Molecular marker for identifying interspecific hybrids of Chinese cabbage and brassica carinata and separation conditions of A10 and C09 chromosomes of progeny materials

A molecular marker and chromosome technology, applied in the field of genetics and breeding, can solve problems such as time-consuming and labor-intensive, and achieve the effect of simple, fast, cost-effective, simple operation, and rich vegetable types.

Active Publication Date: 2019-11-08
INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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  • Claims
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AI Technical Summary

Benefits of technology

Inventions have been developed that allow researchers to easily distinguish different species from each other by comparing them through DNA analysis or protein expression techniques. These advancements make it easier than previous means because they are faster and more efficient compared to existing approaches like XRFID tags. They may even help predict future generations based on known relationships within these organisms. Overall, there will benefit both scientists who study crops and ethopians alike.

Problems solved by technology

This patented describes different ways to study farmers' ability to crossbred seeds from wild ones without causing any harm during harvesting. One method involves identifying isolated seedlings that cannot produce another type if it does develop multiple forms called pseudohybris (Pseudo Hybride). Another approach uses DNA marker analysis instead of traditional testing procedures like microspore counting. These approaches aim to provide accurate identification of remote sources while avoiding complicated experiments and equipment costs associated therewith.

Method used

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  • Molecular marker for identifying interspecific hybrids of Chinese cabbage and brassica carinata and separation conditions of A10 and C09 chromosomes of progeny materials
  • Molecular marker for identifying interspecific hybrids of Chinese cabbage and brassica carinata and separation conditions of A10 and C09 chromosomes of progeny materials
  • Molecular marker for identifying interspecific hybrids of Chinese cabbage and brassica carinata and separation conditions of A10 and C09 chromosomes of progeny materials

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1 This example identifies the hybrid F between Chinese cabbage and Ethiopian mustard 1 plant

[0043] 1.1 Extract the F to be detected 1 Genomic DNA of the plant and its parents.

[0044] 1.2 Synthetic primers:

[0045] C09D-F: 5'-TGACAGCATATGAAGCCTGC-3' (SEQ ID No.1);

[0046] C09D-R: 5'-GATCCTGCCACAAGAATTTGA-3' (SEQ ID No. 2).

[0047] 1.3PCR amplification. To be detected F 1 Plants and their parental DNA are used as templates, and the above primers are used for PCR amplification reaction. The reaction system is 10 μL, including: 1×PCR Buffer (containing Mg + ), 0.5ng template DNA, 0.2mM dNTPs, 0.5μM primer C09D-F, 0.5μM primer C09D-R, 1U Taq enzyme. PCR reaction conditions: 94°C for 3min; 94°C for 30s, 56.1°C for 30S, 72°C for 30S, 35 cycles; 72°C for 5min.

[0048] 1.4 The above PCR products were detected by polyacrylamide gel electrophoresis. Configure 8% polypropylene gel, run electrophoresis at 180 volts for 1.5 hours, and end the electrophoresi...

Embodiment 2

[0055] Example 2 This example identifies the hybrid backcross progeny between Chinese cabbage and Ethiopian mustard (BC 2 )Material

[0056] 1.1 Extract the genomic DNA of the plants to be tested and their parents.

[0057] 1.2 Synthetic primers:

[0058] C09D-F: 5'-TGACAGCATATGAAGCCTGC-3' (SEQ ID No.1);

[0059] C09D-R: 5'-GATCCTGCCACAAGAATTTGA-3' (SEQ ID No. 2).

[0060] 1.3PCR amplification. Taking the plant to be detected and its parental DNA as a template, the PCR amplification reaction is carried out with the above primers. The reaction system is 20 μL, including: 1×PCR Buffer (containing Mg + ), 1ng template DNA, 0.2mM dNTPs, 0.5μM primer C09D-F, 0.5μM primer C09D-R, 1U Taq enzyme. PCR reaction conditions: 95°C for 3min; 95°C for 30s, 56.1°C for 30S, 72°C for 30S, 35 cycles; 72°C for 10min.

[0061] 1.4 The above PCR products were detected by polyacrylamide gel electrophoresis. Configure 8% polypropylene gel, electrophoresis at 180 volts for 2 hours, and end the...

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Abstract

The invention discloses a molecular marker and method for identifying interspecific hybrids of Chinese cabbage and brassica carinata and tracking separation conditions of A10 and C09 chromosomes of progeny materials, and belongs to the field of plant genetic breeding. The marker is a pair of codominant molecular markers which can be used for identifying the authenticity of the interspecific hybrids of the Chinese cabbage and the brassica carinata, and can also be used for identifying and tracking the separation conditions of self-crossed progeny of distant hybrids, backcross descendants, chromosome addition lines, recombination segregation population and the A10 and C09 chromosomes of plants.

Description

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Claims

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Application Information

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Owner INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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