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Method for creating intergeneric hybridization of radish and turnip

A technology of hybrids and turnips, applied in the field of creating hybrids between radishes and turnips, can solve the problems of hybrid authenticity identification, etc., and achieve the effects of enriching breeding foundation, improving compatibility, and promoting development

Active Publication Date: 2016-10-12
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The patent authorization announcement number published by Mei Shiyong et al. is CN 102210259 B, and the title of the invention is: a method for obtaining isolated embryos by distant hybridization between radish and turnip. The method of in vitro embryo culture with internal seed embryos has obtained a certain seedling rate, but the authenticity of the hybrid has not been identified

Method used

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  • Method for creating intergeneric hybridization of radish and turnip
  • Method for creating intergeneric hybridization of radish and turnip
  • Method for creating intergeneric hybridization of radish and turnip

Examples

Experimental program
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Effect test

Embodiment 1

[0038] A method for creating a hybrid between radish and turnip, specifically comprising the steps of:

[0039] 1. Sowing of parental materials: In order to meet the flowering period, in plastic greenhouses, sow radish materials in October and sow turnip materials in November; the sowing method is hole sowing, the plant spacing is 30cm, and the size and row spacing is 60×80cm; each hole is sown 2 to 3 seeds, after emergence, wait for the plant to grow to 5-10cm, then thin the seedlings, and keep one plant in each hole; conventional field management.

[0040] 2. Hybrid pollination at the flowering stage: From March to April of the following year, use radish as the female parent and turnip as the male parent, artificially emasculate, and use chemical agents to smear the stigma for pollination and hybridization at the bud stage; specifically:

[0041] (1) First arrange the inflorescence of the female parent, remove the flowers that have opened and the particularly small flower bu...

Embodiment 2

[0067] A kind of method for creating hybrid between radish and turnip genus of embodiment 2, concrete steps are the same as the method for creating hybrid between radish and turnip genus of embodiment 1, difference is:

[0068] The sowing method of the parent material in the step 1 is hole sowing, 2 to 3 seeds are sown in each hole, after emergence, the plants are grown to a certain size, and the seedlings are thinned, and one plant is reserved in each hole; conventional field management.

[0069] The hybrid pollination at the flowering stage in the step 2 is specifically:

[0070] (1) At first the female parent inflorescence is sorted out, and buds of suitable size are peeled off and detasseled;

[0071] (2) using a certain mass concentration of Gly to drip dip the stigma of the female parent before pollination;

[0072] (3) Take fresh pollen newly opened on the male plant, smear the stigma, bag the pollination, and mark the hybrid combination and pollination time.

[0073]...

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Abstract

The invention relates to a method for creating intergeneric hybridization of radish and turnip and belongs to the technical field of biotechnology breeding. The method specifically includes: sowing parent materials; performing cross pollination during the flowering stage; checking seeds; performing tissue culture to obtain hybrid seedlings; domesticating aseptic seedlings; performing hybrid morphological identification; performing hybrid cytological identification. The method has the advantages that according to the features that the distant hybridization of the radish and Brassica is severe in pre-fertilization barrier and minor in barrier after pollination, bud pollination is used and glycine of certain concentration is smeared to female parent stigmas before pollination to improve the compatibility of the intergeneric hybridization, natural pod bearing is used after the pollination, seed sterile culture is performed by the aid of the tissue culture technology, and the morphological and cytological identification of the obtained hybrid seedlings shows that the hybrid seedlings are actual intergeneric triploid hybrids.

Description

technical field [0001] The invention relates to a method for creating a hybrid between radish and turnip, and belongs to the technical field of biotechnology breeding. Background technique [0002] Radish is a close genus of Brassica in the Brassicaceae. Since the 1920s, through artificial hybridization, tissue culture, and somatic cell fusion, two species of radish-kale and radish-cabbage have been successfully synthesized. A new amphidiploid species was named as a new species, and a series of additional lines. Due to common problems such as reproductive isolation of distant hybrids, incompatibility of interspecific hybrids, hybrid abortion and hybrid sterility, in order to obtain sexual hybrids, people have adopted a variety of techniques and methods, including grafting, mixed pollination , cutting stigmas, chemical drug treatment, etc., but these techniques and methods have little effect on the distant hybridization of Brassica and radish. When Fang Xiangyou studied the...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H4/00
CPCA01H1/02A01H4/008
Inventor 娄丽娜苏小俊娄群峰许园园
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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