica charantia L. are planted in one area, and all single plants in each area are subjected to self pollination; and (4) seeking single plates with the characters of the fruits of F1 generation different from those of the female parent A*the male parent B and the female parent A*the male parent C in each crossing area, and performing selfing on the obtained single plants with seeds reserved, namely performing seed selection, thereby obtaining a new breeding material of Momordica charantia L. The method creates a new path for innovation of breeding materials of Momordica charantia L., and a range of variation of existing breeding materials is effectively broadened.

Description

technical field [0001] The invention belongs to the technical field of crop genetics and breeding, and in particular relates to a hybrid breeding method for bitter gourd double male parents mixed pollen pollination. Background technique [0002] Bitter melon (Momordicacharantia L.) is an annual climbing herb of the family Cucurbitaceae. It originated in the tropical regions of Southeast Asia and is widely distributed in tropical, subtropical and temperate regions. It has a long history of cultivation in China, India, Japan and Southeast Asia. It was introduced in the Southern Song Dynasty After hundreds of years of cultivation in our country, rich types and varieties have been formed. It is mainly distributed in Guangdong, Guangxi, Hainan, Fujian, Taiwan, Sichuan, Hunan and other southern provinces. Pharmacological and pharmacological studies on bitter gourd have shown that bitter gourd has obvious pharmacological effects such as lowering blood sugar, anti-tumor, and anti-o...

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Problems solved by technology

[0010] At present, there are few reports on the breeding of bitter