Molecular markers for identifying interspecific hybrids of Chinese kale and red cabbage and tracking chromosome segregation of a08 and c08 in their progeny

A technology of molecular markers and chromosomes, applied in the field of genetic breeding, can solve time-consuming and labor-intensive problems, achieve simple and fast cost, expand genetic resources, and transfer new traits

Active Publication Date: 2022-05-31
INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the reproductive isolation of species, distant hybridization often requires techniques such as artificial pollination and embryo rescue, which is time-consuming and laborious, and requires certain scientific training

Method used

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  • Molecular markers for identifying interspecific hybrids of Chinese kale and red cabbage and tracking chromosome segregation of a08 and c08 in their progeny
  • Molecular markers for identifying interspecific hybrids of Chinese kale and red cabbage and tracking chromosome segregation of a08 and c08 in their progeny
  • Molecular markers for identifying interspecific hybrids of Chinese kale and red cabbage and tracking chromosome segregation of a08 and c08 in their progeny

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] 1.3 PCR amplification. to be detected F

[0048] 1.4 The above PCR products were detected by polyacrylamide gel electrophoresis. Configure 8% polypropylene gel, 180 volt electrophoresis for 2 hours

[0049] 1.5 Take out the above-mentioned polyacrylamide gel, silver staining, see Figure 1.

[0052] The F

[0054] The F

Embodiment 2

[0057] 1.1 Extract the genomic DNA of the plants to be tested and their parents.

[0061] 1.3 PCR amplification. Taking the plants to be detected and their parental DNAs as templates, PCR amplification reactions were carried out with the above primers. opposite

[0062] 1.4 The above PCR products were detected by polyacrylamide gel electrophoresis. Configure 8% polypropylene gel, 180 volt electrophoresis 1.5

[0063] 1.5 Take out the above-mentioned polyacrylamide gel, silver staining, see Figure 4.

[0070]

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Abstract

The invention provides a molecular marker for identifying the segregation of chromosomes A08 and C08 of interspecific hybrids of kale and red cabbage and their offspring materials, and belongs to the field of plant genetics and breeding. The present invention provides molecular markers for identifying interspecific hybrids of kale and red cabbage and their progeny materials A08 and C08 chromosome segregation. It can also be used to identify selfed offspring, backcrossed offspring, chromosome addition lines, and recombined segregation populations and plants of distant hybrids.

Description

Identification of interspecific hybrids between kale and beetroot and tracking of their progeny materials A08 and C08 staining Molecular markers for body separation technical field [0001] The present invention relates to the field of genetics and breeding, in particular, to a method for identifying and breeding distant hybrid plants. Background technique [0002] Distant hybridization is an important means to create new plant germplasm and expand breeding resources. Due to reproductive isolation of species, distant Hybridization often requires techniques such as artificial pollination and embryo rescue, which are time-consuming and labor-intensive, and require certain scientific training. in the process of outbreeding , due to incomplete emasculation, the development of female gametes into plants, and other reasons may result in the production of pseudohybrid plants. thus, Plants obtained by distant hybridization need to be tested for true hybrids by molecular and c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/156
Inventor 张晓辉李锡香宋江萍王海平
Owner INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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