Molecular markers for identification of a02 and c02 chromosome segregation in hybrids between Chinese cabbage and Ethiopian mustard and their progeny

A technology of molecular markers and chromosomes, applied in the field of genetic breeding, can solve time-consuming and labor-intensive problems, achieve simple and fast cost, expand genetic resources, and reduce material costs

Active Publication Date: 2022-05-31
INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Due to the reproductive isolation of species, distant hybridization often requires techniques such as artificial pollination and embryo rescue, which is time-consuming and laborious, and requires certain scientific training

Method used

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  • Molecular markers for identification of a02 and c02 chromosome segregation in hybrids between Chinese cabbage and Ethiopian mustard and their progeny
  • Molecular markers for identification of a02 and c02 chromosome segregation in hybrids between Chinese cabbage and Ethiopian mustard and their progeny
  • Molecular markers for identification of a02 and c02 chromosome segregation in hybrids between Chinese cabbage and Ethiopian mustard and their progeny

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] CO2B-R: 5'-GCCATTTTGAACCATGAACC-3'.

[0043] 1.3 PCR amplification. to be detected F

[0044] 1.4 The above PCR products were detected by polyacrylamide gel electrophoresis. Configure 8% polypropylene gel, 180 volt electrophoresis 1.5

[0045] 1.5 Take out the above-mentioned polyacrylamide gel, silver staining, see Figure 1.

[0048] The F

Embodiment 2

[0052] 1.1 Extract the genomic DNA of the plants to be tested and their parents.

[0054] 1.3 PCR amplification. Taking the plants to be detected and their parental DNAs as templates, PCR amplification reactions were carried out with the above primers. opposite

[0055] 1.4 The above PCR products were detected by polyacrylamide gel electrophoresis. Configure 8% polypropylene gel, 180 volt electrophoresis for 2 hours

[0056] 1.5 Take out the above-mentioned polyacrylamide gel, silver staining, see Figure 4.

[0059] Whole-genome illumina sequencing was carried out to the No. 3 individual plant that was identified by molecular markers, and the sequencing depth was 10 ×;

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Abstract

The invention discloses a molecular marker and a method for identifying hybrids between Chinese cabbage and Ethiopian mustard and tracking the chromosomal separation of A02 and C02 offspring materials, belonging to the field of plant genetics and breeding. This marker is a pair of co-dominant molecular markers, which can be used to identify the authenticity of hybrids between Chinese cabbage and Ethiopian mustard, and can also be used to identify and track self-crossed offspring, backcrossed offspring, chromosome addition lines, and recombination of distant hybrids Chromosomal segregation of A02 and C02 in segregating populations and plants.

Description

Identification of hybrids between Chinese cabbage and Ethiopian mustard and their progeny materials by A02 and C02 staining Molecular markers for body separation technical field [0001] The present invention relates to the field of genetics and breeding, in particular, to a method for identifying and breeding distant hybrid plants. Background technique [0002] Distant hybridization is an important means to create new plant germplasm and expand breeding resources. Due to reproductive isolation of species, distant Hybridization often requires techniques such as artificial pollination and embryo rescue, which are time-consuming and labor-intensive, and require certain scientific training. in the process of outbreeding , due to incomplete emasculation, the development of female gametes into plants, and other reasons may result in the production of pseudohybrid plants. thus, Plants obtained by distant hybridization need to be tested for true hybrids by molecular and cyto...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/156
Inventor 张晓辉李锡香宋江萍王海平
Owner INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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