3 results about "Chromosome segregation" patented technology

We provide a 3D artificial kinetic chorodor scaffold. [Solution] A composition is provided comprising a three-dimensional artificial kinetochore scaffold comprising a unit complex containing NDC80 protein and NUF2 protein. The composition may be biodegradable or proteinaceous, and the number of unit complexes contained in the three-dimensional artificial kinetochore scaffold may be 10,000 to 50,000 or 20,000 to 40,000. The three-dimensional artificial kinetochore scaffold of this disclosure has the ability to align at the spindle equator during meiosis of an oocyte, for example. The three-dimensional artificial kinetochore scaffold of this disclosure can preferably be used, for example, to suppress premature chromosome segregation during meiosis of an oocyte.

Provided herein are compositions and methods for the treatment of cancer by activating the spindle assembly checkpoint (SAC) in cells. In particular, dimerized Mps1 and Spc105 / KNL1 constructs are provided as tunable activators of SAC, allowing for control of chromosome segregation accuracy and prevention of aneuploidies that are common in cancer.

Provided are a fusion protein comprising a mutated kinetochore protein and dCas9. The fusion protein is used in conjunction with guide RNAs target the fusion protein to a location of kinetochore assembly on a centromere such that the fusion protein interferes with chromosome segregation. Use of the fusion protein and the guide RNAs in cells results the cells acquiring an aneuploidy karyotype. Expression vectors that encode the fusion proteins and / or the guide RNAs and their uses in the method of producing an aneuploidy karyotype are also provided.